Format:
XI, 342 S.
,
Ill., graph. Darst.
,
26 cm
ISBN:
9781493923915
Series Statement:
Methods in molecular biology 1276
Content:
Includes bibliographical references
Note:
Mapping the Escherichia coli transcription elongation complex with exonuclease III
,
Purification of bacterial RNA polymerase : tools and protocols
,
Monitoring translocation of multisubunit RNA polymerase along the DNA with fluorescent base analogues
,
In vitro and in vivo methodologies for studying the sigma 54-dependent transcription
,
Methods for the assembly and analysis of in vitro transcription-coupled-to-translation systems
,
Site-specific incorporation of probes into RNA polymerase by unnatural-amino-acid mutagenesis and staudinger-bertozzi ligation
,
Reconstitution of factor-dependent, promoter proximal pausing in Drosophila nuclear extracts
,
Direct competition assay for transcription fidelity
,
Single-stranded DNA aptamers for functional probing of bacterial RNA polymerase
,
Biochemical analysis of transcription termination by RNA polymerase III from yeast Saccharomyces cerevisiae
,
Use of RNA polymerase molecular beacon assay to measure RNA polymerase interactions with model promoter fragments
,
Preparation of cDNA libraries for high-throughput RNA sequencing analysis of RNA 5' ends
,
In situ footprinting of E. coli transcription elongation complex with chloroacetaldehyde
,
Using solutes and kinetics to probe large conformational changes in the steps of transcription initiation
,
Manipulating archaeal systems to permit analyses of transcription elongation-termination decisions in vitro
,
Purification of active RNA polymerase I from yeast
,
Transcription in archaea : preparation of Methanocaldococcus jannaschii transcription machinery
,
Transcription in archaea : in vitro transcription assays for mjRNAP
,
Experimental analysis of hFACT action during Pol II transcription in vitro
,
ChIP-Seq for genome-scale analysis of bacterial DNA- binding proteins
Additional Edition:
9781493923922
Additional Edition:
Online-Ausg. Artsimovitch, Irina Bacterial Transcriptional Control New York, NY : Humana Press, 2015 9781493923922
Language:
English
Subjects:
Biology
Keywords:
Genregulation
;
Laboratory Manuals
;
Aufsatzsammlung
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