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Berlin Brandenburg

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  • 1
    Article
    Article
    Language: English
    In: IEEE Spectrum, May 2015, Vol.52(5), pp.27-27
    Description: The first time Ashok Jhunjhunwala and his team helped install an ATM in rural South India, around 2005, the villagers didn't like what they saw: They knew that the new bank notes it issued would be suspected as counterfeit, in contrast to soiled notes, considered authentic because they'd been in circulation.
    Keywords: Engineering ; Physics
    ISSN: 0018-9235
    E-ISSN: 1939-9340
    Source: IEEE Conference Publications
    Source: IEEE Xplore
    Source: IEEE Journals & Magazines 
    Source: IEEE Journals & Magazines
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  • 2
    Language: English
    In: Tetrahedron, 2011, Vol.67(42), pp.8146-8154
    Description: A combinatorial library of 4-(2-hydroxyaryl)-3-nitro-4 -chromenes was synthesized in high yield by C4-SMe substitution in -alkyl/phenyl 4-(methylthio)-3-nitro-4 -chromen-2-amines with a variety of phenols. The reaction always provided C2 substitution in the phenol ring, dictated by hydrogen bond interactions between the phenolic hydroxyl group and the nitro group in 3-nitro-4 -chromenes. Reduction of the nitro group with concomitant hydrolysis of the enamine in 4-(2-hydroxyaryl)-3-nitro-4 -chromenes with Zn, Ac O in AcOH furnished hybrid amino-acid lactone incorporating -tyrosine and phenyl alanine moieties.
    Keywords: Nitroketene ; Electrophlic Substitution ; 4-Aryl-4 H-Chromenes ; Hybrid Amino Acid ; Chemistry
    ISSN: 0040-4020
    E-ISSN: 1464-5416
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  • 3
    In: PLoS ONE, 2013, Vol.8(9)
    Description: The role of mesenchymal stem cells (MSCs) on breast cancer progression, growth and tumorigenesis remains controversial or unknown. In the present study, we investigated the role of MSCs on breast tumor induction and growth in a clinically relevant somatic breast cancer model. We first conducted in vitro studies and found that conditioned media (CM) of RCAS-Neu and RCAS-PyMT breast cancer cell lines and tumor cells themselves dramatically increased the proliferation and motility of MSCs and induced morphological changes of MSCs and differentiation into fibroblast-like cells. In contrast, the CM of MSCs inhibited the proliferation of two breast cancer cell lines by arresting the cell cycle at the G0/G1 phase. In vivo studies revealed that fluorescence dye-labeled MSCs migrated into tumor tissues. Unexpectedly, single or multiple intravenous injections of MSCs did not affect the latency of breast cancer in TVA- transgenic mice induced by intraductal injection of the RCAS vector encoding polyoma middle-T antigen (PyMT) or Neu oncogenes. Moreover, MSCs had no effect on RCAS-Neu tumor growth in a syngeneic ectopic breast cancer model. While our studies consistently demonstrated the ability of breast cancer cells to profoundly induce MSCs migration, differentiation, and proliferation, the anti-proliferative effect of MSCs on breast tumor cells observed in vitro could not be translated into an antitumor activity in vivo , probably reflecting the antagonizing or complex effects of MSCs on tumor environment and tumor cells themselves.
    Keywords: Research Article
    E-ISSN: 1932-6203
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  • 4
    Language: English
    In: The Journal of biological chemistry, 02 October 2015, Vol.290(40), pp.24604-13
    Description: The ATP-binding cassette transporter ABCG1 has an essential role in cellular cholesterol homeostasis, and dysregulation has been associated with a number of high burden diseases. Previous studies reported that ABCG1 is ubiquitinated and degraded via the ubiquitin proteasome system. However, so far the molecular mechanism, including the identity of any of the rate-limiting ubiquitination enzymes, or E3 ligases, is unknown. Using liquid chromatography mass spectrometry, we identified two HECT domain E3 ligases associated with ABCG1, named HUWE1 (HECT, UBA, and WWE domain containing 1, E3 ubiquitin protein ligase) and NEDD4-1 (Neural precursor cell-expressed developmentally down regulated gene 4), of which the latter is the founding member of the NEDD4 family of ubiquitin ligases. Silencing both HUWE1 and NEDD4-1 in cells overexpressing human ABCG1 significantly increased levels of the ABCG1 monomeric and dimeric protein forms, however ABCA1 protein expression was unaffected. In addition, ligase silencing increased ABCG1-mediated cholesterol export to HDL in cells overexpressing the transporter as well as in THP-1 macrophages. Reciprocally, overexpression of both ligases resulted in a significant reduction in protein levels of both the ABCG1 monomeric and dimeric forms. Like ABCG1, ABCG4 protein levels and cholesterol export activity were significantly increased after silencing both HUWE1 and NEDD4-1 in cells overexpressing this closely related ABC half-transporter. In summary, we have identified for the first time two E3 ligases that are fundamental enzymes in the post-translational regulation of ABCG1 and ABCG4 protein levels and cellular cholesterol export activity.
