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Berlin Brandenburg

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  • 1
    In: Transplantation, 1995, Vol.59(7), pp.1023-1028
    Description: Expression of adhesion molecules and human leukocyte antigens on the surface of hepatocytes (HC) may play an important role in the immune reaction in different types of infectious and noninfectious hepatitis, liver graft rejection, and autoimmune liver diseases.The aim of this study was to evaluate the influence of the proinflammatory cytokines IFN-α, IFN-γ, and IL-1α on the expression of intercellular adhesion molecule- 1 (ICAM-1) and HLA-A, -B, -C, and -DR on highly purified primary human HC in cell culture. Expression was assessed by semiquantitative measurement of HC in cell culture by means of computer-aided fluorometry after immunofluorescent labeling. Avidinbiotin- immunoperoxidase staining was applied on parallel cultures to evaluate cell purity (〉99%) and to confirm the results obtained by fluorometry.ICAM-1 was expressed constitutively on untreated HC in vitro. Stimulation of HC with IFN-γ and IL-1α for 24 hr resulted in an increase of ICAM-1 expression. Cultured HC were moderately HLA-A, -B, and -C positive, but HLA-DR negative. Stimulation of HC with 500 U/ml IFN-g for 72 hr resulted in an increase of HLA-A, -B, -C, and -DR expression, whereas stimulation with 10 U/ml IL-1α for 72 hr had no influence. By using 5000 U/ml IFN-α for 72 hr, we achieved an increase of HLA-A, -B, and -C expression; effects on the other tested antigens were not significant. In contrast to endothelial cells and transformed human hepatocytic cell lines, ICAM-1 on HC was changed more intensively by IFN-γ than by IL-1α. Furthermore, the results reveal differences in HLA and ICAM-1 expression between HC in vivo and in vitro.
    Keywords: Inflammation ; Hepatocytes ; Histocompatibility Antigen HLA ; Man ; Cellular Components ; Cytokines ; Dr Determinant ; A Determinant ; B Determinant ; C Determinant ; Intercellular Adhesion Molecule 1 ; A Determinant ; B Determinant ; C Determinant ; Dr Determinant ; Cytokines ; Intercellular Adhesion Molecule 1;
    ISSN: 0041-1337
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  • 2
    In: Transplantation, 2000, Vol.69(4), pp.588-597
    Description: BACKGROUND.: Cyclosporine A (CsA) and tacrolimus prevent proliferation but not transendothelial migration of alloreactive lymphocytes into donor organs. As a result, serious adverse effects, such as nephrotoxicity and neurotoxicity, have been observed under CsA/tacrolimus therapy. The incorporation of new drugs with infiltration blocking properties might enhance the efficacy of the current immunosuppressive protocol, allowing lower CsA/tacrolimus dosage. Because Ca plays a critical role in cell-cell interaction, the Ca-channel blocker verapamil might be a good cany.didate for supporting CsA/tacrolimus-based therap METHODS.: A T-cell endothelial cell coculture model or immobilized immunoglobulin G globulin chimeras were employed to investigate how S- and R- verapamil interfere with the lymphocytic infiltration process. The expression and arrangement of membranous adhesion receptors and cytoskeletal F-actin filaments were analyzed by fluorometric method in the presence of. verapamil. RESULTS.: Both verapamil enantiomers strongly inhibited lymphocyte infiltration. CD4 and CD8 T-cells were influenced to a similar extent with regard to horizontal locomotion (CD4=CD8), but to a different extent with regard to adhesion and penetration (CD4 〉 CD8). Moreover, penetration was blocked to a higher extent than was adhesion. ID50-values were 31 μM (CD4-adhesion) and 11 μM (CD4-penetration). Verapamil reduced P-selectin expression on endothelial cells and effectively down-regulated binding of T-cells to immobilized P-selectin immunoglobulin G globulins (ID50=4.4 μM; CD4). A verapamil-induced reduction of intracellular F-actin in T-lymphocytes was proven to be mainly responsible for diminished cell locomotion. CONCLUSIONS.: The prevention of CD4 T-cell penetration by verapamil might argue for its use as an adjunct to CsA/tacrolimus-based immunosuppressive therapy.
