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  • 1
    Online Resource
    Online Resource
    Chichester :Wiley-Blackwell,
    UID:
    almafu_9959328495102883
    Format: 1 online resource (xvi, 333 pages, 8 pages of plates)
    ISBN: 9780470711675 , 0470711671 , 9780470711576 , 0470711574 , 9780470711620 , 0470711620
    Content: Genomics research has made significant advances in recent years. In this book, a team of internationally-renowned researchers share the most up-to-date information in a field that has in recent years switched emphasis from gene identification to functional genomics and the characterization of genes and gene products. This volume approaches this complex subject with a broad perspective to supply the reader with a vital overview of genomics and its derivative fields, with a focus on pivotal issues such as data analysis. Expansive and current, this book is a comprehensive research guide that desc.
    Note: Front Matter -- Colour Plates -- High-Resolution Analysis of Genomic Copy Number Changes / Mario Hermsen, Jordy Coffa, Bauke Ylstra, Gerrit Meijer, Hans Morreau, Ronald van Eijk, Jan Oosting, Tom van Wezel -- Identification of Polymorphic Markers for Genetic Mapping / Daniel C Koboldt, Raymond D Miller -- Genotyping and LOH Analysis on Archival Tissue using SNP Arrays / Ronald van Eijk, Anneke Middeldorp, Esther H Lips, Marjo van Puijenbroek, Hans Morreau, Jan Oosting, Tom van Wezel -- Genetic Mapping of Complex Traits / Nancy L Saccone -- RNA Amplification Strategies: Toward Single-Cell Sensitivity / Natalie Stickle, Norman N Iscove, Carl Virtanen, Mary Barbara, Carolyn Modi, Toni Di Berardino, Ellen Greenblatt, Ted Brown, Neil Winegarden -- Real-Time Quantitative RT-PCR for mRNA Profiling / Stephen A Bustin, Tania Nolan -- Gene Expression in Mammalian Cells / F̌lix Recillas-Targa, Georgina Guerrero, Mart̕n Escamilla-del-Arenal, Ȟctor Rinc̤n-Arano -- Using Yeast Two-Hybrid Methods to Investigate Large Numbers of Binary Protein Interactions / Panagoula Charalabous, Jonathan Woodsmith, Christopher M Sanderson -- Prediction of Protein Function / Hon Nian Chua -- Elucidating Gene Function through use of Genetically Engineered Mice / Mary P Heyer, C̀tia Feliciano, Jôo Peca, Guoping Feng -- Delivery Systems for Gene Transfer / Charlotte Lawson, Louise Collins -- Gene Therapy Strategies: Constructing an AAV Trojan Horse / M Ian Phillips, Edilamar M de Oliveira, Leping Shen, Yao Liang Tang, Keping Qian -- An Introduction to Proteomics Technologies for the Genomics Scientist / David B Friedman -- Index.
    Additional Edition: Print version: Genomics. Hoboken : Wiley, 2011 ISBN 9780470711576
    Language: English
    Keywords: Electronic books. ; Electronic books. ; Electronic books.
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Online Resource
    Online Resource
    Hoboken :Wiley-Blackwell,
    UID:
    almahu_9948314681702882
    Format: xvi, 333 p., [8] leaves of plates : , ill.
    Edition: Electronic reproduction. Ann Arbor, MI : ProQuest, 2015. Available via World Wide Web. Access may be limited to ProQuest affiliated libraries.
    Language: English
    Keywords: Electronic books.
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  • 3
    Online Resource
    Online Resource
    Hoboken :Wiley-Blackwell,
    UID:
    edoccha_9958129493402883
    Format: 1 online resource (360 p.)
