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  • 1
    In: Izvestiia, May  20, 2005, Issue 83, p.20
    Source: East View Information Services
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  • 2
    Language: English
    In: PLoS ONE, 2011, Vol.6(5), p.e19121
    Description: Chemotherapy of glioblastoma is largely ineffective as the blood-brain barrier (BBB) prevents entry of most anticancer agents into the brain. For an efficient treatment of glioblastomas it is necessary to deliver anti-cancer drugs across the intact BBB. Poly(lactic-co-glycolic acid) (PLGA) nanoparticles coated with poloxamer 188 hold great promise as drug carriers for brain delivery after their intravenous injection. In the present study the anti-tumour efficacy of the surfactant-coated doxorubicin-loaded PLGA nanoparticles against rat glioblastoma 101/8 was investigated using histological and immunohistochemical methods. ; The particles were prepared by a high-pressure solvent evaporation technique using 1% polyvinylalcohol (PLGA/PVA) or human serum albumin (PLGA/HSA) as stabilizers. Additionally, lecithin-containing PLGA/HSA particles (Dox-Lecithin-PLGA/HSA) were prepared. For evaluation of the antitumour efficacy the glioblastoma-bearing rats were treated intravenously with the doxorubicin-loaded nanoparticles coated with poloxamer 188 using the following treatment regimen: 3×2.5 mg/kg on day 2, 5 and 8 after tumour implantation; doxorubicin and poloxamer 188 solutions were used as controls. On day 18, the rats were sacrificed and the antitumour effect was determined by measurement of tumour size, necrotic areas, proliferation index, and expression of GFAP and VEGF as well as Isolectin B4, a marker for the vessel density. ; The results reveal a considerable anti-tumour effect of the doxorubicin-loaded nanoparticles. The overall best results were observed for Dox-Lecithin-PLGA/HSA. These data demonstrate that the poloxamer 188-coated PLGA nanoparticles enable delivery of doxorubicin across the blood-brain barrier in the therapeutically effective concentrations.
    Keywords: Research Article ; Biology ; Medicine ; Immunology ; Oncology
    E-ISSN: 1932-6203
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  • 3
    Language: Russian
    In: Bulletin of the Moscow State Regional University (Linguistics), 2016, Issue 2, pp.111-116
    ISSN: 2310-712X
    Source: CrossRef
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  • 4
    Language: English
    In: International Journal of Pharmaceutics, 30 May 2017, Vol.524(1-2), pp.77-90
    Description: The paramount problem in the therapy of brain tumors is the inability of most drugs to cross the blood–brain barrier. PLGA nanoparticles overcoated with poloxamer 188 could overcome this problem and enabled a high anti-tumoral effect against the very aggressive intracranial 101.8 glioblastoma in rats that closely resembles human grade IV glioblastomas. The basis for the transport of these particles across the blood–brain barrier appears to be adsorption of blood apolipoproteins (ApoE or ApoA-I) on the nanoparticle surface caused by the poloxamer 188-coating, followed by receptor-mediated transcytosis of the nanoparticles. The objective of the present study is the elucidation of the mechanism by which the poloxamer 188-coated nanoparticles then enter the brain tumor cells. Their intracellular fate, therefore, was investigated using the U87 human glioma cell line. The main mechanism of the PLGA nanoparticle internalization by U87 cells was clathrin-mediated endocytosis. Within 1 h free doxorubicin was released from late endosomes and could reach its target site, i.e. the DNA in the nuclei without degradation, whereas the PLGA nanoparticles, which were labeled with Cy5.5, still were observed in the endo-lysosomal compartment. These results demonstrate that the underlying mechanism of action in the brain cells is by diffusive doxorubicin release from the nanoparticles rather than by their intracellular degradation.
    Keywords: Doxorubicin ; Endocytosis ; Plga Nanoparticles ; Poloxamer 188 ; U87 Human Glioma Cell Line ; Pharmacy, Therapeutics, & Pharmacology
    ISSN: 0378-5173
    E-ISSN: 1873-3476
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  • 5
    Language: English
    In: International Journal of Pharmaceutics, 25 August 2018, Vol.547(1-2), pp.10-23
    Description: Resistance to antiepileptic drugs (AEDs) is a major clinical problem. The overexpression of P-glycoprotein (Pgp), one of the main transporters limiting the entry of xenobiotics into the brain, is among the factors contributing to the AED resistance. Presently, there is no consensus on the interaction of carbamazepine (CBZ) with the Pgp. This study investigates the effect of the Pgp inhibitor verapamil on the anticonvulsant effect of CBZ and its nanoparticulate formulation in the rat model of isoniazid-induced epilepsy. Verapamil significantly increased the anticonvulsant effect of CBZ and reduced its effective dose by at least 30% (from 30 mg/kg to 20 mg/kg). Binding of carbamazepine to the poloxamer 188-coated PLGA nanoparticles enabled a 30-fold increase of its anticonvulsive effect, as compared to the free drug. The inhibition of Pgp did not influence the effectivity of carbamazepine encapsulated in nanoparticles.