    Keywords: Immunosuppression ; Endothelium ; Lymphocytes T ; Immunosuppressive Agents ; Cell Motility ; Verapamil ; Calcium Channel Blockers ; Experimental ; Function ; Immunology ; Calcium Channel Blockers ; Cell Motility ; Immunology ; Verapamil;
    ISSN: 0041-1337
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  • 3
    Language: English
    In: Cancer Research, 07/15/2016, Vol.76(14 Supplement), pp.1532-1532
    ISSN: 0008-5472
    E-ISSN: 1538-7445
    Source: CrossRef
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  • 4
    In: PLoS ONE, 2014, Vol.9(12)
    Description: The increasing incidence of implant-associated infections induced by Staphylococcus aureus (SA) in combination with growing resistance to conventional antibiotics requires novel therapeutic strategies. In the current study we present the first application of the biofilm-penetrating antimicrobial peptide lysostaphin in the context of bone infections. In a standardized implant-associated bone infection model in mice beta-irradiated lysostaphin-coated titanium plates were compared with uncoated plates. Coating of the implant was established with a poly(D,L)-lactide matrix (PDLLA) comprising lysostaphin formulated in a stabilizing and protecting solution (SPS). All mice were osteotomized and infected with a defined count of SA. Fractures were fixed with lysostaphin-coated locking plates. Plates uncoated or PDLLA-coated served as controls. All mice underwent debridement and lavage on Days 7, 14, 28 to determine the bacterial load and local immune reaction. Fracture healing was quantified by conventional radiography. On Day 7 bacterial growth in the lavages of mice with lysostaphin-coated plates showed a significantly lower count to the control groups. Moreover, in the lysostaphin-coated plate groups complete fracture healing were observed on Day 28. The fracture consolidation was accompanied by a diminished local immune reaction. However, control groups developed an osteitis with lysis or destruction of the bone and an evident local immune response. The presented approach of terminally sterilized lysostaphin-coated implants appears to be a promising therapeutic approach for low grade infection or as prophylactic strategy in high risk fracture care e.g. after severe open fractures.
    Keywords: Research Article ; Medicine And Health Sciences ; Physical Sciences ; Research And Analysis Methods
    E-ISSN: 1932-6203
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  • 5
    Language: English
    In: Journal of Applied Physics, May 15, 2011, Vol.109(10), p.103102-1-103102-6
    Description: Highly efficient small molecule organic light emitting diodes and organic solar cells is demonstrated based on the p-i-n-type structure by using the fluorinated fullerene molecule [C.sub.60][F.sub.36] as p-dopant in the hole transport layer. The low volatility and high thermal stability properties have made [C.sub.60][F.sub.36] very attractive for the usage as p-dopant in a broad spectrum of organic p-i-n devices like organic light emitting diodes, solar cells, memories or transistors.
    Keywords: Carbon Compounds -- Thermal Properties ; Fullerenes -- Thermal Properties ; Leds -- Analysis
    ISSN: 0021-8979
    Source: Cengage Learning, Inc.
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  • 6
    Language: English
    In: The Journal of biological chemistry, 09 November 2018, Vol.293(45), pp.17559-17573
    Description: The supramolecular organization of membrane proteins (MPs) is sensitive to environmental changes in photosynthetic organisms. Isolation of MP supercomplexes from the green algae , which are believed to contribute to cyclic electron flow (CEF) between the cytochrome complex (Cyt- ) and photosystem...
