Format:
Online-Ressource (206 p)
ISBN:
9780128021163
Series Statement:
Advances in Planar Lipid Bilayers and Liposomes v.Volume 21
Content:
The Elsevier book-series 'Advances in Planar Lipid Bilayers and Liposomes' (APLBL) provides a global platform for a broad community of experimental and theoretical researchers studying cell membranes, lipid model membranes and lipid self-assemblies from the micro- to the nanoscale. Planar lipid bilayers are widely studied due to their ubiquity in nature and find their application in the formulation of biomimetic model membranes and in the design of artificial dispersion of liposomes. Moreover, lipids self-assemble into a wide range of other structures including micelles and the liquid crystalline hexagonal and cubic phases. Consensus has been reached that curved membrane phases do play an important role in nature as well, especially in dynamic processes such as vesicles fusion and cell communication. Self-assembled lipid structures have enormous potential as dynamic materials ranging from artificial lipid membranes to cell membranes, from biosensing to controlled drug delivery, from pharmaceutical formulations to novel food products to mention a few. An assortment of chapters in APLBL represents both an original research as well as comprehensives reviews written by world leading experts and young researchers.The APLBL book series gives a survey on recent theoretical as well as experimental results on lipid micro and nanostructures.In addition, the potential use of the basic knowledge in applications like clinically relevant diagnostic and therapeutic procedures, biotechnology, pharmaceutical engineering and food products is presented.An assortment of chapters in APLBL represents both an original research as well as comprehensives reviews written by world leading experts and young researchers.
Note:
Description based upon print version of record
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Front Cover; Advances in Planar Lipid Bilayers and Liposomes; Copyright; Contents; Contributors; Preface; Chapter One: Development of Polymer/Nanodiamond Composite Coatings to Control Cell Adhesion, Growth, and Functions; 1. Introduction; 2. Materials and Methods; 2.1. Synthesis of polymer/nanodiamond composite layers; 2.2. Surface characterization of the composite layers; 2.3. Cell culture experiments; 2.3.1. Cell cultures; 2.3.2. Cell adhesion assay; 2.3.3. Scanning electron microscopy; 2.3.4. Immunofluorescent labeling of the actin cytoskeleton; 2.3.5. Cell proliferation assay
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2.3.6. ALP activity assay2.4. Statistical analysis; 3. Results; 3.1. Characterization of the composite layers; 3.2. Cell adhesion; 3.3. Organization of the actin cytoskeleton; 3.4. Cell proliferation; 3.5. ALP activity; 4. Discussion; 5. Conclusions; Acknowledgments; References; Chapter Two: Tethered Phospholipid Bilayer Membranes: An Interpretation of the Electrochemical Impedance Response; 1. Introduction; 2. Electrochemical Impedance Spectroscopy; 2.1. Definition; 2.2. Equivalent circuit models of impedance; 2.3. Electrochemical impedance of defect-free tBLMs
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3. Electrochemical Impedance Response of Defects3.1. Phenomenological background for EI model; 3.2. Impedance of submembrane reservoir; 3.3. Total impedance of tBLMs containing defects; 4. tBLM Parameters That Determine EI Response; 4.1. Density of defects; 4.2. Specific resistance of the submembrane reservoir; 4.3. Helmholtz capacitance; 4.4. Phospholipid bilayer capacitance; 4.5. Clustering of defects; 5. Conclusions; Acknowledgments; References; Chapter Three: Microscopy of Model Membranes: Understanding How Bcl-2 Proteins Mediate Apoptosis; 1. Introduction
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2. Model Membranes and Techniques in Studying Bcl-2 Proteins2.1. Lipids of the mitochondria; 2.2. Supported lipid bilayers; 2.3. Liposomes; 2.3.1. Large unilamellar vesicles; 2.3.2. Giant unilamellar vesicles; 2.4. Techniques to study model membranes and membrane proteins; 2.4.1. Atomic force microscopy; 2.4.2. Fluorescence-based assays and fluorescence microscopy; 2.4.3. Fluorescence correlation spectroscopy; 2.4.3.1. Scanning fluorescence correlation spectroscopy; 2.4.3.2. Two-color fluorescence cross-correlation spectroscopy; 3. Uncovering the Mechanisms of the Bcl-2 Family
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3.1. Mechanistic differences in membrane permeabilization of Bcl-2 family members: Bax versus Bcl-xL3.2. Bax and Bak form pores of tunable size; 3.3. Peptides of Bax mimic its pore-forming activity by decreasing line tension in membranes; 3.4. Role of membranes in promoting interaction between Bcl-2 family members; 4. Summary and Future Directions; References; Chapter Four: Optical Microscopy of Giant Vesicles as a Tool to Reveal the Mechanism of Action of Antimicrobial Peptides and...; 1. Introduction; 2. Experimental Approaches Based on Optical Microscopy of GUVs
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2.1. Visualizing the peptide-induced leakage
Additional Edition:
9780128022016
Additional Edition:
9780128021163
Additional Edition:
Erscheint auch als Druck-Ausgabe Advances in Planar Lipid Bilayers and Liposomes
Language:
English
URL:
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