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Berlin Brandenburg

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  • 1
    Language: English
    In: International journal of cancer, 01 October 2013, Vol.133(7), pp.1624-30
    Description: The differentiation between hereditary and sporadic microsatellite-unstable (MSI-H) colorectal cancer is a crucial step in Lynch syndrome diagnostics. Within MSI-H colorectal cancers, the BRAF V600E mutation is strongly associated with sporadic origin. Here, we asked whether BRAF V600E-specific immunohistochemistry (clone VE1) is helpful in separating sporadic from Lynch syndrome-associated MSI-H colorectal cancers. To that end, we performed VE1 immunohistochemistry and BRAF sequencing in a series of 91 MSI-H colorectal cancer specimens from patients tested for Lynch syndrome. Concordance of VE1 immunohistochemistry and molecular BRAF mutation status was observed in 90 of 91 (98.9%) MSI-H samples. All 11 tumors classified as BRAF V600E mutation-positive by Sanger sequencing were immunopositive, and 79 (98.8%) of 80 tumors classified as BRAF wild type showed negative staining. All VE1-positive tumors were MLH1- and PMS2-negative by immunohistochemistry. None of the tumors from mismatch repair (MMR) gene germline mutation carriers (n = 28) displayed positive VE1 staining, indicating that BRAF V600E mutation-specific immunostaining has a low risk of excluding Lynch syndrome patients from germline mutation analysis. In conclusion, implementation of VE1 immunohistochemistry was able to detect BRAF-mutated MSI-H colorectal cancers with a sensitivity of 100% and a specificity of 98.8%. Among MLH1-negative colorectal cancers, the rate of VE1-positive lesions was 21%, offering the exclusion of these patients from MMR germline testing. Therefore, we suggest the integration of VE1 immunohistochemistry into the diagnostic panel of Lynch syndrome.
    Keywords: Braf V600e ; Lynch Syndrome ; Colorectal Cancer ; Hereditary Nonpolyposis Colorectal Cancer ; Microsatellite Instability ; Microsatellite Instability ; Adaptor Proteins, Signal Transducing -- Genetics ; Colorectal Neoplasms, Hereditary Nonpolyposis -- Diagnosis ; Nuclear Proteins -- Genetics ; Proto-Oncogene Proteins B-Raf -- Genetics
    ISSN: 00207136
    E-ISSN: 1097-0215
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  • 2
    Language: English
    In: PLoS ONE, 01 January 2014, Vol.9(11), p.e110727
    Description: PURPOSE: To analyze if tumor vessels can be visualized, segmented and quantified in glioblastoma patients with time of flight (ToF) angiography at 7 Tesla and multiscale vessel enhancement filtering. MATERIALS AND METHODS: Twelve patients with newly diagnosed glioblastoma were examined with...
    Keywords: Sciences (General)
    E-ISSN: 1932-6203
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  • 3
    Language: English
    In: PLoS ONE, 01 January 2015, Vol.10(3), p.e0121220
    Description: To explore the correlation between Nuclear Overhauser Enhancement (NOE)-mediated signals and tumor cellularity in glioblastoma utilizing the apparent diffusion coefficient (ADC) and cell density from histologic specimens. NOE is one type of chemical exchange saturation transfer (CEST) that...
    Keywords: Sciences (General)
    E-ISSN: 1932-6203
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  • 4
    Language: English
    In: PLoS ONE, 01 January 2015, Vol.10(4), p.e0120801
    Description: Due to its sensitivity to deoxyhemoglobin, susceptibility weighted imaging (SWI) enables the visualization of deep medullary veins (DMV) in patients with acute stroke, which are difficult to depict under physiological circumstances. This study assesses the asymmetric appearance of prominent...
