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Format: 1 online resource (xxvi, 582 pages) : , illustrations.

ISBN: 1-281-75466-8 , 9786611754662 , 0-444-81590-2 , 0-08-085860-0

Series Statement: Journal of chromatography library ; v. 52

Content: Capillary Electrophoresis (CE) has had a very significant impact on the field of analytical chemistry in recent years as the technique is capable of very high resolution separations, requiring only small amounts of samples and reagents. Furthermore, it can be readily adapted to automatic sample handling and real time data processing. Many new methodologies based on CE have been reported. Rapid, reproducible separations of extremely small amounts of chemicals and biochemicals, including peptides, proteins, nucleotides, DNA, enantiomers, carbohydrates, vitamins, inorganic ions, pharmaceuticals a

Note: Cover; Copyright Page; Preface; TOCContents; List of Symbols; Abbreviations; CHChapter 1. Introduction; 1.1 Historical background; 1.2 Different modes of capillary electrophoresis; 1.3 Principles of separation in capillary zone electrophoresis (CZE); 1.4 Comparison with other separation techniques; 1.5 Conclusion; 1.6 References; CHChapter 2. Sample Injection Methods; 2.1 Introduction; 2.2 Electrokinetic injection; 2.3 Hydrodynamic injection; 2.4 Electric sample splitter; 2.5 Split flow syringe injection system; 2.6 Rotary-type injector; 2.7 Freeze plug injection , 2.8 Sampling device with feeder2.9 Microinjectors; 2.10 Optical gating; 2.11 On-column fracture for sample introduction; 2.12 Conclusion; 2.13 References; CHChapter 3. Detection Techniques; 3.1 Introduction; 3.2 UV-visible absorbance detectors; 3.3 Photodiode array and multiwavelength UV detection; 3.4 Fluorescence detection; 3.5 Laser-based thermo-optical and refractive index detectors; 3.6 Electrochemical detection; 3.7 Indirect detection; 3.8 Radioisotope detectors; 3.9 Mass spectrometric detection; 3.10 Conclusion; 3.11 References; CHChapter 4. Column Technology; 4.1 Introduction , 4.2 Coated columns4.3 Columns for capillary gel electrophoresis (CGE); 4.4 Packed columns; 4.5 Capillary electrophoresis on a chip; 4.6 Conclusion; 4.7 References; CHChapter 5. Electrolyte Systems; 5.1 Introduction; 5.2 Micellar electrokinetic chromatography (MEKC); 5.3 Use of inclusion complexes; 5.4 Complexing additives; 5.5 Other types of electrophoretic media; 5.6 Conclusion; 5.7 References; CHChapter 6. Special Systems and Methods; 6.1 Introduction; 6.2 Buffer programming; 6.3 Fraction collection; 6.4 Field effect electroosmosis; 6.5 Systematic optimization schemes , 6.6 Determination of electrophoretic mobilities and diffusion coefficients6.7 Capillary isoelectric focusing (CIEF); 6.8 Capillary isotachophoresis; 6.9 Hyphenated techniques; 6.10 Conclusion; 6.11 References; CHChapter 7. Applications; 7.1 Amino acids; 7.2 Peptides; 7.3 Proteins; 7.4 Nucleic acids; 7.5 Pharmaceuticals and drugs; 7.6 Cells, viruses and bacteria; 7.7 Analysis of body fluids; 7.8 Capillary ion analysis; 7.9 Metal chelates; 7.10 Organic compounds; 7.11 Carbohydrates; 7.12 Food analysis; 7.13 Environmental analysis; 7.14 Polymer and particle analysis; 7.15 Natural products , 7.16 Chiral separation7.17 Separation of geometrical and positional isomers; 7.18 Coal and fuels; 7.19 Textile and dyes; 7.20 Explosives; 7.21 Survey of commercial CE instruments; 7.22 Summary of applications of CE; 7.23 References; CHChapter 8. Recent Advances and Prospect for Growth; 8.1 Recent reviews on CE; 8.2 Advances in injection techniques; 8.3 Novel detection techniques; 8.4 Advances in column technology; 8.5 Progress on electrolyte systems; 8.6 New systems and methods; 8.7 Additional applications based on CE; 8.8 Future trends; 8.9 References; IDXSubject index , English

Additional Edition: ISBN 0-444-89433-0

Language: English