    Keywords: Blood-Brain Barrier ; Carbamazepine ; Epilepsy ; Plga Nanoparticles ; Poloxamer 188 ; Verapamil ; Pharmacy, Therapeutics, & Pharmacology
    ISSN: 0378-5173
    E-ISSN: 1873-3476
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  • 6
    Language: English
    In: Journal of Controlled Release, 10 May 2017, Vol.253, pp.1-10
    Description: The lysosomal storage disorder (LSD) metachromatic leukodystrophy (MLD) is caused by a deficiency of the soluble, lysosomal hydrolase arylsulfatase A (ASA). The disease is characterized by accumulation of 3-O-sulfogalactosylceramide (sulfatide), progressive demyelination of the nervous system and premature death. Enzyme replacement therapy (ERT), based on regular intravenous injections of recombinant functional enzyme, is in clinical use for several LSDs. For MLD and other LSDs with central nervous system (CNS) involvement, however, ERT is limited by the blood-brain barrier (BBB) restricting transport of therapeutic enzymes from the blood to the brain. In the present study, the potential of different types of surfactant-coated biodegradable nanoparticles to increase brain delivery of ASA was evaluated. Three different strategies to bind ASA to nanoparticle surfaces were compared: (1) adsorption, (2) high-affinity binding via the streptavidin-biotin system, and (3) covalent binding. Adsorption allowed binding of high amounts of active ASA. However, in presence of phosphate-buffered saline or serum rapid and complete desorption occurred, rendering this strategy ineffective for in vivo applications. In contrast, stable immobilization with negligible dissociation was achieved by high-affinity and covalent binding. Consequently, we analyzed the brain targeting of two stably nanoparticle-bound ASA formulations in ASA mice, an animal model of MLD. Compared to free ASA, injected as a control, the biodistribution of nanoparticle-bound ASA was altered in peripheral organs, but no increase of brain levels was detectable. The failure to improve brain delivery suggests that the ASA glycoprotein interferes with processes required to target surfactant-coated nanoparticles to brain capillary endothelial cells.
    Keywords: Metachromatic Leukodystrophy ; Lysosomal Storage Disease ; Enzyme Replacement Therapy ; Blood-Brain Barrier ; Polymeric Nanoparticles ; Surfactant Coating ; Pharmacy, Therapeutics, & Pharmacology
    ISSN: 0168-3659
    E-ISSN: 1873-4995
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  • 7
    Language: Russian
    In: Bulletin of the Moscow State Regional University (Linguistics), 2016, Issue 4, pp.27-37
    ISSN: 2310-712X
    Source: CrossRef
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  • 8
    Language: Russian
    In: Вестник Московского государственного областного университета, 01 September 2018, Issue 3, pp.262-269
    Description: The purpose of the present article is to analyze the functions of the Latin language on the Internet both as a cross-cultural communication phenomenon and as the subject of linguodidactics. The analysis of tendencies aimed at reviving Latin in online and offline modes is provided. Means of Latin application on the Internet as, for example, in broadcasting, linguistic educational programs, mass media, digital informational resources, etc. are considered. Methods of the research are as follows: collection and differential selection, systematization, classification. The result of the research performed is the classification of methods of Latin functioning on the Internet which demonstrates that Latin is gradually changing its status of the dead language and is becoming rather popular on the network. The results of the research contribute to the Internet discourse theory and linguistic part of the Internet discourse on the example of Latin. An assumption of a possible change of the status of Latin due to the Internet is considered.
    Keywords: Internet ; Latin ; Mass-Media ; Congresses ; Computer Linguistic Systems ; Sciences (General)
    ISSN: Bulletin of the Moscow State Regional University
    E-ISSN: 2224-0209
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  • 9
    In: Izvestiia, May  20, 2005, Issue 83, p.20
    Source: East View Information Services
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  • 10
    Article
    Article
    In: Izvestiia, August  05, 2005, Issue 136, p.12
    Source: East View Information Services
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