Kooperativer Bibliotheksverbund

Berlin Brandenburg

and
and

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
Language
Year
  • 1
    In: Neuro-Oncology, 2018, Vol. 20(suppl6), pp.vi45-vi46
    Description: We have previously demonstrated that Myc-overexpressing medulloblastoma cells are sensitive to apoptosis induction in response to mitotic inhibition with an Aurora kinase B inhibitor. Profiling of differential mRNA expression by microarray and RT-PCR revealed that alpha cardiac actin 1 ( ACTC1 ) mRNA expression is upregulated in cells resistant to apoptosis triggered by Aurora kinase B inhibition and this upregulation is absent in Myc-overexpressing cells. We confirmed expression of ACTC1 protein by Western blot in SHH subgroup (DAOY, UW228, UW426) and Group 3 subgroup cell lines (D458, D425) and observed that expression is lower in Group 3 cells. These findings were further validated by analysis of ACTC1 mRNA expression among all four medulloblastoma subgroups by microarray in a set of 763 medulloblastomas which revealed increased expression of ACTC1 in SHH and WNT subgroups compared to Group 3 and Group 4. Inhibition of Aurora kinase B in SHH cells that overexpress Myc results in a reduction in ACTC1 protein level after 96 hours and this is not observed in the isotype control cells with basal Myc expression. Reduction in ACTC1 protein levels in SHH cells overexpressing Myc is associated with Caspase 3 cleavage. ACTC1 may play a role in blocking apoptosis triggered by mitotic inhibition in medulloblastoma. Further experiments to test this hypothesis are planned. These finding could potentially impact on chemotherapy choice in the treatment of SHH and WNT tumours, which demonstrate increased ACTC1 expression.
    Keywords: Medicine;
    ISSN: 1522-8517
    E-ISSN: 1523-5866
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 2
    Language: English
    In: Biophysical Chemistry, Dec 15, 2013, Vol.183, p.30(12)
    Description: To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.bpc.2013.06.019 Byline: Aude Picard, Isabelle Daniel Abstract: Microbial life has been prevailing in the biosphere for the last 3.8Ga at least. Throughout most of the Earth's history it has experienced a range of pressures; both dynamic pressure when the young Earth was heavily bombarded, and static pressure in subsurface environments that could have served as a refuge and where microbial life nowadays flourishes. In this review, we discuss the extent of high-pressure habitats in early and modern times and provide a short overview of microbial survival under dynamic pressures. We summarize the current knowledge about the impact of microbial activity on biogeochemical cycles under pressures characteristic of the deep subsurface. We evaluate the possibility that pressure can be a limiting parameter for life at depth. Finally, we discuss the open questions and knowledge gaps that exist in the field of high-pressure geomicrobiology. Article History: Received 4 April 2013; Revised 18 June 2013; Accepted 22 June 2013
    Keywords: Biogeochemical Cycles -- Environmental Aspects
    ISSN: 0301-4622
    Source: Cengage Learning, Inc.
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 3
    Language: English
    In: Canadian Journal of Public Health, 2001, Vol.92(5), pp.353-358
    Description: Potential determinants of bone mass were investigated in a group of 70 young females (mean age 26.6 years), daughters of women studied in premenopause. Nutritional data, leisure physical activity level, lifestyle habits as well as familial similarities were assessed. The daughters’ bone mineral density (BMD), measured by dual-energy absorp-tiometry, was significantly correlated with their body mass index (BMI) (r = 0.22), dietary vitamin D intake (r = 0.19) and their mothers’ BMD (r = 0.44). Multiple regression analysis indicated that only the mothers’ BMD remained an independent predictor of bone mass. Mother-daughter correlations were also observed for body weight (r = 0.24), height (r = 0.39), BMI (r = 0.29), dietary calcium intake (r = 0.20), and calcium (r = 0.20) or vitamin D (r = 0.25) intakes from dairy products. Hence, these observations support the evidence that mothers’ BMD is the strongest predictor of bone mass of young women in their third decade. Nous avons évalué les principaux déterminants de la masse osseuse chez 70 jeunes femmes (âge moyen: 26,6 ans) dont les mères avaient été étudiées avant la ménopause. Notre évaluation a porté sur les apports nutritionnels, le niveau d’activité physique durant les loisirs, le mode de vie et les antécédents familiaux. Nous avons mesuré la densité osseuse de la colonne lombaire (DO) par densitométrie biphotonique. Afin de déterminer l’influence de l’hérédité et de l’environnement, ces paramètres ont été comparés à ceux des mères. La DO des filles était en relation significative avec celle de leur mère (r = 0,44), l’indice de masse corporelle (IMC) (r = 0,22) et l’apport alimentaire en vitamine D (r = 0,19). Par régression multiple, seule la DO des mères a été associée à la DO des filles. Des corrélations mère-fille ont été observées pour le poids (r = 0,24), la taille (r = 0,39), l’IMC (r = 0,29), l’apport en calcium (r = 0,20) et les apports en calcium (r = 0,20) ou en vitamine D (r = 0,25) provenant des produits laitiers. Ces observations suggèrent que la DO de la mère est le principal déterminant de la masse osseuse chez les jeunes femmes dans la trentaine.
