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  • 1
    Language: German
    In: Sozial Extra, 2014, Vol.38(5), pp.28-33
    Description: Offene Jugendarbeit ist in Nürnberg ein zentrales Handlungsfeld der Kinder- und Jugendhilfe und wird quantitativ und qualitativ weiterentwickelt. Politische Bildung ist darin konzeptionell verankert und orientiert sich an den Interessen und Alltagserfahrungen der jungen Menschen. Damit kann Offene Jugendarbeit zum Ausgangspunkt für politische Bildung und von Partizipation sozial benachteiligter Jugendlicher werden.
    Keywords: Social Sciences ; Social Sciences, General ; Education, General ; Social Work ; Offene Jugendarbeit ; Kommunale Jugendarbeit ; Nürnberg ; Nationalsozialismus ; Partizipation ; Social Welfare & Social Work;
    ISSN: 0931-279X
    E-ISSN: 1863-8953
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  • 2
    In: PLoS ONE, 2014, Vol.9(12)
    Description: In the future, periods of strongly increased temperature in concert with drought (heat waves) will have potentially detrimental effects on trees and forests in Central Europe. Norway spruce might be at risk in the future climate of Central Europe. However, Douglas-fir is often discussed as an alternative for the drought and heat sensitive Norway spruce, because some provenances are considered to be well adapted to drier and warmer conditions. In this study, we identified the physiological and growth responses of seedlings from two different Douglas-fir provenances to increased temperature and atmospheric drought during a period of 92 days. We analysed (i) plant biomass, (ii) carbon stable isotope composition as an indicator for time integrated intrinsic water use efficiency, (iii) apparent respiratory carbon isotope fractionation as well as (iv) the profile of polar low molecular metabolites. Plant biomass was only slightly affected by increased temperatures and atmospheric drought but the more negative apparent respiratory fractionation indicated a temperature-dependent decrease in the commitment of substrate to the tricarboxylic acid cycle. The metabolite profile revealed that the simulated heat wave induced a switch in stress protecting compounds from proline to polyols. We conclude that metabolic acclimation successfully contributes to maintain functioning and physiological activity in seedlings of both Douglas-fir provenances under conditions that are expected during heat waves (i.e. elevated temperatures and atmospheric drought). Douglas-fir might be a potentially important tree species for forestry in Central Europe under changing climatic conditions.
    Keywords: Research Article ; Biology And Life Sciences ; Ecology And Environmental Sciences
    E-ISSN: 1932-6203
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  • 3
    Language: English
    In: PLoS ONE, 2011, Vol.6(9), p.e25282
    Description: Crystallization conditions of an intact monoclonal IgG4 (immunoglobulin G, subclass 4) antibody were established in vapor diffusion mode by sparse matrix screening and subsequent optimization. The procedure was transferred to microbatch conditions and a phase diagram was built showing surprisingly low solubility of the antibody at equilibrium. With up-scaling to process scale in mind, purification efficiency of the crystallization step was investigated. Added model protein contaminants were excluded from the crystals to more than 95%. No measurable loss of Fc-binding activity was observed in the crystallized and redissolved antibody. Conditions could be adapted to crystallize the antibody directly from concentrated and diafiltrated cell culture supernatant, showing purification efficiency similar to that of Protein A chromatography. We conclude that crystallization has the potential to be included in downstream processing as a low-cost purification or formulation step.
