Virology, August, 2014, Vol.462-463, p.189(10)
To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.virol.2014.06.002 Byline: Katharina Hipp, Peter Rau, Benjamin Schafer, Bruno Gronenborn, Holger Jeske Abstract: Geminiviruses, single-stranded DNA plant viruses, encode a replication-initiator protein (Rep) that is indispensable for virus replication. A potential cyclin interaction motif (RXL) in the sequence of African cassava mosaic virus Rep may be an alternative link to cell cycle controls to the known interaction with plant homologs of retinoblastoma protein (pRBR). Mutation of this motif abrogated rereplication in fission yeast induced by expression of wildtype Rep suggesting that Rep interacts via its RXL motif with one or several yeast proteins. The RXL motif is essential for viral infection of Nicotiana benthamiana plants, since mutation of this motif in infectious clones prevented any symptomatic infection. The cell-cycle link (Clink) protein of a nanovirus (faba bean necrotic yellows virus) was investigated that activates the cell cycle by binding via its LXCXE motif to pRBR. Expression of wildtype Clink and a Clink mutant deficient in pRBR-binding did not trigger rereplication in fission yeast. Author Affiliation: (a) Institut fur Biomaterialien und biomolekulare Systeme, Abteilung fur Molekularbiologie und Virologie der Pflanzen, Universitat Stuttgart, Pfaffenwaldring 57, D-70550 Stuttgart, Germany (b) Institut des Sciences du Vegetal, CNRS, 91198 Gif-sur-Yvette, France
Virus Diseases -- Health Aspects ; Plants (Organisms) -- Health Aspects ; Proteins -- Health Aspects ; Dna -- Health Aspects
Cengage Learning, Inc.