Kooperativer Bibliotheksverbund

Berlin Brandenburg


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  • 1
    Language: English
    In: Archives of otolaryngology--head & neck surgery, March 2006, Vol.132(3), pp.317-26
    Description: To determine expression of cell cycle and apoptotic genes, biochemical analysis of CCL23 and antisense cyclin D1-transfected CCL23 (CCL23AS) cells in the presence of cisplatin was performed. In addition, biochemical analysis of CAL27 cells before and after treatment with cisplatin was performed to determine expression of cell cycle genes. CCL23, CCL23AS, and CAL27 cell lines were treated with cisplatin. Western blot analysis, fluorescence-activated cell sorting, and apoptosis assays were performed. In vitro study of head and neck cancer cell lines CCL23, CCL23AS, and CAL27. CCL23, CCL23AS, and CAL27 cells were treated with cisplatin. Expression of p16, p21, p53, Bcl-xL, Bcl-xS, p27, DP1, MDM2, Bcl-2, c-Jun, and Jun-D were assessed using Western blot analysis. There was increased expression of p16, p21, p53, BCLxL, and BCLxS genes with cisplatin treatment in the CCL23 and CCL23AS cells. Expression of p27, DP1, MDM2, BCL2, c-iun, and jun-D remained unaltered after treatment. There was decreased phosphorylation of Rb protein with complete absence of hyperphosphorylated Rb in the maximally sensitized antisense cyclin D1-transfected (CCL23AS) cells. Fluorescence-activated cell sorter analysis revealed a decreased G2 phase of the cell cycle and an increased proportion of apoptotic cells in the CCL23AS cell line compared with parental CCL23 cells. Cell killing also occurred in the presence of caspase-3 inhibitor. While CCL23 cells contain wild-type p53, the CAL27 cells have a point mutation in codon 193 (A--〉T transversion) of exon 6. However, CAL27 cells still exhibited increased expression of p21 after treatment with cisplatin. These results, in combination with increased expression of the p53 downstream effecter p21, indicate that the cisplatin-induced cell cycle arrest operates through the p16/p53-dependent pathway, and a caspase-independent pathway may be involved. Combination treatment of head and neck squamous cell carcinoma via cell cycle inhibition and cisplatin holds promise as a potential therapy in the clinical setting.
    Keywords: Antineoplastic Agents -- Pharmacology ; Carcinoma, Squamous Cell -- Pathology ; Cisplatin -- Pharmacology ; Cyclin-Dependent Kinase Inhibitor P16 -- Analysis ; Head and Neck Neoplasms -- Pathology ; Tumor Suppressor Protein P53 -- Analysis
    ISSN: 0886-4470
    E-ISSN: 1538361X
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  • 2
  • 3
    Language: English
    In: Cancer Research, 07/15/2016, Vol.76(14 Supplement), pp.3259-3259
    Description: Angiopoeitin-2 (Ang2) is released from endothelial cells only in response to stimulus (e.g. wound healing, tumor growth) and facilitates blood vessel sprouting and inhibits pericyte-endothelial cell interaction via Tie2 signaling. Combination of an anti-Ang2 antibody and aflibercept,...
