Brain Research, June 17, 2013, Vol.1515, p.29(10)
To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.brainres.2013.03.032 Byline: Lawrence S. Own, Rimsha Iqbal, Paresh D. Patel Abstract: Adverse early life experiences (aELEs), such as child abuse, neglect, or trauma, increase lifetime vulnerability for mental illness. In this study, aELEs were modeled in c57bl/6 mice using the maternal separation (MS) paradigm, in which pups were separated for 180min/day (MS180), 15min/day (MS15), or left undisturbed (AFR) from postnatal day 2-14. As adults, pups that experienced MS15 or MS180 demonstrated decreases in tryptophan hydroxylase 2 and serotonin transporter mRNA in the dorsal raphe dorsalis and ventralis, and increases in glucocorticoid receptor mRNA in the dentate gyrus of the hippocampus. To investigate factors underlying shared expression between MS conditions, dam on-nest time and DNA methylation at the TPH2 promoter and 5' UTR were assessed. Post-reunion on-nest time increased as a function of separation duration, potentially serving as a mitigating factor underlying similar expression between MS conditions. TPH2 DNA methylation remained unchanged, suggesting changes in TPH2 mRNA are not mediated by changes in DNA methylation of this region. The shared pattern of expression between MS15 and MS180 conditions suggests a species- or strain- specific response to MS unique to c57bl/6 mice. Author Affiliation: (a) Molecular and Behavioral Neuroscience Institute, University of Michigan Medical Center, 5698 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200, USA (b) Department of Psychiatry, University of Michigan Medical Center, 5062 BSRB, 1500 E. Medical Center Drive, Ann Arbor, MI 48109, USA (c) Neuroscience Graduate Program, University of Michigan, 4163 Undergraduate Science Building, Ann Arbor, MI 48109-2200, USA (d) Undergraduate Research Opportunities Program, University of Michigan, 1190 Undergraduate Science Building, Ann Arbor, MI 48109-2200, USA Article History: Accepted 13 March 2013
Serotonin -- Analysis ; House Mouse -- Analysis ; Genetic Research -- Analysis ; Tryptophan -- Analysis ; Mental Disorders -- Analysis ; Methylation -- Analysis ; Neurosciences -- Analysis ; Child Abuse -- Analysis ; Glucocorticoids -- Analysis ; Rna -- Analysis ; Gene Expression -- Analysis ; Hydroxylases -- Analysis
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