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  • 1
    Book
    Book
    New York :Humana Press,
    UID:
    almahu_BV035237875
    Format: XVI, 391 S. : , Ill., graph. Darst.
    Edition: 2. ed.
    ISBN: 978-1-6173-7938-3 , 978-1-934115-61-9
    Series Statement: Methods in molecular biology 507
    Note: Literaturangaben
    Language: English
    Subjects: Biology
    RVK:
    Keywords: DNS ; Methylierung ; Aufsatzsammlung ; Aufsatzsammlung
    URL: Cover
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Online Resource
    Online Resource
    New York, NY :Springer New York :
    UID:
    almahu_9949251279002882
    Format: 1 online resource (XVIII, 704 p. 102 illus., 67 illus. in color.)
    Edition: 3rd ed. 2018.
    ISBN: 1-4939-7481-5
    Series Statement: Methods in Molecular Biology, 1708
    Content: This third edition volume expands on the previous editions with a comprehensive update on the available technologies required to successfully perform DNA methylation analysis. The different technologies discussed in this book analyze the global DNA methylation contents, comprehensive analyses using various NGS based methods for genome-wide DNA methylation analysis, along with precise quantification of DNA methylation levels on single CpG positions. The chapters in this book are divided into 7 parts: an introduction to the field along with tips on study design and data analysis; global DNA methylation levels; genome-wide DNA methylation analysis; highly multiplexed target regions; locus-specific DNA methylation analysis; DNA methylation analysis of specific biological samples; and hydroxymethylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reag ents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, DNA Methylation Protocols, Third Edition is a valuable resource for postdoctoral investigators and research scientists who work with different aspects of genetics, and cellular and molecular biology, as well as clinicians who are involved in diagnostics or treatment of diseases with epigenetic components.
    Note: A Summary of the Biological Processes, Disease-Associated Changes and Clinical Applications of DNA Methylation.- Considerations for Design and Analysis of DNA Methylation Studies.- Quantification of Global DNA Methylation Levels by Mass Spectrometry.- Antibody Based Detection of Global Nuclear DNA Methylation in Cells, Tissue Sections and Mammaliam Embryos.- Whole-Genome Bisulfite Sequencing Using the Ovation® Ultralow Methyl-Seq Protocol.- Tagmentation-Based Library Preparation for Low DNA Input Whole Genome Bisulfite Sequencing.- Post-Bisulfite Adaptor Tagging for PCR-Free Whole-Genome Bisulfite Sequencing.- Multiplexed Reduced Representation Bisulfite Sequencing with Magnetic Bead Fragment Size Selection.- Low Input Whole-Genome Bisulfite Sequencing Using a Post-Bisulfite Adapter Tagging Approach.- Methyl-CpG Binding Domain Sequencing: MBD-seq.- The HELP-Based Assays.- Comprehensive Whole DNA Methylome Analysis by InDigital Restriction Enzyme Analysis of Methylation (DREAM).- Nucleosome Occupancy and Methylome Sequencing (NOMe-seq).- Bisulfite Sequencing of Chromatin Immunoprecipitated DNA (BisChIP-seq).- A Guide to Illumina BeadChip Data Analysis.- Microdroplet PCR for Highly-Multiplexed Targeted Bisulfite Sequencing.- Multiplexed DNA Methylation Analysis of Target Regions Using Microfluidics (Fluidigm) -- Large-Scale Targeted DNA Methylation Analysis Using Bisulfite Padlock Probes.- Targeted Bisulfite Sequencing Using the SeqCap Epi Enrichment System.- Multiplexed and Sensitive DNA Methylation Testing Using Methylation-Sensitive Restriction Enzymes MSRE-qPCR.- Quantitative DNA Methylation Analysis at Single-Nucleotide Resolution by Pyrosequencing®.- Methylation-Specific PCR.- Quantitation of DNA Methylation by Quantitative Multiplex Methylation-Specific PCR (QM-MSP) -- MethyLight and Digital MethyLight.- Quantitative Region-Specific DNA Methylation Analysis by the EPITYPERTM Technology.- Methylation-Specific Multiplex Ligation-Dependent Probe Amplification  (MS-MLPA).- Methylation - Sensitive - High Resolution Melting (MS-HRM).- Hairpin Bisulfite Sequencing: Synchronous Methylation Analysis on Complementary DNA Strands of Individual Chromosomes.- Helper-Dependent Chain Reaction (HDCR) for Selective Amplification of Methylated DNA Sequences.- DNA Methylation Analysis from Blood Spots: Increasing Yield and Quality for Genome-Wide and Locus-Specific Methylation.- DNA Methylation Analysis of Free-Circulating DNA in Body Fluids.- Tet-Assisted Bisulfite Sequencing (TAB-seq).- Multiplexing for Oxidative Bisulfite Sequencing (oxBS-seq).- Affinity-Based Enrichment Techniques for the Genome-Wide Analysis of 5-Hydroxymethylcytosine.
