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  • 1
    Language: English
    In: Journal of Pain and Symptom Management, February 2015, Vol.49(2), pp.440-441
    Description: To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.jpainsymman.2014.11.243 Byline: Sam Maiser Author Affiliation: Medical College of Wisconsin, Milwaukee, WI
    Keywords: Medicine
    ISSN: 0885-3924
    E-ISSN: 1873-6513
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  • 2
    In: Biotechnology and Bioengineering, January 2014, Vol.111(1), pp.104-114
    Description: In order to define optimal reaction conditions for a random PEGylation process, the influence of process parameters on kinetics and the isoform formation were evaluated. For this, high sensitive isoform analytics combined with automated robotic screenings were applied, representing a classical QbD approach.
    Keywords: Pegylation ; Lysozyme ; High Throughput Process Development ; High Throughput Experimentation ; Quality‐By‐Design
    ISSN: 0006-3592
    E-ISSN: 1097-0290
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  • 3
    In: Neurology, 2013, Vol.80(12), pp.e127-e129
    Description: Resident Dr. A is called to speak with the family of a patient in the neurointensive care unit who has malignant left middle cerebral artery syndrome and is close to brain death. Resident Dr. A has many competing obligations that day, but realizes how important the discussion will be. As he stands outside the family conference room where anxious loved ones wait, he contemplates what he is going to say and how. Having done this before, he feels confident that he can do it well. What he does not realize is that on the other side of the door are 20 frightened family members who speak a different language, have many different beliefs, and have no idea that their family member is dying. Resident Dr. A's confidence is based on sufficient knowledge of the patient's illness and prognosis, but despite decades of formal education, he was never taught how to effectively communicate with patients and their families in this situation.
    Keywords: Conferences ; Prognosis ; Communication ; Brain ; Language ; Neurology & Neuropathology;
    ISSN: 0028-3878
    E-ISSN: 1526632X
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  • 4
    Language: English
    In: Journal of Chromatography A, Dec 14, 2012, Vol.1268, p.102(7)
    Description: To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.chroma.2012.10.047 Byline: Benjamin Maiser (a), Frieder Kroner (a), Florian Dismer (a), Gerald Brenner-Wei[sz] (b), Jurgen Hubbuch (a) Abstract: a* High resolution separation of PEG-lysozyme isoforms using pH gradients. a* Significant increase in resolution compared to salt gradient runs demonstrated. a* In silico prediction and MALDI-MS validation of isoform identification. Author Affiliation: (a) Institute of Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology (KIT), 76131 Karlsruhe, Germany (b) Institute of Functional Interfaces, Karlsruhe Institute of Technology (KIT), 76344 Eggenstein-Leopoldshafen, Germany Article History: Received 7 August 2012; Revised 11 October 2012; Accepted 17 October 2012
    Keywords: Chromatography -- Chemical Properties ; Ph -- Chemical Properties ; Lysozyme -- Chemical Properties
    ISSN: 0021-9673
    Source: Cengage Learning, Inc.
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  • 5
    Language: English
    In: Journal of Chromatography A, Sept 28, 2012, Vol.1257, p.41(7)
    Description: To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.chroma.2012.07.089 Byline: Sigrid K. Hansen, Benjamin Maiser, Jurgen Hubbuch Abstract: a* High throughput analytics approach for protein PEGamers successfully demonstrated. a* Low analysis time for SEC based quantification of protein PEGamers of 2min. a* Multivariate calibration compensates resolution sacrificed to gain analytical speed. Author Affiliation: Institute of Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology (KIT), 76131 Karlsruhe, Germany Article History: Received 19 April 2012; Revised 23 July 2012; Accepted 24 July 2012
    Keywords: Glycols (Class of compounds) -- Analysis ; Chromatography -- Analysis
    ISSN: 0021-9673
    Source: Cengage Learning, Inc.
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  • 6
    Language: English
    In: Journal of Pain and Symptom Management, April 2016, Vol.51(4), pp.789-793
    Description: Locked-in syndrome (LIS) is a rare neurologic disorder rendering an individual quadriplegic and anarthric with preserved self-awareness and normal if not near-normal cognition. A lesion to the ventral pons causes the classic form of LIS, and patients can typically interact with their environment with eye/eyelid movements. LIS patients may live for years with preserved quality of life (QoL) and cognitive function, but with severe disability. However, medical providers and family often underestimate the patient's QoL, and choose less aggressive care. Prompt assessment of decisionality in LIS patients is challenging, but it must be done to allow these patients to participate in their care. We present the case of a 54-year-old man with LIS. The medical team recommended comfort measures, but the family advocated involving the patient in goals of care discussions. The patient was determined to be decisional during the acute hospitalization, and he elected for life-prolonging care. This case emphasizes the importance of unbiased shared decision making, but also the importance of utilizing a practical framework to assess the decision-making capacity in these patients. We provide a suggested approach to determining decision-making capacity in similar cases or conditions.