    Keywords: Public Health;
    ISSN: 0008-4263
    E-ISSN: 1920-7476
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 4
    Language: English
    In: Cancer Research, 10/01/2014, Vol.74(19 Supplement), pp.3089-3089
    ISSN: 0008-5472
    E-ISSN: 1538-7445
    Source: CrossRef
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 5
    Language: English
    In: Geobiology, March 2011, Vol.9(2), pp.196-204
    Description: X-ray absorption spectroscopy is a well-established method for probing local structural and electronic atomic environments in a variety of systems. We used X-ray absorption near-edge structure (XANES) spectroscopy for monitoring in real-time conditions selenium reduction in situ in live...
    Keywords: Sciences of the Universe ; Earth Sciences ; Speciation ; Seawater Samples ; Selenium ; Near-Edge Structure ; Ralstonia-Metallidurans Ch34 ; Diamond-Anvil Cell ; Deep-Sea ; Shewanella-Oneidensis ; Gigapascal Pressures ; Hydrostatic-Pressure ; Biology
    ISSN: 1472-4677
    E-ISSN: 1472-4669
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 6
    Language: English
    In: Cancer Research, 04/15/2010, Vol.70(8 Supplement), pp.3109-3109
    ISSN: 0008-5472
    E-ISSN: 1538-7445
    Source: CrossRef
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 7
    Language: English
    In: Annals of the New York Academy of Sciences, Feb, 2010, Vol.1189, p.113(8)
    Description: To authenticate to the full-text of this article, please visit this link: http://dx.doi.org/10.1111/j.1749-6632.2009.05176.x Byline: Phil M. Oger (1), Isabelle Daniel (1), Aude Picard (1) Keywords: diamond anvil cell; high pressure; in situ monitoring; metabolism Abstract: Until recently, monitoring of cells and cellular activities at high hydrostatic pressure (HHP) was mainly limited to ex situ observations. Samples were analyzed prior to and following the depressurization step to evaluate the effect of the pressure treatment. Such ex situ measurements have several drawbacks: (i) it does not allow for kinetic measurements and (ii) the depressurization step often leads to artifactual measurements. Here, we describe recent advances in diamond anvil cell (DAC) technology to adapt it to the monitoring of microbial processes in situ. The modified DAC is asymmetrical, with a single anvil and a diamond window to improve imaging quality and signal collection. Using this novel DAC combined to Raman and X-ray spectroscopy, we monitored the metabolism of glucose by baker's yeast and the reduction of selenite by Agrobacterium tumefaciens in situ under HHP. In situ spectroscopy is also a promising tool to study piezophilic microorganisms. Author Affiliation: (1)Laboratoire de Science de la Terre, Universite de Lyon, Lyon, France Article note: Address for correspondence: Dr. Phil Oger, Laboratoire de Science de la Terre, CNRS UMR 5570, Universite de Lyon, Ecole Normale Superieure de Lyon, 46 allee d'Italie, F-69364 Lyon Cedex 07, France. poger@ens-lyon.fr
    Keywords: X-ray Spectroscopy ; Glucose Metabolism ; Glucose
    ISSN: 0077-8923
    Source: Cengage Learning, Inc.
