UID:
edoccha_9961427245802883
Format:
1 streaming video file (47 min., 58 sec.) :
,
sound, color.
,
004758
Series Statement:
Henry Stewart talks
Note:
Retrieved April 13, 2024, from https://hstalks.com/bs/444/.
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Introduction -- Repeat proteins -- Why undertake protein design -- Repeat protein vs. globular protein -- Tetratricopeptide repeat (TPR) -- Genomic distribution of proteins with tandem TPRs -- Consensus design of a TPR motif -- CTPR1, CTPR2 and CTPR3 -- Introducing co-variation, enhancing stability -- Describing TPR stability by a simple model -- Lots of proteins are needed for a model -- Stability as a function of number of tandem TPRs -- Predictions of the Ising model -- Protein stability on a residue-specific basis -- Protein stability - a range of stability -- Other behaviors explained by the Ising model -- What do the long TPRs look like? -- Crystal structure of CTPR8 -- Use of crystal structure of CTPR8 -- Super-position of TPR domain of OGT and CTPR8 -- Functional design: ligand binding -- Functional design: specific contacts -- The nature of ligand-binding sites in proteins -- CTPR - conserved and active residues -- Hypervariability defines the ligand-binding site -- Analogous to antibody hypervariable regions -- Hypervariablity and binding site - general result? -- Hypervariablity and binding site is a general result -- ANK repeats -- Ligand binding to ANK repeats - binding specificitiy -- Functional design -- Electrostatic interactions modulate binding affinity -- Binding is specific -- Split GFP detection of protein-protein intercations -- Split GFP reports on affinity and specificity -- Identifying TPR domains with novel specificities -- Summary.
Language:
English
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