    Keywords: Chlamydomonas ; Cytochrome B6f Complex ; Electron Transfer Complex ; Membrane Protein ; Photosynthesis ; Protein Cross-Linking ; Chlamydomonas Reinhardtii -- Enzymology ; Cytochrome B6f Complex -- Metabolism ; Photosystem I Protein Complex -- Metabolism
    E-ISSN: 1083-351X
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  • 7
    Language: English
    In: Plant physiology, April 2015, Vol.167(4), pp.1566-78
    Description: In Chlamydomonas reinhardtii, the LIGHT-HARVESTING COMPLEX STRESS-RELATED PROTEIN3 (LHCSR3) protein is crucial for efficient energy-dependent thermal dissipation of excess absorbed light energy and functionally associates with photosystem II-light-harvesting complex II (PSII-LHCII) supercomplexes. Currently, it is unknown how LHCSR3 binds to the PSII-LHCII supercomplex. In this study, we investigated the role of PHOTOSYSTEM II SUBUNIT R (PSBR) an intrinsic membrane-spanning PSII subunit, in the binding of LHCSR3 to PSII-LHCII supercomplexes. Down-regulation of PSBR expression diminished the efficiency of oxygen evolution and the extent of nonphotochemical quenching and had an impact on the stability of the oxygen-evolving complex as well as on PSII-LHCII-LHCSR3 supercomplex formation. Its down-regulation destabilized the PSII-LHCII supercomplex and strongly reduced the binding of LHCSR3 to PSII-LHCII supercomplexes, as revealed by quantitative proteomics. PHOTOSYSTEM II SUBUNIT P deletion, on the contrary, destabilized PHOTOSYSTEM II SUBUNIT Q binding but did not affect PSBR and LHCSR3 association with PSII-LHCII. In summary, these data provide clear evidence that PSBR is required for the stable binding of LHCSR3 to PSII-LHCII supercomplexes and is essential for efficient energy-dependent quenching and the integrity of the PSII-LHCII-LHCSR3 supercomplex under continuous high light.
    Keywords: Proteomics ; Chlamydomonas Reinhardtii -- Genetics ; Light-Harvesting Protein Complexes -- Metabolism ; Oxygen -- Metabolism ; Photosystem II Protein Complex -- Metabolism
    ISSN: 00320889
    E-ISSN: 1532-2548
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  • 8
    Language: English
    In: Earth and Planetary Science Letters, May 15, 2012, Vol.331-332, p.187(14)
    Description: To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.epsl.2012.03.019 Byline: Cyril Giry, Thomas Felis, Martin Kolling, Denis Scholz, Wei Wei, Gerrit Lohmann, Sander Scheffers Keywords: coral Sr/Ca; southern Caribbean climate; sea surface temperature; seasonality; interannual to multidecadal variability; ENSO teleconnection Abstract: Proxy reconstructions of tropical Atlantic sea surface temperature (SST) that extend beyond the period of instrumental observations have primarily focused on centennial to millennial variability rather than on seasonal to multidecadal variability. Here we present monthly-resolved records of Sr/Ca (a proxy of SST) from fossil annually-banded Diploria strigosa corals from Bonaire (southern Caribbean Sea). The individual corals provide time-windows of up to 68years length, and the total number of 295years of record allows for assessing the natural range of seasonal to multidecadal SST variability in the western tropical Atlantic during snapshots of the mid- to late Holocene. Comparable to modern climate, the coral Sr/Ca records reveal that mid- to late Holocene SST was characterised by clear seasonal cycles, persistent quasi-biennial and prominent interannual as well as inter- to multidecadal-scale variability. However, the magnitude of SST variations on these timescales has varied over the last 6.2ka. The coral records show increased seasonality during the mid-Holocene consistent with climate model simulations indicating that southern Caribbean SST seasonality is induced by insolation changes on orbital timescales, whereas internal dynamics of the climate system play an important role on shorter timescales. Interannual SST variability is linked to ocean-atmosphere interactions of Atlantic and Pacific origin. Pronounced interannual variability in the western tropical Atlantic is indicated by a 2.35ka coral, possibly related to a strengthening of the variability of the El Nino/Southern Oscillation throughout the Holocene. Prominent inter- to multidecadal SST variability is evident in the coral records and slightly more pronounced in the mid-Holocene. We finally argue that our coral data provide a target for studying Holocene climate variability on seasonal and interannual to multidecadal timescales, when using further numerical models and high-resolution proxy data. Article History: Received 13 May 2011; Revised 20 December 2011; Accepted 13 March 2012 Article Note: (miscellaneous) Editor: P. DeMenocal
    Keywords: El Nino -- Analysis ; Southern Oscillation -- Analysis ; Corals -- Analysis ; Holocene Paleogeography -- Analysis
    ISSN: 0012-821X
    Source: Cengage Learning, Inc.
