Format:
1 Online-Ressource (442 p)
ISBN:
9783319318264
Content:
Preface -- Contents -- Contributors -- Chapter 1: A Historical Perspective on Cardiovascular Proteomics -- Introduction: The Renaissance of Protein Biochemistry -- A Brief History of Proteomics -- Operator Independence Days in Separation Techniques -- Over a Century of Mass Spectrometry -- Genomic and Proteomic Databases: From Genes to Proteins, and Back -- Proteomics with a Heart -- Historical Crossroads: Pick Your New Technologies Wisely -- What Do We Leave Behind as We Move Forward: Bringing the Fun Back into Science -- References
Content:
Chapter 2: Basic Concepts in Mass Spectrometry and Protein Quantitation -- Introduction to Mass Spectrometry -- How a Mass Spectrometer Works -- Methods of Ionization -- Methods of Fragmentation -- Orbitrap MS -- Time-of-Flight MS -- SRM-MS with Triple Quadrupole MS -- DDA and DIA (SWATH) -- Methods for Protein Quantitation -- Spectral Counting -- MS1 Peak Integration -- Stable Isotope Labeling by Amino Acids (SILAC) -- Methods of Labeling Protein Quantitation -- Gel-Based Comparisons -- Isotope-Coded Affinity Tag (ICAT) Labeling -- Isobaric Labeling
Content:
Application of MS Technologies to Quantitation in Cardiac Proteomics -- Concluding Remarks -- References -- Chapter 3: How to Design a Cardiovascular Proteomics Experiment -- Introduction -- Overall Considerations -- What Type of Data Do You Want to Obtain? -- Qualitative or Quantitative -- Protein-Protein Interactions -- PTMs -- Your Biological Question Will Define Your Approach -- Untargeted Approaches -- 2-DE Gels -- Gel-Free -- Label-Free -- Stable Isotope -- Targeted Approaches -- Absolute QUAntification (AQUA) -- Single Reaction Monitoring (SRM) or Multiple-Reaction Monitoring (MRM)
Content:
Limitations and Pitfalls to Consider -- Study Design (Fig. 3.2) -- Before Starting -- Sample Extraction -- Protein Preparation/Separation Technique (Gel-Free and Gel-Based) -- Protein Fractionation (Directly Proportional to Sample Complexity) -- Mass Spectrometers -- Protein Identification -- Data Interpretation, Quantification, and Analysis -- Tweaks and Tricks -- Improved Methods of Digestion for Poorly Solubilized Proteins -- High Throughput Proteomics -- Interacting with a MS Core: How to Achieve Success -- Concluding Remarks -- References
Content:
Chapter 4: Organelle, Protein and Peptide Fractionation in Cardiovascular Proteomics -- Introduction -- Sample Homogenization -- Separation of Particles -- Why Do We Separate Organelles, Proteins and Peptides? -- First Principles of Particle Separation -- Directed Movement of Particles -- Diffusion: Undirected Particle Movement and Its Effect on Separation -- Centrifugation: The Workhorse of Organellar Separation -- Fractionation by Centrifugation -- Differential Velocity Centrifugation -- Density-Gradient Centrifugation -- Rate Zonal Gradient Density Centrifugation
Content:
Isopycnic (Equilibrium) Gradient Density Centrifugation
Note:
Description based upon print version of record
Additional Edition:
9783319318288
Additional Edition:
9783319318264
Additional Edition:
Print version Agnetti, Giulio Manual of Cardiovascular Proteomics Cham : Springer International Publishing,c2016 9783319318264
Language:
English
Keywords:
Electronic books
URL:
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