    Keywords: Cell Survival ; Vascular Endothelial Growth Factor ; Ovarian Cancer ; Retina ; Immunotherapy ; Animal Models ; Angiogenesis ; Pericytes ; Wound Healing ; Tumors ; Clinical Trials ; Metastases ; Endothelial Cells ; Antibodies ; Blood Vessels ; Immunoglobulin G ; Cell Interactions ; Xenografts ; Products;
    ISSN: 0008-5472
    E-ISSN: 1538-7445
    Source: CrossRef
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  • 4
    Language: English
    In: Clinical cancer research : an official journal of the American Association for Cancer Research, 01 October 2005, Vol.11(19 Pt 1), pp.6994-7002
    Description: The purpose of this study was to determine whether curcumin would trigger cell death in the head and neck squamous cell carcinoma (HNSCC) cell lines CCL 23, CAL 27, and UM-SCC1 in a dose-dependent fashion. HNSCC cells were treated with curcumin and assayed for in vitro growth suppression using 3-(4,5-dimethylthiozol-2-yl)-2,5-diphenyl tetrazolium bromide and fluorescence-activated cell sorting analyses. Expression of p16, cyclin D1, phospho-Ikappabeta, and nuclear factor-kappabeta (NF-kappabeta) were measured by Western blotting, gel shift, and immunofluorescence. Addition of curcumin resulted in a dose-dependent growth inhibition of all three cell lines. Curcumin treatment resulted in reduced nuclear expression of NF-kappabeta. This effect on NF-kappabeta was further reflected in the decreased expression of phospho-Ikappabeta-alpha. Whereas the expression of cyclin D1, an NF-kappabeta-activated protein, was also reduced, there was no difference in the expression of p16 at the initial times after curcumin treatment. In vivo growth studies were done using nude mice xenograft tumors. Curcumin was applied as a noninvasive topical paste to the tumors and inhibition of tumor growth was observed in xenografts from the CAL27 cell line. Curcumin treatment resulted in suppression of HNSCC growth both in vitro and in vivo. Our data support further investigation into the potential use for curcumin as an adjuvant or chemopreventive agent in head and neck cancer.
    Keywords: Antineoplastic Agents -- Pharmacology ; Carcinoma, Squamous Cell -- Drug Therapy ; Curcumin -- Pharmacology ; Head and Neck Neoplasms -- Drug Therapy
    ISSN: 1078-0432
    E-ISSN: 15573265
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  • 5
    Language: English
    In: Otolaryngology–Head and Neck Surgery, August 2004, Vol.131(2), pp.P179-P179
    Description: Problem: To determine whether curcumin would trigger cell death in head and neck squamous cell carcinoma (HNSCC) cell lines CCL 23, CAL 27, UMSCC-1, and UMSCC-14 in a dose-dependent fashion. Methods: In-vitro studies included MTT assay and Annexin V-FITC flow cytometry of the HNSCC cells at 8 and 15 hours after treatment with curcumin. In vivo studies were done on HNSCC xenograft tumors grown in nude mice, using curcumin paste applied topically to the tumors daily for 4 weeks. Results: CCL23 cell lines had dose-dependent reduction in cell viability following treatment with curcumin, with nearly 100% cell survival at 100 μM and less than 5% survival at 300 μM. CAL27 cell lines showed reduction of cell viability beginning at 60 μM, and 0% survival at 300 μM. UMSCC-1 cell lines showed reduction in cell viability from 70% at 100 μM to 0% at 200 μM, at both 8 and 15 hours after treatment. UMSCC-14A cell lines showed similar sensitivity, with an average 40% reduction in viability beginning at 100 μM and 0% survival at 200 μM. Flow cytometry demonstrated early and late apoptosis with the curcumin-treated CCL23 and CAL27 cells. The xenograft tumors exhibited more than 50% decrease in size with the curcumin/saline paste. Conclusion: Curcumin has a direct effect on CCL23, CAL27, UMSCC-1, and UMSCC-14, reducing viability in both cell lines in a dose-dependent fashion. In addition, curcumin/saline paste had a significant suppressive effect on HNSCC xenograft tumors, confirming curcumin’s anti-tumor properties in vivo. Significance: To determine whether curcumin would trigger cell death in head and neck squamous cell carcinoma cell lines. Support: None reported.