    Keywords: Sciences (General)
    E-ISSN: 1932-6203
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  • 5
    In: PLoS ONE, 2017, Vol.12(3)
    Description: Objective The objective of this prospective study was to evaluate whether magnetic resonance imaging (MRI) is equivalent to lateral cephalometric radiographs (LCR, “gold standard”) in cephalometric analysis. Methods The applied MRI technique was optimized for short scanning time, high resolution, high contrast and geometric accuracy. Prior to orthodontic treatment, 20 patients (mean age ± SD, 13.95 years ± 5.34) received MRI and LCR. MRI datasets were postprocessed into lateral cephalograms. Cephalometric analysis was performed twice by two independent observers for both modalities with an interval of 4 weeks. Eight bilateral and 10 midsagittal landmarks were identified, and 24 widely used measurements (14 angles, 10 distances) were calculated. Statistical analysis was performed by using intraclass correlation coefficient (ICC), Bland-Altman analysis and two one-sided tests (TOST) within the predefined equivalence margin of ± 2°/mm. Results Geometric accuracy of the MRI technique was confirmed by phantom measurements. Mean intraobserver ICC were 0.977/0.975 for MRI and 0.975/0.961 for LCR. Average interobserver ICC were 0.980 for MRI and 0.929 for LCR. Bland-Altman analysis showed high levels of agreement between the two modalities, bias range (mean ± SD) was -0.66 to 0.61 mm (0.06 ± 0.44) for distances and -1.33 to 1.14° (0.06 ± 0.71) for angles. Except for the interincisal angle ( p = 0.17) all measurements were statistically equivalent ( p 〈 0.05). Conclusions This study demonstrates feasibility of orthodontic treatment planning without radiation exposure based on MRI. High-resolution isotropic MRI datasets can be transformed into lateral cephalograms allowing reliable measurements as applied in orthodontic routine with high concordance to the corresponding measurements on LCR.
    Keywords: Research Article ; Medicine And Health Sciences ; Research And Analysis Methods ; Medicine And Health Sciences ; Medicine And Health Sciences ; Biology And Life Sciences ; Medicine And Health Sciences ; Biology And Life Sciences ; Medicine And Health Sciences ; Medicine And Health Sciences ; Biology And Life Sciences ; Medicine And Health Sciences ; Research And Analysis Methods ; Biology And Life Sciences ; Medicine And Health Sciences ; Research And Analysis Methods ; Medicine And Health Sciences ; People And Places ; Research And Analysis Methods
    E-ISSN: 1932-6203
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  • 6
    Language: English
    In: PLoS ONE, 01 January 2017, Vol.12(4), p.e0174620
    Description: The purpose of this study was to investigate whether a voxel-wise analysis of apparent diffusion coefficient (ADC) values may differentiate between progressive disease (PD) and pseudoprogression (PsP) in patients with high-grade glioma using...
    Keywords: Sciences (General)
    E-ISSN: 1932-6203
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  • 7
    In: TumorDiagnostik & Therapie, 2017, Vol.38(03), pp.178-184
    Description: Im vorliegenden Übersichtsartikel werden die wesentlichen Entwicklungen der letzten Jahre der MR-Bildgebung bei Gliomen aufgezeigt. Schwerpunkte sind dabei die sog. RANO-Kriterien (Kriterien für das Radiology Assessment in der Neuroonkologie), die umfassende Änderungen in der Bewertung des Therapieansprechens höhergradiger Gliome mit sich brachten, sowie neue, sog. funktionelle MR-Sequenzen. Beschränkte sich die traditionelle Diagnostik bei höhergradigen Gliomen auf kontrastmittelverstärkte T1w Aufnahmen, so wurden mit Einführung der RANO-Kriterien erstmals auch T2w Sequenzen in die Beurteilung des Therapieansprechens einbezogen. Weiterhin wurde in den letzten Jahren der potenzielle Nutzen funktioneller MR-Sequenzen erforscht, die zum Teil Parameter der Tumorbiologie (z. B. Tumorvaskularisation) unmittelbar darstellen können. Nach einer kurzen Vorstellung der wesentlichen, mit Einführung der RANO-Kriterien einhergehenden Änderungen werden in diesem Übersichtsartikel die in der Praxis geläufigsten funktionellen MR-Sequenzen beschrieben: MR-Diffusion, MR-Perfusion und SWI (suszeptibilitätsgewichtete Bildgebung). Darüber hinaus wird ihr potenzieller klinischer Nutzen diskutiert. Abschließend wird ein Ausblick gegeben auf mögliche zukünftige Entwicklungen der MR-Bildgebung der Gliome. Dabei stehen die Ultrahochfeld-MRT bei 7 T (Tesla) sowie die sog. Radiomics im Zentrum der Ausführungen.
