Analytical and Bioanalytical Chemistry, July, 2011, Vol.400(9), p.2755(7)
Byline: Anne Marz (1), Bettina Monch (2), Petra Rosch (1), Michael Kiehntopf (2), Thomas Henkel (3), Jurgen Popp (1,3) Keywords: SERS; Microfluidic lab-on-a-chip; Enzyme activity; Thiopurine methyltransferase; 6-Mercaptopurine Abstract: In this contribution, the great potential of surface enhanced Raman spectroscopy (SERS) in a lab-on-a-chip (LOC) device for the detection of analyte molecules in a complex environment is demonstrated. Using LOC-SERS, the enzyme activity of thiopurine S-methyltransferase (TPMT) is analysed and identified in lysed red blood cells. The conversion of 6-mercaptopurine to 6-methylmercaptopurine catalysed by TPMT is observed as it gives evidence for the enzyme activity. Being able to determine the TPMT activity before starting a treatment using 6-mercaptopurine, an optimized dosage can be applied to each patient and serious toxicity appearing within thiopurine treatment will be prevented. Author Affiliation: (1) Institute of Physical Chemisty, Friedrich Schiller University, Helmholtzweg 4, 07743, Jena, Germany (2) Department of Clinical Chemistry and Laboratory Diagnostics, University Hospital Jena, Erlanger Allee 101, 07747, Jena, Germany (3) Institute of Photonic Technology (IPHT), Albert Einstein Strasse 9, 07745, Jena, Germany Article History: Registration Date: 15/02/2011 Received Date: 26/11/2010 Accepted Date: 14/02/2011 Online Date: 26/02/2011 Article note: Published in the special issue Biophotonics with Guest Editors Jurgen Popp and Reiner Salzer.
Raman Spectroscopy ; Microfluidics ; Transferases
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