    Additional Edition: ISBN 1-4939-7479-3
    Language: English
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  • 3
    Online Resource
    Online Resource
    Totowa, NJ :Humana Press :
    UID:
    almahu_9949251523502882
    Format: 1 online resource (XVI, 392 p.)
    Edition: 2.
    ISBN: 1-59745-522-9
    Series Statement: Methods in Molecular Biology, 507
    Uniform Title: DNA methylation protocols.
    Content: Over the past few years, DNA methylation technologies and our knowledge of DNA methylation patterns have been advancing at a breathtaking pace. Due to this fact, DNA Methylation: Methods and Protocols, Second Edition completely revises, updates, and expands upon the popular first edition with the most current novel techniques, easier to use and more refined by the tested experience of leading experts. This revision reflects contemporary study of the subject: the analysis of gene-specific DNA methylation patterns has been complemented by genome-wide approaches, and epigenomics takes a central place. Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume present brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, DNA Methylation: Methods and Protocols, Second Edition is the perfect resource for scientists who wish to further our understanding of the molecular processes that determine the genomic DNA methylation landscape and for those who aim to identify and implement DNA methylation-based biomarkers for clinical and diagnostic applications.
    Note: Bibliographic Level Mode of Issuance: Monograph , DNA Methylation: An Introduction to the Biology and the Disease-Associated Changes of a Promising Biomarker -- Global Methylation Levels -- Quantification of Global DNA Methylation by Capillary Electrophoresis and Mass Spectrometry -- Methyl Group Acceptance Assay for the Determination of Global DNA Methylation Levels -- Methods For Whole Genome Analysis Of Dna Methylation Patterns -- Immunodetection Array -- Methylated DNA Immunoprecipitation (MeDIP) -- The MIRA Method for DNA Methylation Analysis -- The Help Assay -- Differential Methylation Hybridization: Profiling DNA Methylation with a High-Density CpG Island Microarray -- Analysis of DNA Methylation by Amplification of Intermethylated Sites (AIMS) -- Methylation-Sensitive Representational Difference Analysis (MS-RDA) -- Restriction Landmark Genomic Scanning: Analysis of CpG Islands in Genomes by 2D Gel Electrophoresis -- GoldenGate® Assay for DNA Methylation Profiling -- 5?-Azacytidine Expression Arrays -- Gene-Specific Methylation Analysis -- DNA Methylation Analysis by Bisulfite Conversion, Cloning, and Sequencing of Individual Clones -- Identification and Quantification of Differentially Methylated Loci by the Pyrosequencing™ Technology -- Mass Spectrometric Analysis of Cytosine Methylation by Base-Specific Cleavage and Primer Extension Methods -- Melting Curve Assays for DNA Methylation Analysis -- Methylation SNaPshot: A Method for the Quantification of Site-Specific DNA Methylation Levels -- Bio-COBRA: Absolute Quantification of DNA Methylation in Electrofluidics Chips -- Restriction Digestion and Real-Time PCR (qAMP) -- MethylQuant: A Real-Time PCR-Based Method to Quantify DNA Methylation at Single Specific Cytosines -- Methylation-Specific PCR -- MethyLight -- Quantification of Methylated DNA by HeavyMethyl Duplex PCR -- Special Applications -- Analysis of Methylated Circulating DNA in Cancer Patients’ Blood -- Prevention of PCR Cross-Contamination by UNG Treatment of Bisulfite-Treated DNA -- Profiling DNA Methylation from Small Amounts of Genomic DNA Starting Material: Efficient Sodium Bisulfite Conversion and Subsequent Whole-Genome Amplification. , English
    Additional Edition: ISBN 1-934115-61-4
    Language: English
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  • 4
    Online Resource
    Online Resource
    New York, NY :Springer New York :
    UID:
    almahu_9949251690902882
    Format: 1 online resource (XIV, 414 p. 83 illus., 57 illus. in color.)