    Keywords: Research Article ; Biology ; Physics ; Physics ; Biochemistry
    E-ISSN: 1932-6203
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  • 4
    Language: English
    In: Analytical and Bioanalytical Chemistry, 2015, Vol.407(13), pp.3555-3566
    Description: Modified nucleosides derived from the RNA metabolism constitute an important chemical class, which are discussed as potential biomarkers in the detection of mammalian breast cancer. Not only the variability of modifications, but also the complexity of biological matrices such as urinary samples poses challenges in the analysis of modified nucleosides. In the present work, a comprehensive two-dimensional liquid chromatography mass spectrometry (2D-LC-MS) approach for the analysis of modified nucleosides in biological samples was established. For prepurification of urinary samples and cell culture supernatants, we performed a cis -diol specific affinity chromatography using boronate-derivatized polyacrylamide gel. In order to establish a 2D-LC method, we tested numerous column combinations and chromatographic conditions. In order to determine the target compounds, we coupled the 2D-LC setup to a triple quadrupole mass spectrometer performing full scans, neutral loss scans, and multiple reaction monitoring (MRM). The combination of a Zorbax Eclipse Plus C18 column with a Zorbax Bonus-RP column was found to deliver a high degree of orthogonality and adequate separation. By application of 2D-LC-MS approaches, we were able to detect 28 target compounds from RNA metabolism and crosslinked pathways in urinary samples and 26 target compounds in cell culture supernatants, respectively. This is the first demonstration of the applicability and benefit of 2D-LC-MS for the targeted metabolome analysis of modified nucleosides and compounds from crosslinked pathways in different biological matrices.
    Keywords: Modified nucleosides ; Metabolomics ; Two-dimensional liquid chromatography ; 2D-LC-MS ; Comprehensive 2D-LC ; Breast cancer
    ISSN: 1618-2642
    E-ISSN: 1618-2650
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  • 5
    Language: German
    In: unsere jugend, 2012, Vol.64(5), pp.221-225
    Description: Welche Rolle spiel Familienbildung in Großstadt-Jugendämtern? Der folgende Beitrag stellt Ergebnisse einer Umfrage zu dem Thema dar.
    Keywords: Großstadt ; Familienbildung ; Jugendamt;
    ISSN: unsere jugend
    E-ISSN: 0342-5258
    Source: CrossRef
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  • 6
    Language: German
    In: unsere jugend, 2011, Vol.63(7+8), pp.296-305
    Description: Mit dem Generalthema "Chancen und Herausforderungen der Kinder- und Jugendarbeit" fand das Nürnberger Forum der Kinder- und Jugendarbeit im September 2010 nun zum 20. Mal statt. Die Veranstaltungsreihe besteht seit 1989 kontinuierlich, mit einer dreijährigen Unterbrechung, als überregional ausgerichtete dreitägige Fachtagung.
    Keywords: Gemeinde (Kommune) ; Jugendarbeit ; Tagungsbericht ; Theorie-Praxis-Beziehung;
    ISSN: unsere jugend
    E-ISSN: 0342-5258
    Source: CrossRef
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  • 7
    Language: English
    In: PLoS ONE, Dec 1, 2014, Vol.9(12)
    Description: In the future, periods of strongly increased temperature in concert with drought (heat waves) will have potentially detrimental effects on trees and forests in Central Europe. Norway spruce might be at risk in the future climate of Central Europe. However, Douglas-fir is often discussed as an alternative for the drought and heat sensitive Norway spruce, because some provenances are considered to be well adapted to drier and warmer conditions. In this study, we identified the physiological and growth responses of seedlings from two different Douglas-fir provenances to increased temperature and atmospheric drought during a period of 92 days. We analysed (i) plant biomass, (ii) carbon stable isotope composition as an indicator for time integrated intrinsic water use efficiency, (iii) apparent respiratory carbon isotope fractionation as well as (iv) the profile of polar low molecular metabolites. Plant biomass was only slightly affected by increased temperatures and atmospheric drought but the more negative apparent respiratory fractionation indicated a temperature-dependent decrease in the commitment of substrate to the tricarboxylic acid cycle. The metabolite profile revealed that the simulated heat wave induced a switch in stress protecting compounds from proline to polyols. We conclude that metabolic acclimation successfully contributes to maintain functioning and physiological activity in seedlings of both Douglas-fir provenances under conditions that are expected during heat waves (i.e. elevated temperatures and atmospheric drought). Douglas-fir might be a potentially important tree species for forestry in Central Europe under changing climatic conditions.
    Keywords: Polyols – Exhibitions
    ISSN: 1932-6203
    Source: Cengage Learning, Inc.
