Biosensors and Bioelectronics, May 15, 2014, Vol.55, p.44(7)
To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.bios.2013.11.060 Byline: Rangadhar Pradhan, Shashi Rajput, Mahitosh Mandal, Analava Mitra, Soumen Das Abstract: The present work reports the impedance characteristics of MCF-7 cell lines treated with anticancer drug ZD6474 to evaluate the cytotoxic effect on cellular electrical behaviour using miniature impedance sensors. Four types of impedance sensing devices with different electrode geometries are fabricated by microfabrication technology. The frequency response characteristics of drug treated cells are studied to evaluate cytotoxic effect of ZD6474 and also to assess the frequency dependent sensitivity variation with electrode area. A significant variation in magnitude of measured impedance data is obtained for drug treated samples above 10A[micro]M dose indicating prominent effect of ZD6474 which results in suppression of cell proliferation and induction of apoptosis process. The results obtained by impedimetric method are correlated well with conventional in vitro assays such as flow cytometry, cell viability assays and microscopic imaging. Finally an empirical relation between cell impedance, electrode area and drug dose is established from impedance data which exhibits a negative correlation between drug doses and impedance of cancer cells. Author Affiliation: School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur 721302, India Article History: Received 21 August 2013; Revised 8 November 2013; Accepted 20 November 2013
Breast Cancer -- Drug Therapy ; Breast Cancer -- Electric Properties ; Antineoplastic Agents -- Electric Properties
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