Pharmaceutical Research, 2010, Vol.27(12), pp.2614-2623
Byline: Jesper Astergaard (1), Emil Meng-Lund (1), Susan Weng Larsen (1), Claus Larsen (1), Karsten Petersson (2), James Lenke (3), Henrik Jensen (1) Keywords: diffusion; in vitro release; transdermal delivery; UV imaging Abstract: Purpose This study was conducted to characterize UV imaging as a platform for performing in vitro release studies using Nicorette(r) nicotine patches as a model drug delivery system. Methods The rate of nicotine release from 2 mm diameter patch samples (Nicorette(r)) into 0.067 M phosphate buffer, pH 7.40, was studied by UV imaging (Actipix SDI300 dissolution imaging system) at 254 nm. The release rates were compared to those obtained using the paddle-over-disk method. Results Calibration curves were successfully established which allowed temporally and spatially resolved quantification of nicotine. Release profiles obtained from UV imaging were in qualitative agreement with results from the paddle-over-disk release method. Conclusion Visualization as well as quantification of nicotine concentration gradients was achieved by UV imaging in real time. UV imaging has the potential to become an important technology platform for conducting in vitro drug release studies. Author Affiliation: (1) Department of Pharmaceutics and Analytical Chemistry Faculty of Pharmaceutical Sciences, University of Copenhagen, Universitetsparken 2, 2100, Copenhagen, Denmark (2) New Products, LEO Pharma A/S, Ballerup, Denmark (3) Paraytec Ltd., York, UK Article History: Registration Date: 19/08/2010 Received Date: 21/06/2010 Accepted Date: 19/08/2010 Online Date: 02/09/2010 Article note: Electronic Supplementary Material The online version of this article (doi: 10.1007/s11095-010-0257-9) contains supplementary material, which is available to authorized users.
diffusion ; release ; transdermal delivery ; UV imaging
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