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  • 1
    Research Dataset
    Research Dataset
    DOE Geothermal Data Repository; Flint Geothermal, LLC
    Language: English
    Description: Modeled Bouger-Corrected Gravity data was extracted from the Pan American Center for Earth and Environmental Studies Gravity Database of the U.S. at http://irpsrvgis08.utep.edu/viewers/Flex/GravityMagnetic/GravityMagnetic_CyberShare/ on 2/29/2012. The downloaded text file was opened in an Excel spreadsheet. This spreadsheet data was then converted into an ESRI point shapefile in UTM Zone 13 NAD27 projection, showing location and gravity (in milligals). This data was then converted to grid and then contoured using ESRI Spatial Analyst. Data from From University of Texas: Pan American Center for Earth and Environmental Studies...
    Keywords: Geothermal Energy ; Geothermal ; Gravity ; Bouger ; Colorado ; Shapefile ; Point Shapefile ; Line Shapefile ; Shape File ; Gis ; Arcgis ; Geospatial ; Contour ; West ; Central ; West-Central
    Source: DataCite
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  • 2
    Language: English
    In: PLoS ONE, 01 January 2014, Vol.9(9), p.e108758
    Description: Aurora kinase inhibitors displayed activity in pre-clinical neuroblastoma models. Here, we studied the effects of the pan-aurora kinase inhibitor tozasertib (VX680, MK-0457) and the aurora kinase inhibitor alisertib (MLN8237) that shows some specificity for aurora kinase A over aurora kinase B in a panel of neuroblastoma cell lines with acquired drug resistance. Both compounds displayed anti-neuroblastoma activity in the nanomolar range. The anti-neuroblastoma mechanism included inhibition of aurora kinase signalling as indicated by decreased phosphorylation of the aurora kinase substrate histone H3, cell cycle inhibition in G2/M phase, and induction of apoptosis. The activity of alisertib but not of tozasertib was affected by ABCB1 expression. Aurora kinase inhibitors induced a p53 response and their activity was enhanced in combination with the MDM2 inhibitor and p53 activator nutlin-3 in p53 wild-type cells. In conclusion, aurora kinases are potential drug targets in therapy-refractory neuroblastoma, in particular for the vast majority of p53 wild-type cases.
    Keywords: Sciences (General)
    E-ISSN: 1932-6203
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  • 3
    Language: English
    In: Forensic Science International, 10 October 2012, Vol.222(1-3), pp.335-339
    Description: In Europe, the blowfly genus is represented in Forensic Entomology mainly by the species , , and . In the US, is rarely recorded as a carrion breeding species but usually as a more or less exclusive parasite of frogs and toads. We present three forensic cases from different European countries reporting, for the first time, on human bodies that were found close to lakes, wetlands, or riversides. To use this species for post-mortem interval estimations, thermal development data is needed. The first step is accurate identification by morphological and molecular means. Therefore, we analysed a 611 bp part of the mitochondrial COI region for 23 specimens of from 9 different geographical regions, all of which give the same haplotype. Differences within the haplotype varied by up to 0.2%. Comparison between the haplotype found and those published on GenBank showed up to 1.2% variance. Moreover, we present an updated key for the morphological identification of the third larval instars of European spp. of forensic importance, adding not only , but also which was recorded in Europe for the first time about 20 years ago.
    Keywords: Forensic Entomology ; Blowflies ; Lucilia Silvarum ; Case Report ; Cytochrome C Oxidase I ; Identification Key ; Public Health
    ISSN: 0379-0738
    E-ISSN: 1872-6283
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  • 4
    Language: English
    In: PLoS ONE, Sept 30, 2014, Vol.9(9)
    Description: Aurora kinase inhibitors displayed activity in pre-clinical neuroblastoma models. Here, we studied the effects of the pan-aurora kinase inhibitor tozasertib (VX680, MK-0457) and the aurora kinase inhibitor alisertib (MLN8237) that shows some specificity for aurora kinase A over aurora kinase B in a panel of neuroblastoma cell lines with acquired drug resistance. Both compounds displayed anti-neuroblastoma activity in the nanomolar range. The anti-neuroblastoma mechanism included inhibition of aurora kinase signalling as indicated by decreased phosphorylation of the aurora kinase substrate histone H3, cell cycle inhibition in G2/M phase, and induction of apoptosis. The activity of alisertib but not of tozasertib was affected by ABCB1 expression. Aurora kinase inhibitors induced a p53 response and their activity was enhanced in combination with the MDM2 inhibitor and p53 activator nutlin-3 in p53 wild-type cells. In conclusion, aurora kinases are potential drug targets in therapy-refractory neuroblastoma, in particular for the vast majority of p53 wild-type cases.
