Kooperativer Bibliotheksverbund

UID:

Format: 1 online resource (XIV, 404 p. 80 illus., 48 illus. in color.)

Edition: 1st ed. 2013.

ISBN: 1-62703-487-0

Series Statement: Methods in Molecular Biology, 1033

Content: Membrane proteins and membrane lipids form complex interactive systems that are highly dynamic and able to be studied only by combinations of different in vivo and in vitro techniques.  In Membrane Biogenesis: Methods and Protocols, experts in the field present a broad collection of methods to study the biogenesis and function of cellular membranes. Beginning with how membrane lipids or membrane proteins can be studied, this detailed volume continues with sections covering different procedures to investigate the interaction of membrane proteins among each other or with membrane lipids, methods to study the biogenesis of membrane proteins and the dynamics of organelles, as well as protocols for the analyses of the functions or complex organization of membrane proteins.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Extensive and easily applicable, Membrane Biogenesis: Methods and Protocols provides readers with a comprehensive but still concise collection including both basic protocols of rather general application and more specialized methods for specific and novel techniques.

Note: Bibliographic Level Mode of Issuance: Monograph , Quantitative Analysis of Cellular Lipids by Nano-Electrospray Ionization Mass Spectrometry -- Thin-Layer Chromatography of Phospholipids -- Analysis of Membrane Lipid Biogenesis Pathways Using Yeast Genetics -- Using 2D Crystals to Analyze the Structure of Membrane Proteins -- Crystallization of Membrane Proteins -- Molecular Dynamics Simulations of Membrane Proteins -- Site-Specific Fluorescent Probe Labeling of Mitochondrial Membrane Proteins -- Topology Determination of Untagged Membrane Proteins -- self–assembling GFP: A Versatile Tool for Plant (Membrane) Protein Analyses -- The Use of Cardiolipin-Containing Liposomes as a Model System to Study the Interaction Between Proteins and the Inner Mitochondrial Membrane -- Analysis of the Interaction Between Membrane Proteins and Soluble Binding Partners by Surface Plasmon Resonance -- Peptide Interaction with and Insertion into Membranes -- Scanning Fluorescence Correlation Spectroscopy in Model Membrane Systems -- Analyses of Protein-Protein Interactions by In Vivo Photocrosslinking in Budding Yeast -- Sedimentation Velocity Analytical Ultracentrifugation in Hydrogenated and Deuterated Solvents for the Characterization of Membrane Proteins -- Membrane Partitioning and Translocation Studied by Isothermal Titration Calorimetry -- Analyzing Membrane Dynamics with Live Cell Fluorescence Microscopy with a Focus on Yeast Mitochondria -- Analysis of Protein Translocation into the Endoplasmic Reticulum of Human Cells -- An Assay to Monitor the Membrane Integration of Single-Span Proteins -- Methods to Study the Biogenesis of Membrane Proteins in Yeast Mitochondria -- Reconstitution of Mitochondrial Presequence Translocase into Proteoliposomes -- Single Channel Analysis of Membrane Proteins in Artificial Bilayer Membranes -- Quantification of Protein Complexes by Blue Native Electrophoresis -- Optimizing E. coli-Based Membrane Protein Production Using Lemo21(DE3) and GFP-Fusions. , English

Additional Edition: ISBN 1-62703-486-2

Language: English

DOI: 10.1007/978-1-62703-487-6

UID:

Format: 47 S.

Language: German

UID:

Format: 47 Seiten

Edition: Fünfte Auflage

Note: In Fraktur.

Language: German

Author information: Luther, Martin

UID:

Format: 1 online resource (352 p.)

ISBN: 9783110252361 , 9783110238570

Content: This book presents a survey of recent developments in protein biochemistry. Top researchers in the field of protein biochemistry describe modern methods to address the challenges of protein purification by three-phase partitioning, and their folding and degradation by the functions of chaperones. The significance of peptide purity for fibril formation is addressed as well as the use of target oriented peptide arrays in palliative approaches in mucoviszidose. The design and application of protein epitope mimetics just as the structural resolving of the misfolding of various mutant proteins in serpinopathies enlarge our tools in resolving pathophysiological imbalances.

