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  • 1
    Language: English
    In: Biochemistry, 29 March 2011, Vol.50(12), pp.2048-60
    Description: In response to stress small organic compounds termed osmolytes are ubiquitously accumulated in all cell types to regulate the intracellular solvent quality and to counteract the deleterious effect on the stability and function of cellular proteins. Given the evidence that destabilization of the native state of a protein either by mutation or by environmental changes triggers the aggregation in the neurodegenerative pathologies, the modulation of the intracellular solute composition with osmolytes is an attractive strategy to stabilize an aggregating protein. Here we report the effect of three natural osmolytes on the in vivo and in vitro aggregation landscape of huntingtin exon 1 implicated in the Huntington's disease. Trimethylamine N-oxide (TMAO) and proline redirect amyloid fibrillogenesis of the pathological huntingtin exon 1 to nonamyloidogenic amorphous assemblies via two dissimilar molecular mechanisms. TMAO causes a rapid formation of bulky amorphous aggregates with minimally exposed surface area, whereas proline solubilizes the monomer and suppresses the accumulation of early transient aggregates. Conversely, glycine-betaine enhances fibrillization in a fashion reminiscent of the genesis of functional amyloids. Strikingly, none of the natural osmolytes can completely abrogate the aggregate formation; however, they redirect the amyloidogenesis into alternative, nontoxic aggregate species. Our study reveals new insights into the complex interactions of osmoprotectants with polyQ aggregates.
    Keywords: Exons ; Mutation ; Biological Products -- Pharmacology ; Mutant Proteins -- Chemistry ; Nerve Tissue Proteins -- Chemistry ; Osmosis -- Drug Effects ; Protein Multimerization -- Drug Effects
    ISSN: 00062960
    E-ISSN: 1520-4995
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  • 2
    Language: English
    In: Journal of the American College of Cardiology, 20 September 2011, Vol.58(13), pp.e25-e25
    Description: To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.jacc.2011.01.075 Byline: Andreas Schuster ([cor]), Simon G. Duckett ([cor]), Erik Hedstrom ([cor]), Amedeo Chiribiri ([cor]), Georg Techen (a ), Eike Nagel ([cor]) Author Affiliation: ([cor]) King's College London British Heart Foundation (BHF) Centre of Excellence; National Institute of Health Research (NIHR) Biomedical Research Centre at Guy's and St. Thomas' NHS Foundation Trust; Wellcome Trust and Engineering and Physical Sciences Research Council (EPSRC) Medical Engineering Centre; Division of Imaging Sciences and Biomedical Engineering; The Rayne Institute, St. Thomas' Hospital, London, United Kingdom (a ) Zitha-Clinic, Luxembourg City, Luxembourg Article History: Received 24 January 2011; Accepted 31 January 2011 Article Note: (footnote) Dr. Nagel has received significant grant support from Bayer Schering Pharma and Philips Healthcare.
    Keywords: Medicine
    ISSN: 0735-1097
    E-ISSN: 1558-3597
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  • 3
    Language: English
    In: Journal of Colloid And Interface Science, 01 July 2012, Vol.377(1), pp.251-261
    Description: ► CTAB micelles in PBS buffer characterized by ensemble as well as single molecule fluorescence techniques. ► Results show no distinct effect of probes on the fundamental micellar parameters. ► FRET between probes unravels intra- and intermicellar interactions. The well-known cationic surfactant hexadecyltrimethylammonium bromide (CTAB) was used as a model carrier to study drug–carrier interactions with fluorescence probes (5-hexadecanoylaminofluorescein (HAF) and 2,10-bis-(3-aminopropyloxy)dibenzo[a,j]perylene-8,16-dione (NIR 628) by applying ensemble as well as single molecule fluorescence techniques. The impact of the probes on the micelle parameters (critical micelle concentration, average aggregation number, hydrodynamic radius) was investigated under physiological conditions. In the presence of additional electrolytes, such as buffer, the critical micelle concentration decreased by a factor of about 10. In contrast, no influence of the probes on the critical micelle concentration and on average aggregation number was observed. The results show that HAF does not affect the characteristics of CTAB micelles. Analyzing fluorescence correlation spectroscopy data and time-resolved anisotropy decays in terms of the “two-step” in combination with the “wobbling-in-cone” model, it was proven that HAF and NIR 628 are differently associated with the micelles. Based on ensemble and single molecule fluorescence experiments, intra- and intermicellar energy transfer process between the two dyes were probed and characterized.
