UID:
almahu_9949251332102882
Format:
1 online resource (XI, 324 p. 45 illus., 17 illus. in color.)
Edition:
1st ed. 2016.
ISBN:
1-4939-3299-3
Series Statement:
Methods in Molecular Biology, 1388
Content:
This volume provides detailed descriptions of prevailing and novel techniques used by experts in the study of PTEN function in disease and biology. The book begins with chapters exploring methods to detect expression levels of PTEN in normal and diseased human specimens; methods to evaluate specific PTEN function in brain cancer; methods that utilize a new biosensor to measure PTEN regulation; and techniques to measure post-transcriptional regulation of PTEN by micoRNAs and ceRNAs. Other chapters present methods describing novel techniques to detect PTEN localization and previously unstudied structural features of PTEN measured through X-Ray Crystallography and Hydrogen Deuterium Exchange Mass Spectrometry. The book concludes with methods to study PTEN function in model organisms including mice and C. elegans. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, PTEN: Methods and Protocols is a valuable collection of methodologies and protocols useful to researchers who are interested in the PTEN field.
Note:
Bibliographic Level Mode of Issuance: Monograph
,
PTEN: history of a tumor suppressor / Leonardo Salmena -- PTEN at 18 : still growing / Olena Gorbenko and Vuk Stambolic -- Immunopathologic assessment of PTEN expression / Mireia Castillo-Martin, Tin Htwe Thin, Ana Collazo Lorduy, and Carlos Cordon-Cardo -- Measurement of PTEN by flow cytometry / Jolm F. Woolley and Leonardo Salmena -- Detecting PTEN and PI3K signaling in brain / Guo Zhu and Suzanne J. Baker -- Germline PTEN mutation analysis for PTEN hamartoma tumor syndrome / Joanne Ngeow and Charis Eng -- Methods for assessing the in vivo role of PTEN in glucose homeostasis / Cynthia T. Luk, Stephanie A. Schroer, and Minna Woo -- Rapid detection of dynamic PTEN regulation in living cells using intramolecular BRET / Stanislas Misticone, Evelyne Lima-Fernandes, and Mark G.H. Scott -- Methods for the identification of PTEN-targeting MicroRNAs / Andrea Tuccoli, Marianna Vitiello, Andrea Marranci, Francesco Russo, and Laura Poliseno -- Posttranscriptional regulation of PTEN by competing endogenous RNAs / Yvonne Tay and Pier Paolo Pandolfi -- In cell and in vitro assays to measure PTEN ubiquitination / Amit Gupta, Helene Maccario, Nisha Kriplani, and Nicholas R. Leslie -- Assessing PTEN subcellular localization / Anabel Gil, José I. López, and Rafael Pulido -- Methods to study PTEN in mitochondria and endoplasmic reticulum / Sonia Missiroli, Claudia Morganti, Carlotta Giorgi, and Paolo Pinton -- Methods in the study of PTEN structure : X-ray crystallography and hydrogen deuterium exchange mass spectrometry / Glenn R. Masson, John E. Burke, and Roger L. Williams -- Methods for PTEN in stem cells and cancer stem cells / Suzanne Schubbert, Jing Jiao, Marcus Ruscetti, Jonathan Nakashima, Shumin Wu, Hong Lei, Qinzhi Xu, Wenkai Yi, Haichuan Zhu, and Hong Wu -- Modeling cancer-associated mutations of PTEN in mice / Antonella Papa and Pier Paolo Pandolfi -- C. elegans methods to study PTEN / Shanqing Zheng and Ian D. Chin-Sang.
,
English
Additional Edition:
ISBN 1-4939-3297-7
Language:
English
Keywords:
Laboratory Manuals
;
Laboratory Manuals
DOI:
10.1007/978-1-4939-3299-3
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