Inflammation Research, 2013, Vol.62(1), pp.9-15
Byline: Depeng Li (1), Yunhe Fu (1), Wen Zhang (1), Gaoli Su (1), Bo Liu (1), Mengyao Guo (1), Fengyang Li (1), Dejie Liang (1), Zhicheng Liu (1), Xichen Zhang (1), Yongguo Cao (1), Naisheng Zhang (1), Zhengtao Yang (1) Keywords: Salidroside; Lipopolysaccharide (LPS); Mastitis; Cytokine; Nuclear factor-kappaB (NF-IoB); Mitogen activated protein kinases (MAPKs) Abstract: Background and objective Mastitis is defined as inflammation of the mammary gland in domestic dairy animals and humans. Salidroside, a major component isolated from Rhodiola rosea L., has potent anti-inflammatory properties, but whether it can be used in mastitis treatment has not yet been investigated. The aim of this study was to assess the protective effects of salidroside against lipopolysaccharide (LPS)-induced mastitis in mice and the mechanism of action. Methods and results We used a mouse mastitis model in which mammary gland inflammation was induced by LPS challenge. Salidroside administered 1 h before LPS infusion significantly attenuated inflammatory cell infiltration, reduced the activity of myeloperoxidase in mammary tissue, and decreased the concentration of tumor necrosis factor-[alpha], interleukin (IL)-1[beta], and IL-6 in a dose-dependent manner. Further studies revealed that salidroside down-regulated phosphorylation of LPS-induced nuclear transcription factor-kappaB (NF-IoB) p65 and inhibitor of NF-IoB [alpha] (IIoB[alpha]) in the NF-IoB signal pathway, and suppressed phosphorylation of p38, extracellular signal-regulated kinase (ERK) and c-jun NH.sub.2-terminal kinase (JNK) in MAPKs signal pathways. Conclusions This study demonstrates that salidroside is an effective suppressor of inflammation and may be a candidate for the prophylaxis of mastitis. Author Affiliation: (1) Department of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, 130062, Jilin, People's Republic of China Article History: Registration Date: 07/08/2012 Received Date: 02/04/2012 Accepted Date: 06/08/2012 Online Date: 23/08/2012 Article note: Responsible Editor: Graham Wallace. D. Li and Y. Fu contributed equally to this work.
Salidroside ; Lipopolysaccharide (LPS) ; Mastitis ; Cytokine ; Nuclear factor-kappaB (NF-κB) ; Mitogen activated protein kinases (MAPKs)
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