Note: Frontmatter -- , Preface -- , Editor. List of contributing authors -- , Contents -- , Abbreviations -- , Acknowledgements -- , 1 Three-phase partitioning -- , 2 Folding and degradation functions of molecular chaperones -- , 3 Membrane protein folding in detergents -- , 4 Glycoprotein-folding quality control in the endoplasmic reticulum -- , 5 Conformational dynamics in peptides and proteins studied by triplet-triplet energy transfer -- , 6 Protein import into the intermembrane space of mitochondria -- , 7 On-membrane identification of gel-resolved proteins by matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) -- , 8 Analysis of protein complexes using chemical cross-linking and mass spectrometry -- , 9 Single-crystal spectroscopy correlated with X-ray crystallography provides complementary perspectives on macromolecular function -- , 10 Wide-angle X-ray solution scattering (WAXS) -- , 11 Where purity matters: recombinant versus synthetic peptides in beta amyloid formation -- , 12 Chemical modifi cation of proteins in living cells -- , 13 Proteomics of human bronchoalveolar lavage fluid: discovery of biomarkers of chronic obstructive pulmonary disease (COPD) with difference gel electrophoresis (DIGE ) and mass spectrometry (MS) -- , 14 Proteomic analysis of Duchenne muscular dystrophy (DMD) -- , 15 Target-oriented peptide arrays in a palliative approach to cystic fibrosis (CF) -- , 16 Probing protein dynamics in vivo using backbone cyclization: bacterial acyl carrier protein as a case study -- , 17 The protein epitope mimetic approach to protein-protein interaction inhibitors -- , 18 The structural biology of α1-antitrypsin deficiency and the serpinopathies -- , Index , Mode of access: Internet via World Wide Web. , In English.

In: DGBA Backlist Complete English Language 2000-2014 PART1, De Gruyter, 9783110238570

In: DGBA Backlist Medicine and Life Sciences 2000-2014 (EN), De Gruyter, 9783110238495

In: DGBA Medicine and Life Sciences 2000 - 2014, De Gruyter, 9783110637915

In: E-BOOK GESAMTPAKET / COMPLETE PACKAGE 2011, De Gruyter, 9783110261189

In: E-BOOK PACKAGE ENGLISH LANGUAGES TITLES 2011, De Gruyter, 9783110261233

In: E-BOOK PAKET SCIENCE TECHNOLOGY AND MEDICINE 2011, De Gruyter, 9783110261202

Additional Edition: ISBN 9783110252330

Language: English

Subjects: Biology

DOI: 10.1515/9783110252361

URL: https://doi.org/10.1515/9783110252361

URL: https://www.degruyter.com/isbn/9783110252361

URL: Cover

UID:

Format: 1 online resource (610 p.)

Edition: 1st ed. 2007.

ISBN: 1-280-97198-3 , 9786610971985 , 1-59745-365-X

Series Statement: Methods in Molecular Biology, 372

Content: Mitochondria: Practical Protocols offers a broad collection of methods for studying the molecular biology, function, and features of mitochondria. In the past decade, mitochondrial research has elucidated the important influence of mitochondrial processes on integral cell processes such as apoptosis and cellular aging. This practical guide presents a wide spectrum of mitochondrial methods, each written by specialists with solid experience and intended for implementation by novice and expert researchers alike. Part I introduces major experimental model systems and discusses their specific advantages and limitations for functional analysis of mitochondria. The concise overview of general properties of mitochondrial systems is supplemented by detailed protocols for cultivation of model organisms. Parts II-VI comprise a robust collection of protocols for studying different molecular aspects of mitochondrial functions including: genetics and microbiology, biochemistry, physiology, dynamics and morphology, and functional genomics. Emphasis is placed on new and emerging topics in mitochondrial study, such as the examination of apoptotic effects, fusion and fission of mitochondria, and proteome and transcriptome analysis.

