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  • 1
    Article
    Article
    Julius Kühn-Institut, Bundesforschungsinstitut für Kulturpflanzen
    Language: German
    Keywords: Agriculture;
    ISSN: 18689892
    E-ISSN: 18689892
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  • 2
    Language: English
    In: Food Chemistry, 15 April 2015, Vol.173, pp.321-331
    Description: commonly known as black salsify is a perennial herbaceous plant belonging to the Asteraceae family. The subaerial parts of black salsify are cultivated in Central and Southern Europe as a vegetable. There are only few reports on the chemical composition of . The aim of the present study was to determine the chemical composition of subaerial and aerial parts of this species. In total, twelve compounds were isolated and identified with spectroscopic methods, including a series of seven rare sesquiterpenoids – five bisabolane and two curcumenal derivatives. Furthermore, a fully validated HPLC–DAD–CAD method for the quantification of phenolic compounds of was developed. The cytotoxicities of lignan [(−)-syringaresinol ( )] and bisabolane derivatives from against several cancer cell lines were studied. (−)-Syringaresinol was the only compound active against myeloma cell lines and was also active against colon cancer cell lines. However, this lignan was also found to be cytotoxic for normal peripheral blood mononuclear cells (PBMCs). Two bisabolane derivatives were active against colon cancer cell lines and may be interesting as lead structures for the development of new anti-colon cancer agents.
    Keywords: Scorzonera Hispanica ; Asteraceae ; Polyphenols ; Sesquiterpenoids ; Cytotoxicity ; Chemistry ; Diet & Clinical Nutrition ; Economics
    ISSN: 0308-8146
    E-ISSN: 1873-7072
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  • 3
    Language: English
    In: Journal of agricultural and food chemistry, 24 March 2010, Vol.58(6), pp.3813-9
    Description: The total phenolic and flavonoid contents and 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reduction antioxidant power (FRAP) antioxidant capacities of 19 accessions of Salvia officinalis from the sage collection of the genebank in Gatersleben (Germany) were evaluated. The major phenolic compounds of sage, that is, rosmarinic acid, caffeic acid, carnosol, and carnosic acid, were quantified by reverse-phase high-performance liquid chromatography. The aerial parts of different individual plants of each accession were collected in two consecutive years from the same experimental field at the beginning of their flowering period. The results demonstrated a high variability between accessions. A general decreasing tendency from 2007 to 2008 was observed in most of the estimated parameters, that is, total phenolic, total flavonoid, rosmarinic acid, and caffeic acid contents and DPPH antioxidant activity. A slight opposite trend was obtained with the FRAP antioxidant capacity. Low but variable quantities of carnosol and carnosic acid were evaluated in the sample extracts. Individual plants within accessions were identified with high phenolic content and strong antioxidant activity. The rosmarinic acid content showed up to 8-fold differences between the lowest and the highest values. Overall, the study demonstrated a high variability in secondary metabolites present in sage, which could be used for breeding of highly antioxidative genotypes of S. officinalis.
    Keywords: Antioxidants -- Analysis ; Cinnamates -- Analysis ; Depsides -- Analysis ; Plant Extracts -- Analysis ; Salvia Officinalis -- Chemistry
    ISSN: 00218561
    E-ISSN: 1520-5118
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  • 4
    In: PLoS ONE, 2016, Vol.11(5)
    Description: In a number of crop species hybrids are able to outperform line varieties. The anthers of the autogamous bread wheat plant are normally extruded post anthesis, a trait which is unfavourable for the production of F1 hybrid grain. Higher anther extrusion (AE) promotes cross fertilization for more efficient hybrid seed production. Therefore, this study aimed at the genetic dissection of AE by genome wide association studies (GWAS) and determination of the main effect QTL. We applied GWAS approach to identify DArT markers potentially linked to AE to unfold its genetic basis in a panel of spring wheat accessions. Phenotypic data were collected for three years and best linear unbiased estimate (BLUE) values were calculated across all years. The extent of the AE correlation between growing years and BLUE values ranged from r = +0.56 (2013 vs 2015) to 0.91 (2014 vs BLUE values). The broad sense heritability was 0.84 across all years. Six accessions displayed stable AE 〉80% across all the years. Genotyping data included 2,575 DArT markers (with minimum of 0.05 minor allele frequency applied). AE was influenced both by genotype and by the growing environment. In all, 131 significant marker trait associations (MTAs) (|log 10 ( P )| 〉FDR) were established for AE. AE behaved as a quantitative trait, with five consistently significant markers (significant across at least two years with a significant BLUE value) contributing a minor to modest proportion (4.29% to 8.61%) of the phenotypic variance and affecting the trait either positively or negatively. For this reason, there is potential for breeding for improved AE by gene pyramiding. The consistently significant markers linked to AE could be helpful for marker assisted selection to transfer AE to high yielding varieties allowing to promote the exploitation of hybrid-heterosis in the key crop wheat.