    Keywords: Stroke ; Decision-Making Capacity ; Ethics ; Surrogate ; Neuropsychology ; Medicine
    ISSN: 0885-3924
    E-ISSN: 1873-6513
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  • 7
    Language: English
    In: Biochemical and Biophysical Research Communications, 23 March 2012, Vol.419(4), pp.698-702
    Description: ► Characterization of the proliferating cell nuclear antigen in (TbPCNA). ► TbPCNA is a suitable marker to detect replication in . ► TbPCNA distribution and regulation is different compared to closely related parasites and . As in most eukaryotic cells, replication is regulated by a conserved group of proteins in the early-diverged parasite . Only a few components of the replication machinery have been described in this parasite and regulation, sub-nuclear localization and timing of replication are not well understood. We characterized the proliferating cell nuclear antigen in (TbPCNA) to establish a spatial and temporal marker for replication. Interestingly, PCNA distribution and regulation is different compared to the closely related parasites and . TbPCNA foci are clearly detectable during S phase of the cell cycle but in contrast to they are not preferentially located at the nuclear periphery. Furthermore, PCNA seems to be degraded when cells enter G2 phase in suggesting different modes of replication regulation or functions of PCNA in these closely related eukaryotes.
    Keywords: Pcna ; Replication ; S Phase Marker ; Cell Cycle Regulation ; Trypanosoma Brucei ; Biology ; Chemistry ; Anatomy & Physiology
    ISSN: 0006-291X
    E-ISSN: 1090-2104
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  • 8
    In: Physical Chemistry Chemical Physics, 2011, Vol.13(14), pp.6699-6709
    Description: Modern fluorescence microscopy applications go along with increasing demands for the employed fluorescent dyes. In this work, we compared antifading formulae utilizing a recently developed reducing and oxidizing system (ROXS) with commercial antifading agents. To systematically test fluorophore performance in fluorescence imaging of biological samples, we carried out photobleaching experiments using fixed cells labeled with various commonly used organic dyes, such as Alexa 488, Alexa 594, Alexa 647, Cy3B, ATTO 550, and ATTO 647N. Quantitative evaluation of (i) photostability, (ii) brightness, and (iii) storage stability of fluorophores in samples mounted in different antifades (AFs) reveal optimal combinations of dyes and AFs. Based on these results we provide guidance on which AF should preferably be used with a specific dye. Finally, we studied the antifading mechanisms of the commercial AFs using single-molecule spectroscopy and reveal that these empirically selected AFs exhibit similar properties to ROXS AFs.
    Keywords: Chemistry;
    ISSN: 1463-9076
    E-ISSN: 1463-9084
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  • 9
    Language: English
    In: Journal of Chromatography A, 28 September 2012, Vol.1257, pp.41-47
    Description: ► High throughput analytics approach for protein PEGamers successfully demonstrated. ► Low analysis time for SEC based quantification of protein PEGamers of 2 min. ► Multivariate calibration compensates resolution sacrificed to gain analytical speed. Size exclusion chromatography (SEC) is often applied for characterization of protein–polyethylene glycol (PEG) conjugates regarding the number of attached PEG chains (PEGamers). SEC analysis is advantageous as it is precise, robust, and straightforward to establish. However, most SEC based assays have a maximal throughput of a few samples per hour. We present a strategy to increase analytical throughput based on combining a short column with a fast flow rate, and finally multivariate calibration in order to compensate for the resolution lost in the trade off for speed. Different multivariate approaches were compared and multilinear regression was shown to result in the most precise calibrations. Further, a dynamic calibration approach was developed in order to account for changes in column performance over time. In this way, it was possible to establish a highly precise assay for protein PEGamer quantification with a throughput of 30 samples per hour.
    Keywords: Multivariate Calibration ; High Throughput Analytics ; High Throughput Experimentation ; Pegamer Quantification ; Protein Pegylation ; High Throughput Process Development ; Chemistry
    ISSN: 0021-9673
    E-ISSN: 18733778
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  • 10
    Language: English
    In: Journal of chromatography, 2012, Vol.1257, pp.41-47
    Description: Size exclusion chromatography (SEC) is often applied for characterization of protein–polyethylene glycol (PEG) conjugates regarding the number of attached PEG chains (PEGamers). SEC analysis is advantageous as it is precise, robust, and straightforward to establish. However, most SEC based assays have a maximal throughput of a few samples per hour. We present a strategy to increase analytical throughput based on combining a short column with a fast flow rate, and finally multivariate calibration in order to compensate for the resolution lost in the trade off for speed. Different multivariate approaches were compared and multilinear regression was shown to result in the most precise calibrations. Further, a dynamic calibration approach was developed in order to account for changes in column performance over time. In this way, it was possible to establish a highly precise assay for protein PEGamer quantification with a throughput of 30 samples per hour. ; p. 41-47.
    Keywords: Gel Chromatography ; Polyethylene Glycol ; Equipment Performance ; Proteins
    ISSN: 0021-9673
    Source: AGRIS (Food and Agriculture Organization of the United Nations)
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