Format:
Online-Ressource (XX, 632 p. 75 illus., 47 illus. in color, online resource)
ISBN:
9780387698052
Series Statement:
SpringerLink
Content:
Thomas Ried
Content:
Genetically-engineered mouse models for cancer research have become invaluable tools for studying cancer biology and evaluating novel therapeutic approaches. This volume focuses on state-of-the-art methods for generating, analyzing and validating such models for studying aspects of human cancer biology. Additionally, these models are emerging as important pre-clinical systems in which to test cancer prevention and therapeutic strategies in order to select compounds for testing in clinical trials
Note:
Description based upon print version of record
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Genetically Engineered Mice for Cancer Research; Foreword; Preface; Contents; Contributors; Chapter 1: Overview of Designing Genetically Engineered Mouse (GEM) Models; 1.1 Introduction; 1.2 Has the GEM Already Been Made?; 1.3 Considerations in Choosing ES Cell Genetic Background; 1.4 Considerations for Simple Gene Knockout Vector Design; 1.5 Knockin-SNPs; 1.6 Conditional Gene Modifications; 1.7 Reversible Gene Targeting; 1.8 Isogenic DNA; 1.9 Length of Homologous Arms; 1.10 Distance Between Homologous Arms; 1.11 Heterologous Elements Within Homologous Arms; 1.12 Positive Selection
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1.13 Negative Selection1.14 Pretesting Diagnostic Procedures; 1.15 Recombineering Systems; 1.16 BAC Transgenics Versus Subtle Mutations; 1.17 Summary; 1.18 Brief Historical Perspective; 1.18.1 Development of GEM Field; References; Chapter 2: The Use of Cre-loxP Technology and Inducible Systems to Generate Mouse Models of Cancer; 2.1 Introduction; 2.2 Cre-LoxP System; 2.3 Cre-LoxP-Mediated Gene Inactivation; 2.3.1 Generation of a Conditional Mutant Allele in Mice; 2.3.2 Deletion of the Neo Gene from a Conditional Mutant Allele; 2.3.3 Tissue-Specific Conditional Knockout Mice
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2.3.3.1 Cre-loxP-Mediated Knockout of SMAD4 in Multiple Tissues2.3.3.2 Cre-loxP-Mediated Knockout of BRCA1 in Breast Cancer Research; 2.4 Cre-loxP-Mediated Gene Activation; 2.4.1 Activation of Oncogenes Using the Cre-loxP Technology; 2.4.2 Activation of Tumor Suppressor Genes Using the Cre-LoxP Technology; 2.5 Conclusion and Future Directions; References; Chapter 3: Using Recombineering Technology to Create Genetically Engineered Mouse Models; 3.1 Introduction; 3.2 In Vivo Cloning in E. coli; 3.3 Recombineering Using Bacteriophage Recombination Genes
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3.4 Recombineering Using Defective l -Prophage3.5 Generating Knockout Mouse Models; 3.5.1 Subcloning or Retrieving Genomic DNA from BAC; 3.5.2 Inserting Selectable Markers; 3.6 Conditional Gene Modifications; 3.7 High-Throughput Recombineering; 3.8 Multiple Alterations; 3.9 Generating Transgenic Constructs; 3.10 Recombineering-Based Methods for Generating Subtle Alterations; 3.10.1 A Selection-Counter Selection Method to Generate Subtle Alterations in BACs; 3.10.2 Modifications Using Single-Stranded Oligonucleotides; 3.11 Humanized Mouse Models of Human Diseases
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3.12 Inserting Nonselectable Cassettes3.12.1 Generating Cre/Flp Lines for Conditional Knockout Mouse Models; 3.12.2 Generating Reporter Lines; 3.13 Concluding Remarks; References; Chapter 4: Insertional Mutagenesis for Generating Mouse Models of Cancer; 4.1 Introduction; 4.2 Viral Models of Cancer Induced by Insertional Mutagenesis; 4.3 Transposon-Based Models of Cancer Induced by Insertional Mutagenesis; 4.4 Important Considerations in Creating and Interpreting Results from Mouse Models of Cancer Based on Insertional Mutagenesis; 4.5 Conclusions and Future Progress; References
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Chapter 5: The RCAS/TVA Somatic Gene Transfer Method in Modeling Human Cancer
Additional Edition:
ISBN 9780387698038
Additional Edition:
Druckausg. ISBN 978-038-769-803-8
Language:
English
DOI:
10.1007/978-0-387-69805-2
URL:
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