    ISBN: 1-282-88357-7 , 9786612883576 , 0-470-71167-1 , 0-470-71162-0
    Series Statement: Essential Methods
    Content: Genomics research has made significant advances in recent years. In this book, a team of internationally-renowned researchers share the most up-to-date information in a field that has in recent years switched emphasis from gene identification to functional genomics and the characterization of genes and gene products. This volume approaches this complex subject with a broad perspective to supply the reader with a vital overview of genomics and its derivative fields, with a focus on pivotal issues such as data analysis. Expansive and current, this book is a comprehensive research guide that desc
    Note: Description based upon print version of record. , Genomics; Contents; List of Contributors; Preface; 1 High-Resolution Analysis of Genomic Copy Number Changes; 2 Identification of Polymorphic Markers for Genetic Mapping; 3 Genotyping and LOH Analysis on Archival Tissue Using SNP Arrays; 4 Genetic Mapping of Complex Traits; 5 RNA Amplification Strategies: Toward Single-Cell Sensitivity; 6 Real-Time Quantitative RT-PCR for mRNA Profiling; 7 Gene Expression in Mammalian Cells; 8 Using Yeast Two-Hybrid Methods to Investigate Large Numbers of Binary Protein Interactions; 9 Prediction of Protein Function , 10 Elucidating Gene Function through Use of Genetically Engineered Mice11 Delivery Systems for Gene Transfer; 12 Gene Therapy Strategies: Constructing an AAV Trojan Horse; 13 An Introduction to Proteomics Technologies for the Genomics Scientist; Index , English
    Additional Edition: ISBN 0-470-71157-4
    Language: English
    Library Location Call Number Volume/Issue/Year Availability
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  • 4
    Online Resource
    Online Resource
    Totowa, NJ :Humana Press :
    UID:
    edocfu_9959000000002883
    Format: 1 online resource (XV, 425 p. 115 illus., 4 illus. in color.)
    Edition: 2nd ed. 2008.
    ISBN: 1-59745-188-6
    Series Statement: Methods in Molecular Biology, 439
    Content: Since the first edition, published in 2001, genomics research has taken great strides. In Genomics Protocols, 2nd Edition, a team of expert researchers share the most current information in a field that has recently switched emphasis from gene identification to functional genomics and the characterization of genes and gene products. This volume approaches its complex subject with a broad perspective to supply its reader with a vital overview of genomics and its derivative fields, with a focus on pivotal issues such as data analysis. Following the highly successful Methods in Molecular Biology™ series format, the chapters provide easy-to-follow laboratory protocols, lists of the necessary materials, and the excellent Notes section, which offers tips on troubleshooting and avoiding known pitfalls. Expansive and current, Genomics Protocols, 2nd Edition is the perfect update for researchers working in this integral, growing field.
    Note: Bibliographic Level Mode of Issuance: Monograph , Whole Genome Amplification with Phi29 DNA Polymerase to Enable Genetic or Genomic Analysis of Samples of Low DNA Yield -- Scanning for DNA Variants by Denaturant Capillary Electrophoresis -- Identification of SNPs, or Mutations in Sequence Chromatograms -- BeadArray-Based Genotyping -- Microsatellite-Based Candidate Gene Linkage Analysis Studies -- Full Complexity Genomic Hybridization on 60-mer Oligonucleotide Microarrays for Array Comparative Genomic Hybridization (aCGH) -- Detection of Copy Number Changes at Multiple Loci in DNA Prepared from Formalin-Fixed, Paraffin-Embedded Tissue by Multiplex Ligation-Dependent Probe Amplification -- Application of Microarrays for DNA Methylation Profiling -- Genomewide Identification of Protein Binding Locations Using Chromatin Immunoprecipitation Coupled with Microarray -- Transcriptional Profiling of Small Samples in the Central Nervous System -- Quantitative Expression Profiling of RNA from Formalin-Fixed, Paraffin-Embedded Tissues Using Randomly Assembled Bead Arrays -- Expression Profiling of microRNAs in Cancer Cells: Technical Considerations -- Identification of Disease Biomarkers by Profiling of Serum Proteins Using SELDI-TOF Mass Spectrometry -- The Applicability of a Cluster of Differentiation Monoclonal Antibody Microarray to the Diagnosis of Human Disease -- Protein Profiling Based on Two-Dimensional Difference Gel Electrophoresis -- Quantitative Protein Profiling by Mass Spectrometry Using Isotope-Coded Affinity Tags -- Quantitative Protein Profiling by Mass Spectrometry Using Label-Free Proteomics -- Using 2D-LC-MS/MS to Identify Francisella tularensis Peptides in Extracts from an Infected Mouse Macrophage Cell Line -- Baculovirus Expression Vector System: An Emerging Host for High-Throughput Eukaryotic Protein Expression -- Coimmunoprecipitation and Proteomic Analyses -- Tandem Affinity Purification Combined with Mass Spectrometry to Identify Components of Protein Complexes -- Mammalian Two-Hybrid Assay for Detecting Protein-Protein Interactions in Vivo -- Detection of Protein-Protein Interactions in Live Cells and Animals with Split Firefly Luciferase Protein Fragment Complementation -- Subcellular Localization of Intracellular Human Proteins by Construction of Tagged Fusion Proteins and Transient Expression in COS-7 Cells -- GeneFAS: GeneFAS: A Tool for the Prediction of Gene function Using Multiple Sources of Data -- Comparative Genomics-Based Prediction of Protein Function -- Design, Manufacture, and Assay of the Efficacy of siRNAs for Gene Silencing. , English
    Additional Edition: ISBN 1-58829-871-X
    Language: English
    Library Location Call Number Volume/Issue/Year Availability
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  • 5
    Online Resource
    Online Resource
    Totowa, NJ :Humana Press :
    UID:
    edoccha_9959000000002883
    Format: 1 online resource (XV, 425 p. 115 illus., 4 illus. in color.)