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  • 9
    In: Journal of the American Ceramic Society, September 1971, Vol.54(9), pp.474-474
    ISSN: 0002-7820
    E-ISSN: 1551-2916
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  • 10
    Language: English
    In: Critical Care, Jan 13, 2011, Vol.15, p.R20
    Description: Introduction Deregulated apoptosis and overshooting neutrophil functions contribute to immune and organ dysfunction in sepsis and multiple organ failure (MOF). In the present study, we determined the role of soluble Fas (sFas) in the regulation of posttraumatic neutrophil extrinsic apoptosis and the development of sepsis. Methods Forty-seven major trauma patients, 18 with and 29 without sepsis development during the first 10 days after trauma, were enrolled in this prospective study. Seventeen healthy volunteers served as controls. Blood samples from severely injured patients were analyzed at day 1, day 5 and day 9 after major trauma. sFas levels, plasma levels of neutrophil elastase (PMNE) and levels of interleukin (IL)-6 were quantified by enzyme-linked immunosorbent assay and related to patients' Sequential Organ Failure Assessment (SOFA) score and Multiple Organ Dysfunction Score (MODS). Neutrophil apoptosis was determined by propidium iodide staining of fragmented DNA and flow cytometry. sFas-mediated effects on neutrophil apoptosis were investigated in cells cultured with agonistic anti-Fas antibodies in the presence of recombinant sFas, sFas-depleted serum or untreated serum from septic patients. Results Serum levels of sFas in patients who later developed sepsis were significantly increased at day 5 (P [less than] 0.01) and day 9 (P [less than] 0.05) after trauma compared with patients with uneventful recovery. Apoptosis of patient neutrophils was significantly decreased during the observation period compared with control cells. Moreover, Fas-mediated apoptosis of control neutrophils was efficiently inhibited by recombinant sFas and serum from septic patients. Depletion of sFas from septic patient sera diminished the antiapoptotic effects. In septic patients, sFas levels were positively correlated with SOFA at day 1 (r = 0.7, P [less than] 0.001), day 5 (r = 0.62, P [less than] 0.01) and day 9 (r = 0.58, P [less than] 0.01) and with PMNE and leukocyte counts (r = 0.49, P [less than] 0.05 for both) as well as MODS at day 5 (r = 0.56, P [less than] 0.01) after trauma. Conclusions Increased sFas in patients with sepsis development impairs neutrophil extrinsic apoptosis and shows a positive correlation with the organ dysfunction scores and PMNE. Therefore, sFas might be a therapeutic target to prevent posttrauma hyperinflammation and sepsis.
    Keywords: Apoptosis -- Physiological Aspects ; Apoptosis -- Genetic Aspects ; Apoptosis -- Research ; Injuries -- Complications And Side Effects ; Injuries -- Patient Outcomes ; Injuries -- Research ; Multiple Organ Failure -- Risk Factors ; Multiple Organ Failure -- Development And Progression ; Multiple Organ Failure -- Genetic Aspects ; Multiple Organ Failure -- Research ; Sepsis -- Risk Factors ; Sepsis -- Development And Progression ; Sepsis -- Genetic Aspects ; Sepsis -- Research
    ISSN: 1364-8535
    Source: Cengage Learning, Inc.
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