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  • 8
    Language: English
    In: Analytica Chimica Acta, 21 June 2016, Vol.925, pp.34-42
    Description: In recent years, knowledge about metabolite changes which are characteristic for the physiologic state of cancer cells has been acquired by liquid chromatography coupled to mass spectrometry. Distinct molecularly characterized breast cancer cell lines provide an unbiased and standardized tumor model reflecting the heterogeneity of the disease. Tandem mass spectrometry is a widely applied analytical platform and highly sensitive technique for analysis of complex biological samples. Endo- and exometabolite analysis of the breast cancer cell lines MDA-MB-231, -453 and BT-474 as well as the breast epithelial cell line MCF-10A has been performed using two different analytical platforms: UPLC-ESI-Q-TOF based on a scheduled precursor list has been applied for highlighting of significant differences between cell lines and HPLC-ESI-QqQ using multiple reaction monitoring has been utilized for a targeted approach focusing on RNA metabolism and interconnected pathways, respectively. Statistical analysis enabled a clear discrimination of the breast epithelial from the breast cancer cell lines. As an effect of oxidative stress, a decreased GSH/GSSG ratio has been detected in breast cancer cell lines. The triple negative breast cancer cell line MDA-MB-231 showed an elevation in nicotinamide, 1-ribosyl-nicotinamide and NAD+ reflecting the increased energy demand in triple negative breast cancer, which has a more aggressive clinical course than other forms of breast cancer. Obtained distinct metabolite pattern could be correlated with distinct molecular characteristics of breast cancer cells. Results and methodology of this preliminary study could be transferred to studies with breast cancer patients.
    Keywords: Metabolomics ; Breast Cancer ; Quadrupole Time-of-Flight ; Liquid Chromatography ; Mass Spectrometry ; Chemistry
    ISSN: 0003-2670
    E-ISSN: 1873-4324
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  • 9
    Language: English
    In: Analytical Biochemistry, 2005, Vol.339(2), pp.297-309
    Description: Many physiological processes show a high degree of stereoselectivity, including the metabolism of xenobiotics as catalyzed by cytochrome P450 enzymes. An analysis of these chiral discrimination effects in drug metabolism is essential for an in-depth understanding of metabolic pathways that differ between enantiomers of a given chiral drug or metabolite thereof. Achiral chromatographic separation and structural identification followed by chiral analysis of metabolites from blood specimens usually requires a time-consuming multistage analytical technique. In an effort to optimize such a complicated analytical scheme, a novel two-dimensional online achiral–chiral liquid chromatography–tandem mass spectrometry (LC/LC–MS/MS) coupling method was developed by using a peak parking technique in combination with a makeup flow system. Metabolites were separated in the first dimension using a C18 reversed-phase system. A makeup eluent of water/methanol (95/5) was split into the flow before storing the metabolites separately on chiral cartridges. Subsequently, the metabolite enantiomers were eluted backward onto the analytical chiral column and separated, and the ratio of enantiomers was determined. The method was successfully validated with respect to limit of detection, linearity, intra- and interday accuracy, and precision. In the course of a human volunteer study investigating the influence of CYP (cytochrome) 2C9 genetic polymorphism on phenprocoumon (PPC) metabolism, we used this new two-dimensional online analytical technique for the analysis of PPC metabolites in plasma. The enantiomeric forms of 4′-, 6-, and 7-hydroxy-PPC metabolites as well as two novel metabolites were identified, and the ratio of the enantiomers was calculated. We found that the enantiomeric ratio for the different metabolites in the plasma sample of each measured individual differs markedly from a nearly 100% chiral discrimination for the two new putative metabolites. This new analytical coupling method possesses general utility in the analysis of chiral discrimination effects, particularly as it relates to pharmacokinetics and dynamics, a scientific field that is rapidly becoming an area of concern and interest.
    Keywords: Phenprocoumon ; Metabolites ; Chiral Separation ; Achiral–Chiral LC/LC–MS/MS Coupling ; Chiral Discrimination in Drug Metabolism ; Chemistry ; Anatomy & Physiology
    ISSN: 0003-2697
    E-ISSN: 1096-0309
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  • 10
    In: Applied and Environmental Microbiology, 2010, Vol. 76(12), p.4008
    ISSN: 0099-2240
    ISSN: 00992240
    Source: American Society of Microbiology
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