    Keywords: ABC Transporter ; E3 Ubiquitin Ligase ; Cholesterol Regulation ; Membrane Lipid ; Post-Transcriptional Regulation ; ATP-Binding Cassette Transporters -- Metabolism ; Endosomal Sorting Complexes Required for Transport -- Metabolism ; Lipids -- Chemistry ; Ubiquitin-Protein Ligases -- Metabolism
    E-ISSN: 1083-351X
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  • 5
    Language: English
    In: The American Journal of Physiology, Dec, 2012, Vol.303(6), p.G1356(9)
    Description: Acetaldehyde is accumulated at high concentrations in the colonic lumen following ethanol administration. Previous studies demonstrated that acetaldehyde disrupts intestinal epithelial tight junctions and increases paracellular permeability. In the present study, we investigated the role of PP2A in the acetaldehyde-induced disruption of intestinal epithelial tight junctions. Caco-2 cell monolayers were exposed to 200-600 [micro]M acetaldehyde for varying times, and the epithelial barrier function was evaluated by measuring transepithelial electrical resistance and inulin permeability. Acetaldehyde treatment resulted in a time-dependent increase in inulin permeability and redistribution of occludin and ZO-1 from the intercellular junctions. Treatment of cells with fostriecin (a PP2A-selective inhibitor) or knockdown of PP2A by siRNA blocked acetaldehyde-induced increase in inulin permeability and redistribution of occludin and ZO-1. The effects of fostriecin and acetaldehyde were confirmed in mouse intestine ex vivo. Acetaldehyde-induced tight junction disruption and barrier dysfunction were also attenuated by a PP2A-specific inhibitory peptide, TPDYFL. Coimmunoprecipitation studies showed that acetaldehyde increased the interaction of PP2A with occludin and induced dephosphorylation of occludin on threonine residues. Fostriecin and TPDYFL significantly reduced acetaldehyde-induced threonine dephosphorylation of occludin. Acetaldehyde failed to change the level of the methylated form of PP2A-C suhunit. However, genistein (a tyrosine kinase inhibitor) blocked acetaldehyde-induced association of PP2A with occludin and threonine dephosphorylation of occludin. These results demonstrate that acetaldehyde-induced disruption of tight junctions is mediated by PP2A translocation to tight junctions and dephosphorylation of occludin on threonine residues. intestinal epithelium; dephosphorylation; barrier function doi: 10.1152/ajpgi.00526.2011
    Keywords: Isoflavones ; Phosphatases ; Permeability ; Monomolecular Films ; Acetaldehyde
    ISSN: 0002-9513
    Source: Cengage Learning, Inc.
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  • 6
    Language: English
    In: Tetrahedron Letters, 2009, Vol.50(27), pp.3836-3839
    Description: Base-catalyzed reaction of the nitroketene , -acetals and the ring substituted 2-hydroxybenzaldehydes afforded a combinatorial library of the 2-alkylamino-3-nitro-4-alkylsulfanyl 4 -chromenes in excellent yields. Nucleophilic displacement of the C4 alkylsulfanyl group with different thiols afforded 4 -chromenes with structural diversity.
    Keywords: Nmsm ; 2-Hydroxybenzaldehydes ; Michael Addition ; 4 H-Chromenes ; 2:1 Adducts ; Chemistry
    ISSN: 0040-4039
    E-ISSN: 1873-3581
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  • 7
    In: Journal of Cytology, 2012, Vol.29(2), p.133-134
    Description: Two patients aged 50 years and 49 years, presented with worsening renal function at 4 months and 10½ months respectively, following renal transplant. Both cases were on triple immunosuppressants including tacrolimus, azathioprine and steroids.