    Keywords: Hexadecyltrimethylammonium Bromide ; 5-Hexadecanoylaminofluorescein ; 2,10-Bis-(3-Aminopropyloxy)Dibenzo[a,J]Perylene-8,16-Dione ; Fluorescence Correlation Spectroscopy ; Fluorescence Anisotropy ; Single-Molecule Fret ; Engineering ; Chemistry
    ISSN: 0021-9797
    E-ISSN: 1095-7103
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  • 4
    Language: English
    In: Current Opinion in Biotechnology, Feb, 2014, Vol.25, p.103(8)
    Description: To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.copbio.2013.09.010 Byline: Natascha Techen, Iffat Parveen, Zhiqiang Pan, Ikhlas A Khan Abstract: Author Affiliation: (1) National Center for Natural Products Research and Research Institute of Pharmaceutical Sciences, Department of Pharmacognosy, School of Pharmacy, University of Mississippi, P.O. Box 1848, MS 38677, USA (2) USDA-ARS-NPURU, P.O. Box 8048, University, MS 38677, USA (3) School of Pharmacy, King Saud University, Saudi Arabia
    Keywords: Dna ; Bar Codes ; Herbal Medicine
    ISSN: 0958-1669
    Source: Cengage Learning, Inc.
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  • 5
    Language: English
    In: Helvetica Chimica Acta, November 2013, Vol.96(11), pp.2046-2067
    Description: Fluorescence probes consisting of well‐established fluorophores in combination with rigid molecular rods based on spirane‐type structures were investigated with respect to their fluorescence properties under different solvent conditions. The attachment of the dyes was accomplished by 1,3‐dipolar cycloaddition between alkynes and azides (‘’ ) and is a prime example for a novel class of sensor constructs. Especially, the attachment of two (different) fluorophores on opposite sides of the molecular rods paves the way to new sensor systems with less bulky (compared to the conventional DNA‐ or protein‐based concepts), nevertheless rigid spacer constructs, , for FRET‐based sensing applications. A detailed photophysical characterization was performed in MeOH (and in basic HO/MeOH mixtures) for ) rod constructs containing carboxyfluorescein, ) rod constructs containing carboxyrhodamine, ) rod constructs containing both carboxyfluorescein and carboxyrhodamine, and ) rod constructs containing both pyrene and perylene parts. For each dye (pair), two rod lengths with different numbers of spirane units were synthesized and investigated. The rod constructs were characterized in ensemble as well as single‐molecule fluorescence experiments with respect to ) specific roddye and ) dyedye interactions. In addition to MeOH and MeOH/NaOH, the rod constructs were also investigated in micellar systems, which were chosen as a simplified model for membranes.
    Keywords: Molecular Rods ; Resonance Energy Transfer Fret ; Carboxyfluorescein ; Carboxyrhodamine ; Pyrene ; Perylene ; Fluorescence
    ISSN: 0018-019X
    E-ISSN: 1522-2675
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  • 6
    Language: German
    In: Stahlbau, April 2012, Vol.81(S1), pp.159-170
    Description: Regeln der Technik und Normen dienen heute als Bemessungsgrundlage für den Einsatz von Glas als Konstruktionswerkstoff und gewährleisten damit, dass der spröde Werkstoff Glas mit einem für andere Baustoffe vergleichbaren Sicherheitsniveau bemessen werden kann. Ergänzt durch aktuelle Forschungsergebnisse, wie auch hier veröffentlicht, sind Anwendungen von Glas als Konstruktionswerkstoff umsetzbar, die an die Leichtigkeit der Konstruktion, für die z.B. die Gewächshäuser des 19. Jahrhunderts berühmt waren, wieder anknüpft. Über den Ansatz der Detaillierung bei Fügung und Profil werden zwei Projekte vorgestellt, die in engster Zusammenarbeit zwischen Architekt, Ingenieur und ausführender Firma entstanden. Dabei wurde der Einsatz von Glas als Konstruktionswerkstoff nicht nur im Hinblick auf seine statische Beanspruchbarkeit, sondern auch glasspezifische Fügetechniken weiter entwickelt.
    Keywords: Konstruktion Historischer Glashäuser ; Stabilisierung Durch Glas ; Glasbau ; Sonderkonstruktionen ; Bestandssicherung Und Erhaltung
    ISSN: 0038-9145
    E-ISSN: 1437-1049
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  • 7
    Language: English
    In: Journal of Facade Design and Engineering, 01 December 2013, Vol.1(1-2), pp.73-84
    Description: Acrylic glass is omnipresent in the industrialised world; but as a building material most architects, facade planners and engineers are still unfamiliar with this material. In most cases it is applied as a substitute for glass which leads to inappropriate joints and fixtures. During the years of the path toward the digital era, the authors were in the fortunate position to be involved in several unconventional glass and acrylic glass projects.On the basis of their most recent project, the  facade of the Vitra VSL Factory by SANAA Architekten, they describe the development of a facade for which they chose acrylic glass not as a substitute for glass but rather as a conscious material choice. Since the entire facade is it was possible to apply the manufacturing technology of deep-drawing, allowing for very thin wall thicknesses.