    ISSN: 0722-219X
    E-ISSN: 1439-1279
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  • 8
    Language: German
    In: Neuroradiologie Scan, 2016, Vol.06(04), pp.323-333
    Description: Im vorliegenden Übersichtsartikel werden die wesentlichen Entwicklungen der letzten Jahre der MR-Bildgebung bei Gliomen aufgezeigt. Schwerpunkte sind dabei die sog. RANO-Kriterien (Kriterien für das Radiology Assessment in der Neuroonkologie), die umfassende Änderungen in der Bewertung des Therapieansprechens höhergradiger Gliome mit sich brachten, sowie neue, sog. funktionelle MR-Sequenzen. Beschränkte sich die traditionelle Diagnostik bei höhergradigen Gliomen auf kontrastmittelverstärkte T1w Aufnahmen, so wurden mit Einführung der RANO-Kriterien erstmals auch T2w Sequenzen in die Beurteilung des Therapieansprechens einbezogen. Weiterhin wurde in den letzten Jahren der potenzielle Nutzen funktioneller MR-Sequenzen erforscht, die zum Teil Parameter der Tumorbiologie (z. B. Tumorvaskularisation) unmittelbar darstellen können. Nach einer kurzen Vorstellung der wesentlichen, mit Einführung der RANO-Kriterien einhergehenden Änderungen werden in diesem Übersichtsartikel die in der Praxis geläufigsten funktionellen MR-Sequenzen beschrieben: MR-Diffusion, MR-Perfusion und SWI (suszeptibilitätsgewichtete Bildgebung). Darüber hinaus wird ihr potenzieller klinischer Nutzen diskutiert. Abschließend wird ein Ausblick gegeben auf mögliche zukünftige Entwicklungen der MR-Bildgebung der Gliome. Dabei stehen die Ultrahochfeld-MRT bei 7 T (Tesla) sowie die sog. Radiomics im Zentrum der Ausführungen.
    ISSN: 1616-9697
    E-ISSN: 1616-9700
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  • 9
    In: Seminars in Neurology, 2018, Vol.38(01)
    In: Seminars in Neurology, 2018, Vol.38(01), pp.032-040
    Description: Magnetic resonance imaging plays a key role in diagnosis and treatment monitoring of brain tumors. Novel imaging techniques that specifically interrogate aspects of underlying tumor biology and biochemical pathways have great potential in neuro-oncology. This review focuses on the emerging role of 2-hydroxyglutarate-targeted magnetic resonance spectroscopy, as well as radiomics and radiogenomics in establishing diagnosis for isocitrate dehydrogenase mutant gliomas, and for monitoring treatment response and predicting prognosis of this group of brain tumor patients.
    Keywords: Isocitrate dehydrogenase ; 2-hydroxyglutarate ; Magnetic resonance spectroscopic imaging ; Radiomics ; Radiogenomics
    ISSN: 0271-8235
    E-ISSN: 1098-9021
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  • 10
    Language: English
    In: PLoS ONE, Nov 21, 2014, Vol.9(11)
    Description: Purpose To analyze if tumor vessels can be visualized, segmented and quantified in glioblastoma patients with time of flight (ToF) angiography at 7 Tesla and multiscale vessel enhancement filtering. Materials and Methods Twelve patients with newly diagnosed glioblastoma were examined with ToF angiography (TR = 15 ms, TE = 4.8 ms, flip angle = 15#176;, FOV = 160x210 mm.sup.2, voxel size: 0.31x0.31x0.40 mm.sup.3) on a whole-body 7 T MR system. A volume of interest (VOI) was placed within the border of the contrast enhancing part on T1-weighted images of the glioblastoma and a reference VOI was placed in the non-affected contralateral white matter. Automated segmentation and quantification of vessels within the two VOIs was achieved using multiscale vessel enhancement filtering in ImageJ. Results Tumor vessels were clearly visible in all patients. When comparing tumor and the reference VOI, total vessel surface (45.3#177;13.9 mm.sup.2 vs. 29.0#177;21.0 mm.sup.2 (p0.035)) and number of branches (3.5#177;1.8 vs. 1.0#177;0.6 (p0.001) per cubic centimeter were significantly higher, while mean vessel branch length was significantly lower (3.8#177;1.5 mm vs 7.2#177;2.8 mm (p0.001)) in the tumor. Discussion ToF angiography at 7-Tesla MRI enables characterization and quantification of the internal vascular morphology of glioblastoma and may be used for the evaluation of therapy response within future studies.
    Keywords: Medical Research ; Glioblastomas ; Angiography
    ISSN: 1932-6203
    Source: Cengage Learning, Inc.
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