Note: Description based upon print version of record. , Model Systems to Study Mitochondrial Functions -- The Mitochondria of Cultured Mammalian Cells -- The Mitochondria of Cultured Mammalian Cells -- Drosophila melanogaster as a Model System to Study Mitochondrial Biology -- Isolation and Functional Analysis of Mitochondria From the Nematode Caenorhabditis elegans -- Isolation of Mitochondria From Procyclic Trypanosoma brucei -- Saccharomyces cerevisiae as a Model Organism to Study Mitochondrial Biology -- Studying Mitochondria in an Attractive Model: Schizosaccharomyces pombe -- Neurospora crassa as a Model Organism for Mitochondrial Biogenesis -- Isolation of Intact, Functional Mitochondria From the Model Plant Arabidopsis thaliana -- Chlamydomonas reinhardtii: The Model of Choice to Study Mitochondria From Unicellular Photosynthetic Organisms -- Genetics and Molecular Biology -- Directed Alteration of Saccharomyces cerevisiae Mitochondrial DNA by Biolistic Transformation and Homologous Recombination -- Screens for Mitochondrial Mutants in Yeast -- Transcript End Mapping and Analysis of RNA Editing in Plant Mitochondria -- In Vitro Analysis of the Yeast Mitochondrial RNA Polymerase -- Functional Analysis by Inducible RNA Interference in Drosophila melanogaster -- Analysis of Replicating Mitochondrial DNA by Two-Dimensional Agarose Gel Electrophoresis -- Biochemistry -- The Analysis of tRNA Import Into Mammalian Mitochondria -- Analysis of Mitochondrial Protein Synthesis in Yeast -- Large-Scale Isolation of Mitochondrial Ribosomes From Mammalian Tissues -- Protein Import Into Isolated Mitochondria -- Analyzing Import Intermediates of Mitochondrial Proteins by Blue Native Gel Electrophoresis -- In Vitro and In Vivo Protein Import Into Plant Mitochondria -- Two-Dimensional Blue Native/Blue Native Polyacrylamide Gel Electrophoresis for the Characterization of Mitochondrial Protein Complexes and Supercomplexes -- Investigation of Iron-Sulfur Protein Maturation in Eukaryotes -- Studying Proteolysis Within Mitochondria -- Physiology -- Methods to Determine the Status of Mitochondrial ATP Synthase Assembly -- ATP Production in Isolated Mitochondria of Procyclic Trypanosoma brucei -- Oxidative Stress and Plant Mitochondria -- Measuring Mitochondrial Shape Changes and Their Consequences on Mitochondrial Involvement During Apoptosis -- Assessing Mitochondrial Potential, Calcium, and Redox State in Isolated Mammalian Cells Using Confocal Microscopy -- Dynamics and Morphology -- Fluorescence Imaging of Mitochondria in Yeast -- Mitochondrial Fusion In Vitro -- Conventional and Immunoelectron Microscopy of Mitochondria -- Mitochondrial Division in Caenorhabditis elegans -- Functional Genomics -- Yeast Mitochondrial Transcriptomics -- Plant Mitochondrial Transcriptomics by Quantitative RT-PCR -- Proteomics of Yeast Mitochondria -- Arabidopsis Mitochondrial Proteomics -- MitoP2, an Integrated Database for Mitochondrial Proteins. , English

Additional Edition: ISBN 1-62703-833-7

Additional Edition: ISBN 1-58829-667-9

Language: English

DOI: 10.1007/978-1-59745-365-3

UID:

Format: graph. Darst.

ISSN: 1040-4651

In: The plant cell, Rockville, Md. : Soc., 1989, 25(2013), 7, Seite 2647-2660, 1040-4651

In: volume:25

In: year:2013

In: number:7

In: pages:2647-2660

Language: English