Format:
Online-Ressource (XI, 330p. 49 illus., 41 illus. in color, digital)
ISBN:
9783709104392
Series Statement:
SpringerLink
Content:
The induction of antigen-specific immune responses after in vivo transfection with expression plasmids has triggered a revolution of vaccine research. After a first hype, evoked by the fascinating options of this method, clinical studies did not reach the ambitious aims and a phase of disillusion ensued. It became obvious that Gene vaccines displayed a weaker immunogenicity in humans than had been observed in the mouse models. Meanwhile these hurdles have been overcome and gene vaccines undergo a renaissance. The present book gives an update of the "world of naked gene vaccines", nam
Note:
Includes bibliographical references
,
Gene Vaccines; Preface; Contents; Contributors; 1: General Mechanisms of Gene Vaccines; Introduction; General Properties of Naked DNA Vaccines; Vector Design; Removal of Prokaryotic Vector Elements; Transcriptional Elements; Antigen Design; Codon Optimization; Guided Translation; Delivery Modalities; In Vivo Electroporation; Gene Gun Immunization; Biojector 2000™; Other Delivery Methods; Mechanisms of In Vivo Uptake of Naked Gene Vaccines; Pharmacokinetics and Nuclear Import of Plasmid DNA; Adjuvanticity of Nucleic Acids: Immunostimulatory Properties of DNA and RNA
,
Toll-like Receptors (TLRs): Endosomal Sensors of RNA and DNARIG-I-like Receptors (RLRs): Cytosolic Sensors of RNA; Cytosolic Sensors of DNA; Impact on Immunogenicity of DNA and RNA Vaccines; Distribution and Presentation of Antigens Encoded by Gene Vaccines; Antigen Persistence; Antigen Distribution; Antigen Kinetics; Processing of Antigens Encoded by DNA Vaccines; MHC Class I Presentation; MHC Class II Presentation; Conclusions; References; 2: Strategies to Improve DNA Vaccine Potency: HPV-Associated Cervical Cancer as a Model System; Introduction
,
Antigen-specific Immunotherapy for the Treatment of CancersHPV-associated Cervical Cancer as a Model for Antigen-specific Cancer Immunotherapy; DNA Vaccines as an Attractive Vaccination Approach; Importance of Dendritic Cells for Potent Immune Responses; Strategies to Improve DNA Vaccine Potency Through Modification of DCs; Targeting DNA to DCs Through Different Delivery Methods; Gene Gun; Microencapsulation of Plasmid DNA; HPV Pseudovirions; Strategies to Facilitate Uptake of Antigen Encoded by DNA Vaccine into DCs; Linking Antigen to DC-Targeting Molecules; Intercellular Antigen Spread
,
Increase Release of Tumor Antigen from Transfected CellsPhysical Methods to Enhance Transfection Efficiency of DNA Vaccine; Employment of Methods to Improve Antigen Expression, Processing and Presentation in DCs; Enhancing Antigen Expression; Enhancing MHC Class I Presentation; Bypassing MHC Class I Processing; Enhancing MHC Class II Antigen Presentation; Enhancing MHC Class I and II Transcription; Vaccine-Potentiating Strategies to Enhance DC and T cell Interaction; Utilizing Adjuvants to Activate DCs; Enhancing Cytokine/Chemokine Production for DC and T cell Activation
,
Prolonging DC SurvivalModifying Immunosuppressive Microenvironment; Prolonging T Cell Survival; Utilizing CD4+ T Cell Help; Conclusion; References; 3: Immune-Activating Mechanisms of Replicase-Based DNA and RNA Vaccines and Their Role in Immune-Apoptosis; Introduction; Why Do DNA Vaccines Work?; Virus-Based Vaccine Platforms: Tapping into the Innate Immune Recognition of Viral PAMPs; Replicon RNA: The First Generation of Replicase-Based Nucleic Acid Vaccines; Replicon-DNA: The Second Generation of Replicase-Based Nucleic Acid Vaccines
,
Conventional vs. Replicase-Based Vectors: Comparing Apples to Oranges
Additional Edition:
ISBN 9783709104385
Additional Edition:
Buchausg. u.d.T. Gene vaccines Wien : Springer, 2011 ISBN 9783709104385
Additional Edition:
ISBN 3709104386
Language:
English
Subjects:
Biology
,
Medicine
Keywords:
DNS
;
Impfstoff
;
Impfstoff
;
Gen-Targeting
;
Aufsatzsammlung
DOI:
10.1007/978-3-7091-0439-2
URL:
Volltext
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