Format:
1 Online-Ressource (XVIII, 149 Seiten, 35565 KB)
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Illustrationen, Diagramme
Content:
The incorporation of proteins in artificial materials such as membranes offers great opportunities to avail oneself the miscellaneous qualities of proteins and enzymes perfected by nature over millions of years. One possibility to leverage proteins is the modification with artificial polymers. To obtain such protein-polymer conjugates, either a polymer can be grown from the protein surface (grafting-from) or a pre-synthesized polymer attached to the protein (grafting-to). Both techniques were used to synthesize conjugates of different proteins with thermo-responsive polymers in this thesis. First, conjugates were analyzed by protein NMR spectroscopy. Typical characterization techniques for conjugates can verify the successful conjugation and give hints on the secondary structure of the protein. However, the 3-dimensional structure, being highly important for the protein function, cannot be probed by standard techniques. NMR spectroscopy is a unique method allowing to follow even small alterations in the protein structure. A mutant ...
Note:
Dissertation Universität Potsdam 2022
Additional Edition:
Erscheint auch als Druck-Ausgabe Mathieu-Gaedke, Maria Grafting-to and grafting-from proteins Potsdam, 2021
Language:
English
Keywords:
Hochschulschrift
DOI:
10.25932/publishup-54292
URN:
urn:nbn:de:kobv:517-opus4-542921
URL:
https://doi.org/10.25932/publishup-54292
URL:
https://nbn-resolving.org/urn:nbn:de:kobv:517-opus4-542921
URL:
https://d-nb.info/1255276509/34
Author information:
Böker, Alexander 1973-
Author information:
Schwaneberg, Ulrich 1969-
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