    Edition: 2nd ed. 2008.
    ISBN: 1-59745-188-6
    Series Statement: Methods in Molecular Biology, 439
    Content: Since the first edition, published in 2001, genomics research has taken great strides. In Genomics Protocols, 2nd Edition, a team of expert researchers share the most current information in a field that has recently switched emphasis from gene identification to functional genomics and the characterization of genes and gene products. This volume approaches its complex subject with a broad perspective to supply its reader with a vital overview of genomics and its derivative fields, with a focus on pivotal issues such as data analysis. Following the highly successful Methods in Molecular Biology™ series format, the chapters provide easy-to-follow laboratory protocols, lists of the necessary materials, and the excellent Notes section, which offers tips on troubleshooting and avoiding known pitfalls. Expansive and current, Genomics Protocols, 2nd Edition is the perfect update for researchers working in this integral, growing field.
    Note: Bibliographic Level Mode of Issuance: Monograph , Whole Genome Amplification with Phi29 DNA Polymerase to Enable Genetic or Genomic Analysis of Samples of Low DNA Yield -- Scanning for DNA Variants by Denaturant Capillary Electrophoresis -- Identification of SNPs, or Mutations in Sequence Chromatograms -- BeadArray-Based Genotyping -- Microsatellite-Based Candidate Gene Linkage Analysis Studies -- Full Complexity Genomic Hybridization on 60-mer Oligonucleotide Microarrays for Array Comparative Genomic Hybridization (aCGH) -- Detection of Copy Number Changes at Multiple Loci in DNA Prepared from Formalin-Fixed, Paraffin-Embedded Tissue by Multiplex Ligation-Dependent Probe Amplification -- Application of Microarrays for DNA Methylation Profiling -- Genomewide Identification of Protein Binding Locations Using Chromatin Immunoprecipitation Coupled with Microarray -- Transcriptional Profiling of Small Samples in the Central Nervous System -- Quantitative Expression Profiling of RNA from Formalin-Fixed, Paraffin-Embedded Tissues Using Randomly Assembled Bead Arrays -- Expression Profiling of microRNAs in Cancer Cells: Technical Considerations -- Identification of Disease Biomarkers by Profiling of Serum Proteins Using SELDI-TOF Mass Spectrometry -- The Applicability of a Cluster of Differentiation Monoclonal Antibody Microarray to the Diagnosis of Human Disease -- Protein Profiling Based on Two-Dimensional Difference Gel Electrophoresis -- Quantitative Protein Profiling by Mass Spectrometry Using Isotope-Coded Affinity Tags -- Quantitative Protein Profiling by Mass Spectrometry Using Label-Free Proteomics -- Using 2D-LC-MS/MS to Identify Francisella tularensis Peptides in Extracts from an Infected Mouse Macrophage Cell Line -- Baculovirus Expression Vector System: An Emerging Host for High-Throughput Eukaryotic Protein Expression -- Coimmunoprecipitation and Proteomic Analyses -- Tandem Affinity Purification Combined with Mass Spectrometry to Identify Components of Protein Complexes -- Mammalian Two-Hybrid Assay for Detecting Protein-Protein Interactions in Vivo -- Detection of Protein-Protein Interactions in Live Cells and Animals with Split Firefly Luciferase Protein Fragment Complementation -- Subcellular Localization of Intracellular Human Proteins by Construction of Tagged Fusion Proteins and Transient Expression in COS-7 Cells -- GeneFAS: GeneFAS: A Tool for the Prediction of Gene function Using Multiple Sources of Data -- Comparative Genomics-Based Prediction of Protein Function -- Design, Manufacture, and Assay of the Efficacy of siRNAs for Gene Silencing. , English
    Additional Edition: ISBN 1-58829-871-X
    Language: English
    Library Location Call Number Volume/Issue/Year Availability
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  • 6
    Online Resource
    Online Resource
    Totowa, NJ :Humana Press :
    UID:
    almahu_9949251536602882
    Format: 1 online resource (XV, 425 p. 115 illus., 4 illus. in color.)