    Keywords: Research Article ; Biology And Life Sciences ; Biology And Life Sciences ; Biology And Life Sciences ; Biology And Life Sciences ; Biology And Life Sciences ; Research And Analysis Methods ; Biology And Life Sciences ; Biology And Life Sciences ; Biology And Life Sciences ; Biology And Life Sciences ; Research And Analysis Methods
    E-ISSN: 1932-6203
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  • 5
    Language: English
    In: Food Chemistry, April 15, 2015, Vol.173, p.321(11)
    Description: To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.foodchem.2014.10.006 Byline: Sebastian Granica, Ulrike Lohwasser, Karin Johrer, Christian Zidorn Abstract: * Isolation of sesquiterpenoids from roots of Scor z o nera hispanica is described. * Validated method for identification and quantification of polyphenols is presented. * Cytotoxicity of isolated compounds against cancer cell lines is studied. Article History: Received 18 June 2014; Revised 23 September 2014; Accepted 2 October 2014
    Keywords: Polyphenols -- Analysis ; Plant Metabolites -- Analysis
    ISSN: 0308-8146
    Source: Cengage Learning, Inc.
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  • 6
    Language: English
    In: PLoS ONE, 01 January 2016, Vol.11(8), p.e0160745
    Description: Informed collecting, conservation, monitoring and utilization of genetic diversity requires knowledge of the distribution and structure of the variation occurring in a species. Hordeum vulgare subsp. spontaneum (K. Koch) Thell., a primary wild...
    Keywords: Sciences (General)
    E-ISSN: 1932-6203
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  • 7
    Language: German
    In: Julius-Kühn-Archiv, 01 July 2016, Issue 453, pp.53-55
    Description: Taxonomy of plant genetic resources is an important input in characterising and evaluating cultivated plants and essential for identification and documentation of the diversity of genebank collections. In former times taxonomical determination was based only on morphological characters. Nowadays,...
    Keywords: Parsley ; Plant Genetic Resources ; Opium Poppy ; Taxonomy ; Agriculture
    ISSN: 1868-9892
    ISSN: 2199921X
    E-ISSN: 1868-9892
    Source: Directory of Open Access Journals (DOAJ)
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  • 8
    Language: English
    In: Molecules, 01 January 2018, Vol.23(2), p.294
    Description: Phytochemical characters were evaluated in a five-year-old lemon balm collection consisting of 15 and 13 subspecies officinalis and altissima accessions, respectively. Stems were lower in essential oil than leaves. First cut leaves (June) gave more oil than those of the second cut (August)....
    Keywords: Lamiaceae ; Melissa Officinalis ; Lemon Balm ; Essential Oil ; Polyphenols ; Rosmarinic Acid ; Chemistry
    E-ISSN: 1420-3049
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  • 9
    Language: English
    In: Biochemical Systematics and Ecology, 2010, Vol.38(2), pp.178-186
    Description: The genetic structure of 19 accessions of from the genebank Gatersleben (Germany) was analyzed with SNP and SSR markers developed from EST sequences of . Analyzes were performed exclusively with HRM (high resolution melt analysis), the alleles were identified applying a beforehand developed strategy for codominant markers. Nine polymorphic markers (PIC between 0.12 and 0.61) were used for a complete allele scan in the sample set of 190 individuals from 19 accessions. AMOVA stated 51% of variance between the populations and 49% within. Principal component analysis showed a main bulk of closely related accessions, which have substantial heterogeneity within themselves. However, some populations, especially two from Romania, were clearly distant. This is in accordance to phytochemical results of the same sample set, where the Romanian populations were distant due to an exotic chemotype of the essential oil. Cluster analysis revealed connections between groups of accessions in detail.
    Keywords: Salvia Officinalis L ; Garden Sage ; High Resolution Melting ; Hrm ; Chemistry ; Ecology
    ISSN: 0305-1978
    E-ISSN: 1873-2925
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  • 10
    Language: English
    In: Euphytica, 2017, Vol.213(1), pp.1-16
    Description: Septoria tritici blotch (STB), caused by the ascomycete fungus Zymoseptoria tritici (also known as Mycosphaerella graminicola ), is one of the most devastating foliar wheat diseases worldwide. Host resistance is the most effective strategy for management of the disease. A factor that complicates the determination of resistance is its reported interaction with heading date (Hd) and plant height (Ph). In this study, we report findings from a genome-wide association study of resistance to STB in a world-wide collection of 96 wheat accessions. The collection was evaluated under conditions of artificial infection for seedling and adult plant STB resistance, Hd and Ph in field trials. Marker-trait associations (MTAs) were detected using a mixed linear model. STB disease severities showed significant phenotypic variation. In total, 73 MTAs involving STB resistance were detected. The chromosomal locations of some of them were similar to known Stb genes or quantitative trait loci; whereas others were detected in new genomic regions. The field experiment showed evidence of genetic association between STB resistance and Hd, but only for a few genotypes. This was corroborated at the molecular level, where a total of eight genomic regions associated with STB resistance were located in similar positions to MTAs for Hd. New genomic regions associated with STB resistance found here could be useful in wheat breeding aimed at controlling STB after validation in relevant genetic backgrounds
    Keywords: DArT ; Triticum aestivum ; Zymoseptoria tritici
    ISSN: 0014-2336
    E-ISSN: 1573-5060
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