    Keywords: Cells ; Cellular Biology ; Urine ; Infections ; Transplants & Implants ; Kidneys;
    ISSN: 0970-9371
    E-ISSN: 09745165
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  • 8
    Language: English
    In: BBA - Molecular and Cell Biology of Lipids, July 2015, Vol.1851(7), pp.956-964
    Description: The ATP-binding cassette (ABC) transporter, ABCG1, is a lipid exporter involved in removal of cholesterol from cells that has been investigated for its role in foam cells formation and atherosclerosis. The mechanism by which ABC lipid transporters bind and recognise their substrates is currently unknown. In this study, we identify a critical region in the final transmembrane domain of ABCG1, which is essential for its export function and stabilisation by cholesterol, a post-translational regulatory mechanism that we have recently identified as dependent on protein ubiquitination. This transmembrane region contains several Cholesterol Recognition/interaction Amino acid Consensus (CRAC) motifs, and its inverse CARC motifs. Mutational analyses identify one CRAC motif in particular with Y667 at its core, that is especially important for transport activity to HDL as well as stability of the protein in the presence of cholesterol. In addition, we present a model of how cholesterol docks to this CRAC motif in an energetically favourable manner. This study identifies for the first time how ABCG1 can interact with cholesterol via a functional CRAC domain, which provides the first insight into the substrate–transporter interaction of an ABC lipid exporter.
    Keywords: Abcg1 ; Cholesterol Transport ; Crac Motifs ; Cholesterol Binding Domains ; Biology ; Chemistry ; Anatomy & Physiology
    ISSN: 1388-1981
    E-ISSN: 1879-2618
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  • 9
    Language: English
    In: Wash. U. J.L. & Pol'y, Vol.48 pp.1-347, 2015
    Keywords: Entrepreneurship; Groundbreaking; Practitioners; Collaboration; Developments; Participants; Restorative; Alternative; Negotiation; Scholarship; Civil Procedure; Governments; International Law; International Trade Law; Legal Ethics
    Source: Academic Law Reviews (LexisNexis®)
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  • 10
    Language: English
    In: BMC Cancer, 01 May 2011, Vol.11(1), p.194
    Description: Abstract Background Major histocompatibility complex class I-related chain A and B (MICA/B) are two stress-inducible ligands that bind the immunoreceptor NKG2D and play an important role in mediating the cyotoxicity of NK and T cells. In this study, we sought to study MICA/B expression in pancreatic cancer and to determine whether and how genotoxic drugs such as gemcitabine can affect MICA/B expression and natural killer cytotoxity. Methods Seven pancreatic cancer cell lines were analyzed for MICA/B expression by flow cytometry and for their sensitivity to NK-92 cell killing by a 51Cr release assay. MICA/B expression in tumor tissues and sera of pancreatic cancer was analyzed by immunohistochemical staining (IHC) and ELISA, respectively. Results Two MICA/B-positive cell lines were sensitive to the cytotoxic activity of NK-92 cells. Other two MICA/B-positive cell lines and three MICA/B-negative cell lines were resistant to NK-92 cell killing. MICA/B expression was positive in 17 of 25 (68%) pancreatic ductal adenocarcinomas but not in normal pancreatic ductal epithelial cells. Serum MICA/B levels were significantly elevated in patients with pancreatic adenocarcinomas but did not correlate with the stage of pancreatic cancer and patient survival. Gemcitabine therapy led to increased serum MICA levels in 6 of 10 patients with detectable serum MICA. Allopurinol, an inhibitor of xanthine oxidoreductase that converts xanthine to uric acid, blocked uric acid production, MICA/B expression, and sensitivity to NK-92 cell killing toward a PANC-1 cancer cell line exposed to radiation and two genotoxic drugs, gemcitabine and 5-fluorouracil. Conclusions The levels of MICA/B expression in serum and tissue of pancreatic cancer are elevated. DNA damage-induced MICA/B expression is mediated through increased uric acid production.
    Keywords: Pancreatic Cancer ; Mica/B ; Gemcitabine ; Uric Acid ; Allopurinol ; DNA Damage ; Medicine
    ISSN: 1471-2407
    E-ISSN: 1471-2407
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