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1 Online-Ressource (123 Seiten, 6457 KB)
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Content:
Cells are built from a variety of macromolecules and metabolites. Both, the proteome and the metabolome are highly dynamic and responsive to environmental cues and developmental processes. But it is not their bare numbers, but their interactions that enable life. The protein-protein (PPI) and protein-metabolite interactions (PMI) facilitate and regulate all aspects of cell biology, from metabolism to mitosis. Therefore, the study of PPIs and PMIs and their dynamics in a cell-wide context is of great scientific interest. In this dissertation, I aim to chart a map of the dynamic PPIs and PMIs across metabolic and cellular transitions. As a model system, I study the shift from the fermentative to the respiratory growth, known as the diauxic shift, in the budding yeast Saccharomyces cerevisiae. To do so, I am applying a co-fractionation mass spectrometry (CF-MS) based method, dubbed protein metabolite interactions using size separation (PROMIS). PROMIS, as well as comparable methods, will be discussed in detail in chapter 1. Since ...
Note:
Dissertation Universität Potsdam 2023
Additional Edition:
Erscheint auch als Druck-Ausgabe Schlossarek, Dennis Identification of dynamic protein-metabolite complexes in saccharomyces cerevisiae using co-fractionation mass spectrometry Potsdam, 2023
Language:
English
Keywords:
Hochschulschrift
DOI:
10.25932/publishup-58282
URN:
urn:nbn:de:kobv:517-opus4-582826
Author information:
Willmitzer, Lothar 1952-
Author information:
Fernie, Alisdair 1974-
Author information:
Walther, Dirk 1972-
Author information:
Skirycz, Aleksandra 1980-
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