    Keywords: Tumor Proteins ; Apoptosis ; Phosphotransferases ; Neuroblastoma
    ISSN: 1932-6203
    Source: Cengage Learning, Inc.
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  • 5
    In: PLoS ONE, 2018, Vol.13(12)
    Description: The bluebottle blow fly Calliphora vicina is a common species distributed throughout Europe that can play an important role as forensic evidence in crime investigations. Developmental rates of C . vicina from distinct populations from Germany and England were compared under different temperature regimes to explore the use of growth data from different geographical regions for local case work. Wing morphometrics and molecular analysis between these populations were also studied as indicators for biological differences. One colony each of German and English C . vicina were cultured at the Institute of Legal Medicine in Frankfurt, Germany. Three different temperature regimes were applied, two constant (16°C & 25°C) and one variable (17–26°C, room temperature = RT). At seven time points (600, 850, 1200, 1450, 1800, 2050, and 2400 accumulated degree hours), larval lengths were measured; additionally, the durations of the post feeding stage and intrapuparial metamorphosis were recorded. For the morphometric and molecular study, 184 females and 133 males from each C . vicina population (Germany n = 3, England n = 4) were sampled. Right wings were measured based on 19 landmarks and analyzed using canonical variates analysis and discriminant function analysis. DNA was isolated from three legs per specimen (n = 61) using 5% chelex. A 784 bp long fragment of the mitochondrial cytochrome b gene was sequenced; sequences were aligned and phylogenetically analyzed. Similar larval growth rates of C . vicina were found from different geographic populations at different temperatures during the major part of development. Nevertheless, because minor differences were found a wider range of temperatures and sampling more time points should be analyzed to obtain more information relevant for forensic case work. Wing shape variation showed a difference between the German and English populations ( P 〈0.0001). However, separation between the seven German and English populations at the smaller geographic scale remained ambiguous. Molecular phylogenetic analysis by maximum likelihood method could not unambiguously separate the different geographic populations at a national (Germany vs England) or local level.
    Keywords: Research Article ; Research And Analysis Methods ; People And Places ; People And Places ; People And Places ; Biology And Life Sciences ; Medicine And Health Sciences ; Biology And Life Sciences ; Biology And Life Sciences ; Biology And Life Sciences ; Computer And Information Sciences ; Medicine And Health Sciences ; Social Sciences ; Research And Analysis Methods
    E-ISSN: 1932-6203
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  • 6
    Language: English
    In: Parasitology Research, 2012, Vol.110(6), pp.2325-2332
    Description: Deoxyribonucleic acid (DNA)-based insect identification has become a routine and accurate tool in forensic entomology. In the present study, we demonstrate the utility of the mitochondrial DNA cytochrome oxidase I gene “barcoding region” as a universal marker for molecular identification of forensically important Diptera. We analyzed 111 specimens belonging to 13 species originating from Frankfurt am Main, Germany (Calliphoridae: Calliphora vicina , Calliphora vomitoria , Lucilia ampullacea , Lucilia caesar , Lucilia illustris , Lucilia sericata , Lucilia silvarum , Phormia regina , Protophormia terraenovae ; Piophilidae: Parapiophila vulgaris ; Muscidae: Hydrotaea dentipes , Hydrotaea ignava , Hydrotaea similis ). Intraspecific variation ranged from 0 to 1.17% and interspecific variation occurred between 1.17% and 15.21%. Although differences within species were generally less than among species, divergence percentages overlapped due to low interspecific nucleotide divergence of the recently separated sister species L. caesar and L. illustris . However, all species formed distinct monophyletic clades and thus the cytochrome oxidase 1 (COI) barcode has been shown suitable for clear differentiation and identification of forensically relevant Diptera in Germany.
    Keywords: Biomedicine ; Medical Microbiology ; Immunology ; Microbiology ; Zoology;
    ISSN: 0932-0113
    E-ISSN: 1432-1955
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  • 7
    Language: English
    In: International Journal of Legal Medicine, 2013, Vol.127(1), pp.243-249
    Description: Necrophagous blow fly larvae can provide accurate estimates of the minimum postmortem interval in death investigations. During larval development, predictable morphological changes occur and measurements of weight, length, and width are compared to species-specific growth curves for reliable age estimates. However, aging blow fly pupae is more challenging because morphological and anatomical changes are not visible with the naked eye. Thus, delicate preparation of the pupae or rearing to the adult stage seems unavoidable. Conversely, metamorphosis evokes a remodelling of the larval shape to adult structures, and gene expression analysis potentially serves as a molecular tool to mirror the ageing process of a pupa. The present study focuses on the differential expression of two newly described, arbitrarily named genes ( 15_2 , 2014192 ) and two previously identified genes ( actin , arylphorin receptor ) during Calliphora vicina (Diptera: Calliphoridae) metamorphosis. Quantification through real-time PCR revealed significant up- and downregulation of these transcripts found to be temperature dependent and age specific, hence, a new possibility to age forensically important blow fly pupae.