    Edition: 2nd ed. 2008.
    ISBN: 1-59745-188-6
    Series Statement: Methods in Molecular Biology, 439
    Content: Since the first edition, published in 2001, genomics research has taken great strides. In Genomics Protocols, 2nd Edition, a team of expert researchers share the most current information in a field that has recently switched emphasis from gene identification to functional genomics and the characterization of genes and gene products. This volume approaches its complex subject with a broad perspective to supply its reader with a vital overview of genomics and its derivative fields, with a focus on pivotal issues such as data analysis. Following the highly successful Methods in Molecular Biology™ series format, the chapters provide easy-to-follow laboratory protocols, lists of the necessary materials, and the excellent Notes section, which offers tips on troubleshooting and avoiding known pitfalls. Expansive and current, Genomics Protocols, 2nd Edition is the perfect update for researchers working in this integral, growing field.
    Note: Bibliographic Level Mode of Issuance: Monograph , Whole Genome Amplification with Phi29 DNA Polymerase to Enable Genetic or Genomic Analysis of Samples of Low DNA Yield -- Scanning for DNA Variants by Denaturant Capillary Electrophoresis -- Identification of SNPs, or Mutations in Sequence Chromatograms -- BeadArray-Based Genotyping -- Microsatellite-Based Candidate Gene Linkage Analysis Studies -- Full Complexity Genomic Hybridization on 60-mer Oligonucleotide Microarrays for Array Comparative Genomic Hybridization (aCGH) -- Detection of Copy Number Changes at Multiple Loci in DNA Prepared from Formalin-Fixed, Paraffin-Embedded Tissue by Multiplex Ligation-Dependent Probe Amplification -- Application of Microarrays for DNA Methylation Profiling -- Genomewide Identification of Protein Binding Locations Using Chromatin Immunoprecipitation Coupled with Microarray -- Transcriptional Profiling of Small Samples in the Central Nervous System -- Quantitative Expression Profiling of RNA from Formalin-Fixed, Paraffin-Embedded Tissues Using Randomly Assembled Bead Arrays -- Expression Profiling of microRNAs in Cancer Cells: Technical Considerations -- Identification of Disease Biomarkers by Profiling of Serum Proteins Using SELDI-TOF Mass Spectrometry -- The Applicability of a Cluster of Differentiation Monoclonal Antibody Microarray to the Diagnosis of Human Disease -- Protein Profiling Based on Two-Dimensional Difference Gel Electrophoresis -- Quantitative Protein Profiling by Mass Spectrometry Using Isotope-Coded Affinity Tags -- Quantitative Protein Profiling by Mass Spectrometry Using Label-Free Proteomics -- Using 2D-LC-MS/MS to Identify Francisella tularensis Peptides in Extracts from an Infected Mouse Macrophage Cell Line -- Baculovirus Expression Vector System: An Emerging Host for High-Throughput Eukaryotic Protein Expression -- Coimmunoprecipitation and Proteomic Analyses -- Tandem Affinity Purification Combined with Mass Spectrometry to Identify Components of Protein Complexes -- Mammalian Two-Hybrid Assay for Detecting Protein-Protein Interactions in Vivo -- Detection of Protein-Protein Interactions in Live Cells and Animals with Split Firefly Luciferase Protein Fragment Complementation -- Subcellular Localization of Intracellular Human Proteins by Construction of Tagged Fusion Proteins and Transient Expression in COS-7 Cells -- GeneFAS: GeneFAS: A Tool for the Prediction of Gene function Using Multiple Sources of Data -- Comparative Genomics-Based Prediction of Protein Function -- Design, Manufacture, and Assay of the Efficacy of siRNAs for Gene Silencing. , English
    Additional Edition: ISBN 1-58829-871-X
    Language: English
    Library Location Call Number Volume/Issue/Year Availability
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