    Keywords: Medicine
    ISSN: 0194-5998
    E-ISSN: 1097-6817
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  • 6
    In: Antimicrobial Agents and Chemotherapy, 2006, Vol. 50(7), p.2428
    Description: The molecular mechanisms of drug resistance in 19 strains of Vibrio fluvialis isolated from 1998 to 2002 in Kolkata, India, were investigated. Class 1 integrons were detected in eight strains, and four strains were found to carry SXT integrases. In the presence of carbonyl cyanide m-chlorophenylhydrazone or reserpine, all nalidixic acid- and ciprofloxacin-resistant strains became sensitive, suggesting that drug efflux plays a major role in quinolone resistance in V. fluvialis. It was further seen that strains which had MICs of 〉 25 microg/ml for nalidixic acid had a sense mutation (Ser to Ile) at position 83 of the quinolone resistance-determining region of gyrA. All except one of the integron- and SXT integrase-bearing strains belonged to the same ribotype.
    Keywords: Medicine ; Biology ; Pharmacy, Therapeutics, & Pharmacology;
    ISSN: 0066-4804
    ISSN: 00664804
    E-ISSN: 10986596
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  • 7
    Language: English
    In: The Pediatric Infectious Disease Journal, 1983, Vol.2(6), pp.436-441
    ISSN: 0891-3668
    Source: Wolters Kluwer - Ovid - Lippincott Williams & Wilkins (via CrossRef)
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  • 8
    In: Pediatric Research, 1978, Vol.12(S4), p.427
    Description: Of 40 infants born to mothers with positive RPR tests three were symptomatic in the newborn period. After lumbar puncture (LP), these infants were treated with 50,000 μ/kgm of procaine penicillin daily for 10 days. One infant died of syphilitic cirrhosis at 6 months of age; a second had psychomotor retardation at 15 months and one was normal. The remaining 37 infants were asymptomatic at birth. 23 with negative RPR or titers less than their mothers were untreated and did not have LP's. 14 with titers equal to or greater than their mothers had LP's and received one injection of 50,000 units/kgm benzathine penicillin. All were followed at three month intervals until titers were negative. Negative tests were observed in the asymptomatic infants at 6 months in 81 percent and at 12 months in 92 percent. Length of gestation (p 0.05) and birth weight (p 0.05) were lower and cord IgM (p 0.01) were greater in symptomatic infants. Spinal fluid VDRL was negative in all infants. Spinal fluid chemical values in symptomatic and asymptomatic infants were similar. Symptoms at birth was the best predictor of outcome. Currently recommended therapy could not be relied upon to prevent disease.
    Keywords: Medicine;
    ISSN: 0031-3998
    E-ISSN: 15300447
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  • 9
    Language: English
    In: Pediatric infectious disease, 1983, Vol.2(6), pp.436-41
    Description: Seventy-eight newborn infants born to mothers with serologic evidence of syphilis (positive serum rapid plasma reagin and fluorescent treponemal antibody-absorption tests) were prospectively evaluated to derive diagnostic and therapeutic criteria for congenital syphilis. Sixty-one infants were asymptomatic with normal serum IgM and normal roentgenograms (Group I). Eight infants had clinical and/or laboratory evidence of infection at birth (Group II). Nine infants presented with late onset infection (Group III). Elevated serum IgM and abnormal roentgenologic findings were consistently present in symptomatic infants in Groups II and III. Cerebrospinal fluid (CSF) examination was normal in all asymptomatic infants and in all infants with late onset disease. One of the eight infants in Group II examined at birth had positive CSF Venereal Disease Research Laboratory determinations, but all other CSF findings were within normal limits, and a second infant with a slight increase in CSF protein had no clinical evidence of central nervous system (CNS) involvement. Of those asymptomatic infants who returned for follow-up 75% and 100% were seronegative by 3 and 6 months, respectively. The symptomatic infants remained seropositive up to 18 months of age. Infants who had no clinical evidence of CNS involvement at birth remained normal at follow-up and had normal CSF findings. The two infants with CNS symptoms at birth continued to have developmental delay despite normal CSF findings. The incidence of CNS involvement in congenital syphilis appears to be extremely low. The value of routine spinal fluid examination is discussed.
    Keywords: Syphilis, Congenital -- Diagnosis
    ISSN: 0277-9730
    Source: MEDLINE/PubMed (U.S. National Library of Medicine)
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