    Keywords: Forensic entomology ; Postmortem interval ; Calliphora vicina ; Differentially expressed genes ; Metamorphosis
    ISSN: 0937-9827
    E-ISSN: 1437-1596
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  • 8
    Language: English
    In: International Journal of Legal Medicine, 2014, Vol.128(3), pp.565-573
    Description: The estimation of the minimum postmortem interval can be implemented by age estimation of corpse-associated primary colonizers such as the blow flies (Diptera: Calliphoridae). In cases where pupae represent the oldest stages found on a body, their age must be added to the duration of prepupal development to estimate the minimum postmortem interval. Although methods to age blow fly larvae have been well established using morphology, length or weight and age estimation of pupae has proved challenging. In a previous work, we quantified the changes in mRNA levels of four differentially expressed genes during the metamorphosis of Calliphora vicina pupae, hence representing molecular markers for pupal age (i.e., time elapsed since pupariation). Here, we demonstrate how these data can be used to estimate pupal age with inverse prediction. We present three blind studies conducted under various conditions and show that age of C . vicina pupae can be well estimated based on gene expression data. As these data are quantitative and can be processed automatically, gene expression has the potential to outperform morphological analysis in age estimation of forensically relevant blow fly pupae.
    Keywords: Forensic entomology ; Calliphora vicina ; Quantitative real-time PCR ; Gene expression ; Blow fly pupae
    ISSN: 0937-9827
    E-ISSN: 1437-1596
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  • 9
    Language: English
    In: International Journal of Legal Medicine, 2014, Vol.128(6), pp.1001-1011
    Description: Estimating the post mortem interval (PMI min ) by age determination of blow fly larvae has been well-established for moderate temperatures. Low-temperature developmental data is only available sparsely and usually does not take overwintering strategies into account. The blow fly Calliphora vicina hibernates by diapausing in the third larval stage extending the duration of this developmental stage up to several weeks or even months. As the diagnosis of the diapause status is not possible by morphological characteristics, PMI min estimations might be biased during the cold season if only based on age determination of third instar larvae of C . vicina . Molecular markers were searched for which allows one to identify diapause in larvae. Expression analysis of 19 genes was performed in diapausing and non-diapausing larvae. Three genes encoding for heat shock proteins ( hsp23 , hsp24 and hsp70 ) were found to be up-regulated distinctly in diapausing larvae and at 1 day in non-diapausing larvae. If several larvae are subjected to an analysis, a high variance in the expression level of the gene encoding for the anterior fat body protein is a further marker for diapause. The present study proves the potential use of gene expression analysis as a suitable diagnosis tool for diapause in C . vicina .
    Keywords: Calliphora vicina ; Forensic entomology ; Minimum post mortem interval ; Age estimation ; Heat shock proteins ; Diapause
    ISSN: 0937-9827
    E-ISSN: 1437-1596
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  • 10
    Research Dataset
    Research Dataset
    DOE Geothermal Data Repository; Flint Geothermal, LLC
    Language: English
    Description: These line shapefiles trace apparent topographic and air-photo lineaments in various counties in Colorado. It was made in order to identify possible fault and fracture systems that might be conduits for geothermal fluids, as part of a DOE reconnaissance geothermal exploration program. Geothermal fluids commonly utilize fault and fractures in competent rocks as conduits for fluid flow. Geothermal exploration involves finding areas of high near-surface temperature gradients, along with a suitable "plumbing system" that can provide the necessary permeability. Geothermal power plants can sometimes be built where temperature and flow rates are high. This line shapefile is an attempt to use desktop GIS to delineate possible faults and fracture orientations and locations in highly prospective areas prior to an initial site visit. Geochemical sampling and geologic mapping could then be centered around these possible faults and fractures. To do this, georeferenced topographic maps and aerial photographs were utilized in an existing GIS, using ESRI ArcMap 10.0 software. The USA_Topo_Maps and World_Imagery map layers were chosen from the GIS Server at server.arcgisonline.com, using a UTM Zone 13 NAD27 projection. This line shapefile was then constructed over that which appeared to be through-going structural lineaments in both the aerial photographs and topographic...
    Keywords: Geothermal Energy ; Geothermal ; Structure ; Lineaments ; Air-Photos ; Colorado ; Alamosa County ; Archeluta County ; Chaffee County ; Delta County ; Dolores County ; Eagle County ; Garfield County ; Mineral County ; Park County ; Routt County ; San Miguel County ; Shapefile ; Shape File ; Exploration ; Gis ; Arcgis ; Geospatial
    Source: DataCite
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