    Keywords: Engineering
    ISSN: 2213-302X
    E-ISSN: 2213-3038
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  • 8
    Language: German
    In: Stahlbau, April 2013, Vol.82(S1), pp.23-34
    Description: Acrylglas ist in der industrialisierten Welt allgegenwärtig, als Baumaterial ist es Architekten, Fassadenplanern und Ingenieuren dennoch fremd. Die Autoren konnten in den Jahren des digitalen Aufbruchs immer wieder unkonventionelle Glas‐ und Acrylglas‐Projekte auf der Ingenieurseite betreuen. Dieser Aufsatz soll anhand des jüngsten Projektes, der Fassade an der Vitra VSL Factory von SANAA Architekten aufzeigen, welche Potenziale das Material insbesondere im Zusammenwirken mit einer innovativen Klebetechnik hat. In the industrialized world, acrylic glass is omnipresent, however as material for building components it is rarely known by architects and engineers. During the years of the digital revolution, the authors could gather experience in engineering of numerous free form glass and acrylic facades and envelopes. By presentation of their work for the most recent project, the Vitra VSL Factory façade by SANAA Architects, the authors show the potential as well as the constraints of the work with silicone‐bonded acrylic glass.
    Keywords: Kunststoff‐Fassade ; Silikonklebung ; Acrylglas ; Plexiglas ; Pmma ; Vitra ; Sanaa ; Nkbak ; Imagine Structure ; Plastic‐Facade ; Silicone Bond ; Acrylic ; Plexiglas ; Pmma ; Facade Engineering ; Vitra ; Sanaa ; Nkbak ; Imagine Structure ; Entwurf Und Konstruktion ; Werkstoffe ; Bauwerkhülle
    ISSN: 0038-9145
    E-ISSN: 1437-1049
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  • 9
    Language: English
    In: Agronomy for Sustainable Development, 2017, Vol.37(6), pp.1-29
    Description: Global trends in demand for biomass-based food, feed, energy, and fiber call for a sustainable intensification of agricultural production. From the perspective of sustaining soil functions, this implies the integration of soil productivity with the other soil functions and services, namely carbon sequestration, water purification and retention, and nutrient and matter cycling as well as biodiversity. Soil management is the key to this integration. The proper anticipation of future opportunities and challenges for sustainable soil management requires an analysis of drivers and trends affecting soil management. Here, we review drivers and trends of soil management and their relevance for soil functions taking Germany as an example of industrialized agricultural systems with low yield gaps. We analyzed socio-economic, biophysical and technological drivers and identified two types of future management changes: (1) Quantitative changes, i.e., more or less of the same input factors, such as fertilizers, as part of a moderate intensification. (2) Qualitative changes: There, we found the strongest signals for the following practices: higher precision and lightweight machines triggered by information and communication technology (ICT) and robotics; diversification of crop rotations, including the integration of lignocellulosic crops; inoculation with biota; and new crop varieties. Positive practices may be reinforced by a behavioral trend towards sustainable soil management, driven by increasing awareness, knowledge, and consumer demand. They offer opportunities for relieving mechanical pressures from weight and contact stress, chemical pressures from pesticides and fertilizers and promoting soil biodiversity without compromising the soil’s production function. We also found threats, such as increased removal of organic residues and potentially harmful organisms. This foresight study is the first to delineate opportunities and challenges for sustainable soil management and intensification. It informs researchers who intend to improve the knowledge base for reinforcement of positive and mitigation of negative trends of soil management.
    Keywords: Foresight ; Soil management ; Sustainable intensification ; Drivers of agronomic trends ; Cropping systems
    ISSN: 1774-0746
    E-ISSN: 1773-0155
    Source: Springer Science & Business Media B.V.
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  • 10
    Language: English
    In: Phytochemistry, 2011, Vol.72, pp.21-26
    Description: Tripterygium wilfordii Hook.f., known as Leigongteng (Thunder God Vine) in traditional Chinese medicine, has attracted much attention for its applications in relieving autoimmune disorders such as rheumatoid arthritis and systemic lupus erythematosus, and for treating cancer. Molecular analyses of the ITS and 5S rDNA sequences indicate that T. hypoglaucum and T. doianum are not distinct from T. wilfordii, while T. regelii should be recognized as a separate species. The results also demonstrate potential value of rDNA sequence data in forensic detection of adulterants derived from Celastrus angulatus in commercial samples of Leigongteng. ; Includes references ; p. 21-26.
    ISSN: 0031-9422
    Source: AGRIS (Food and Agriculture Organization of the United Nations)
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