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 8
    Language: English
    In: Annals of the New York Academy of Sciences, Feb, 2010, Vol.1189, p.113(8)
    Description: To authenticate to the full-text of this article, please visit this link: http://dx.doi.org/10.1111/j.1749-6632.2009.05176.x Byline: Phil M. Oger (1), Isabelle Daniel (1), Aude Picard (1) Keywords: diamond anvil cell; high pressure; in situ monitoring; metabolism Abstract: Until recently, monitoring of cells and cellular activities at high hydrostatic pressure (HHP) was mainly limited to ex situ observations. Samples were analyzed prior to and following the depressurization step to evaluate the effect of the pressure treatment. Such ex situ measurements have several drawbacks: (i) it does not allow for kinetic measurements and (ii) the depressurization step often leads to artifactual measurements. Here, we describe recent advances in diamond anvil cell (DAC) technology to adapt it to the monitoring of microbial processes in situ. The modified DAC is asymmetrical, with a single anvil and a diamond window to improve imaging quality and signal collection. Using this novel DAC combined to Raman and X-ray spectroscopy, we monitored the metabolism of glucose by baker's yeast and the reduction of selenite by Agrobacterium tumefaciens in situ under HHP. In situ spectroscopy is also a promising tool to study piezophilic microorganisms. Author Affiliation: (1)Laboratoire de Science de la Terre, Universite de Lyon, Lyon, France Article note: Address for correspondence: Dr. Phil Oger, Laboratoire de Science de la Terre, CNRS UMR 5570, Universite de Lyon, Ecole Normale Superieure de Lyon, 46 allee d'Italie, F-69364 Lyon Cedex 07, France. poger@ens-lyon.fr
    Keywords: X-ray Spectroscopy ; Glucose Metabolism ; Glucose
    ISSN: 0077-8923
    Source: Cengage Learning, Inc.
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 9
    Language: English
    In: The Journal of biological chemistry, 20 May 2005, Vol.280(20), pp.20069-75
    Description: Pokeweed antiviral protein (PAP) is a ribosome-inactivating protein isolated from the pokeweed plant (Phytolacca americana) that inhibits the proliferation of several plant and animal viruses. We have shown previously that PAP and nontoxic mutants of PAP can directly depurinate brome mosaic virus (BMV) RNA in vitro, resulting in reduced viral protein translation. Here we expand on these initial studies and, using a barley protoplast system, demonstrate that recombinant PAP and nontoxic mutants isolated from E. coli are able to reduce the accumulation of BMV RNAs in vivo. Pretreatment of only BMV RNA3 with PAP prior to transfection of barley protoplasts reduced the accumulation of all BMV RNAs, with a more severe effect on subgenomic RNA4 levels. Using in vitro RNA synthesis assays, we show that a depurinated template causes the BMV replicase to stall at the template nucleotide adjacent to the missing base. These results provide new insight into the antiviral mechanism of PAP, namely that PAP depurination of BMV RNA impedes both RNA replication and subgenomic RNA transcription. These novel activities are distinct from the PAP-induced reduction of viral RNA translation and represent new targets for the inhibition of viral infection.
    Keywords: Bromovirus -- Drug Effects ; N-Glycosyl Hydrolases -- Pharmacology ; Plant Proteins -- Pharmacology ; Virus Replication -- Drug Effects
    ISSN: 0021-9258
    E-ISSN: 1083351X
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 10
    Language: English
    In: International Journal of Pharmaceutics, 30 January 2014, Vol.461(1-2), pp.89-96
    Description: Formulations incorporating different cosmetic grade TiO nanoparticles were developed according to a self-produced protocol on the basis of typical sunscreen cream oil-in-water emulsions. Role of nanoparticles and, more specifically, the impact of two different lipophilic surface treatments on microstructure and stability of the formulations were assessed. Aging of formulations was performed under classical conditions at room temperature or under accelerated conditions at 50 °C, with or without TiO , and was characterized by several tools such as rheology, microscopy, and particle size measurements. Changes in emulsion stability and aggregation state of nanoparticles were followed over time. Destabilization phenomena were identified: under accelerated aging, the formulation without nanoparticles underwent a coalescence occurrence whereas the formulation incorporating nanoparticles was subjected to aggregation of the colloidal particles. Besides, TiO nanoparticles strongly affected the particle–droplets interactions and thus modified the emulsion microstructure with a coating-dependent effect: destabilization mechanisms occur more rapidly with alumina (and) triethoxycaprylylsilane (coating 1) TiO nanoparticles as compared to alumina (and) isopropyl titanium triisostearate (and) triethoxycaprylylsilane crosspolymer (coating 2) nanoparticles.
    Keywords: Titanium Dioxide Nanoparticle ; Sunscreen ; Particle Size Measurement ; Rheology ; Destabilization ; Coating ; Pharmacy, Therapeutics, & Pharmacology
    ISSN: 0378-5173
    E-ISSN: 1873-3476
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. Further information can be found on the KOBV privacy pages