Format:
Online-Ressource (XXXIV, 284 S.)
Edition:
Online-Ausg. 2009 Electronic reproduction; Available via World Wide Web
ISBN:
0123737494
,
9780123737496
Series Statement:
Methods in enzymology 421
Content:
The critically acclaimed laboratory standard for more than fifty years, Methods in Enzymologyis one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 400 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences. This new volume presents methods related to the use of bacterial genetics for genomic engineering. The book includes sections on strain collections and genetic nomenclature, transposons, and phage.
Note:
Literaturangaben
,
Front Cover; Advanced Bacterial Genetics: Use of Transposons and Phage for Genomic Engineering; Copyright Page; Table of Contents; Contributors to Volume 421; Preface; Volumes in Series; Section I: Strain Collections and Genetic Nomenclature; Chapter 1: Strain Collections and Genetic Nomenclature; Introduction; Genotype; Phenotype; References; Section II: Transposons; Chapter 2: Use of Antibiotic-Resistant Transposons for Mutagenesis; Introduction; Transposase; Delivery of Transposons; Transposon Pools; References; Chapter 3: In Vivo Mutagenesis Using EZ-Tn5TM; Introduction; Protocol
,
ReferencesChapter 4: Identification of Essential Genes in Bacteria; Introduction; General Considerations; Plan of the Experiment; Procedures; References; Chapter 5: Isolation of Transposon Insertions; Introduction; References; Chapter 6: Localized Mutagenesis; Introduction; Hydroxylamine Mutagenesis; Mutagenesis with Diethylsulfate; Hydroxylamine Mutagenesis In Vitro; Reagents; References; Chapter 7: Generation of Deletions and Duplications Using Transposons as Portable Regions of Homology with Emphasis on Mud and Tn10 Transposons; Introduction; General Considerations; References
,
Chapter 8: Target-Directed Proteolysis In VivoIntroduction; TEV Protease; TEV Protease Expression Vectors; Applications of TDP; Additional Practical Considerations; Additional Potential Applications; Acknowledgments; References; Chapter 9: Sets of Transposon-Generated Sequence-Tagged Mutants for Structure-Function Analysis and Engineering; Introduction; Permissive Sites; Beyond Permissive Sites; Flexibility of Insertion Size and Sequence; Engineering Permissive Sites with Other Functional Motifs; Creation of Derivatives from Permissive i31 Mutants; Acknowledgments; References
,
Chapter 10: Using Genomic Microarrays to Study Insertional/Transposon Mutant LibrariesIntroduction; Step 1. Generating Insertional Mutant Libraries; Step 2. Screening Mutant Libraries: A Primer; Step 3. Microarray Analysis of Mutant Pools and Mapping Transposon Insertions; Discussion and Conclusions; Acknowledgement; References; Chapter 11: Screening Transposon Mutant Libraries Using Full-Genome Oligonucleotide Microarrays; Introduction; Principle; Transposon Mutagenesis Protocol; Library Construction Protocol; Competitive Outgrowth of Mutant Libraries Protocol; Mutant Labeling Protocol
,
Microarray Design and Hybridization ProtocolData Analysis; Conclusion; Acknowledgment; References; Chapter 12: Creating Recombination-Activated Genes and Sequence-Defined Mutant Libraries Using Transposons; Introduction; Transposon Tn5 Derivatives; Recombination-Activated Alleles of Reporter Genes; Large-Scale Transposon Mutant Library Construction; High-Throughput Mapping of Transposon Insertions; Acknowledgments; References; Chapter 13: Use of Operon and Gene Fusions to Study Gene Regulation in Salmonella; Introduction; Mud Fusions; The Plan of the Experiment; Procedures; References
,
Chapter 14: Genomic Screening for Regulatory Genes Using the T-POP Transposon
,
Electronic reproduction; Available via World Wide Web
Additional Edition:
Druckausg. Advanced bacterial genetics Amsterdam : Elsevier, Academic Press, 2007 ISBN 0123737494
Additional Edition:
ISBN 9780123737496
Language:
English
Subjects:
Biology
Keywords:
Gentechnologie
;
Transposon
;
Gentechnologie
;
Bakteriophagen
;
Prokaryoten
;
Eukaryoten
;
Transposon
;
Bakterien
;
Transposon
;
Aufsatzsammlung
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