    Edition: 2nd ed. 2015.
    ISBN: 1-4939-2715-9
    Series Statement: Methods in Molecular Biology, 1315
    Uniform Title: Pyrosequencing protocols.
    Content: The primary purpose of this volume is to demonstrate the range of applications of the Pyrosequencing technology in research and diagnostics and to provide detailed protocols. Beginning with an up-to-date overview of the biochemistry, the volume continues with quantitative analysis of genetic variation, ratio of expressed alleles at the RNA level, analysis of DNA methylation, global DNA methylation assays, specialized applications for DNA methylation analysis including loss of imprinting, single blastocyst analysis, allele-specific DNA methylation patterns, DNA methylation patterns associated with specific histone modifications. The volume further details tools and protocols for the detection of viruses and bacteria, and genetic and epigenetic analyses for forensics using Pyrosequencing. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.   Comprehensive and adaptable, Pyrosequencing: Methods and Protocols, Second Edition will greatly aid doctorial students, postdoctoral investigators, and research scientists studying different aspects of genetics and cellular and molecular biology.
    Note: Bibliographic Level Mode of Issuance: Monograph , The History of PyrosequencingÒ -- Pyro Mark® Instruments, Chemistry and Software for Pyrosequencing® Analysis -- Software-Based Pyrogram® Evaluation -- Quantitative Validation and Quality Control of Pyrosequencing® Assays -- Extended KRAS and NRAS Mutation Profiling by Pyrosequencing® -- Universal BRAF State Detection by the Pyrosequencing®-based U‑BRAFV600 Assay -- Pyrosequencing®-based Identification of Low Frequency Mutations Enriched through Enhanced-ice-COLD-PCR -- Analysis of Mutational Hotspots in Routinely Processed Bone Marrow Trephines by Pyrosequencing® -- Analysis of Copy Number Variation By Pyrosequencing® using Paralogous Sequences -- Prenatal Diagnosis of Chromosomal Aneuploidies by Quantitative Pyrosequencing® -- HLA-B and HLA-C Supratyping by Pyrosequencing® -- Allele Quantification Pyrosequencing® at Designated SNP sites to Detect Allelic Expression Imbalance and Loss-of-heterozygosity -- Quantitative DNA Methylation Analysis by Pyrosequencing® -- ­Quantitative Methylation Analysis of the PCDHB Gene Cluster -- Assessment of Changes in global DNA Methylation Levels by Global Analysis of DNA 5-methylcytosine using the Luminometric Methylation Assay, LUMA -- Limiting Dilution Bisulfite Pyrosequencing®: a Method for Methylation Analysis of Individual DNA Molecules in a Single or a Few Cells -- Detection of Loss of Imprinting by Pyrosequencing® -- Analysis of DNA Methylation Patterns in Single Blastocysts by Pyrosequencing® -- Allele-specific DNA methylation detection by Pyrosequencing® -- SNP-based Quantification of Allele-Specific DNA Methylation Patterns by Pyrosequencing® -- DNA Methylation Analysis of ChIP Products at Single Nucleotide Resolution byPyrosequencing® -- Multiplex Pyrosequencing®: Simultaneous Genotyping Based on SNPs from Distant Genomic Regions -- Detection of Drug-Resistant Mycobacterium tuberculosis -- Application of Pyrosequencing® in Food Biodefense -- Forensic Analysis of Mitochondrial and Autosomal Markers using Pyrosequencing® -- Tissue-specific DNA Methylation Patterns in Forensic Samples Detected By Pyrosequencing®. , English
    Additional Edition: ISBN 1-4939-2714-0
    Language: English
    Keywords: Laboratory Manuals. ; Laboratory manuals. ; Laboratory Manuals. ; Laboratory manuals. ; Laboratory Manuals. ; Laboratory manuals. ; Laboratory Manuals. ; Laboratory manuals.
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  • 5
    Book
    Book
    Norfolk :Caister Acad. Press,
    UID:
    almahu_BV025514054
    Format: XI, 404, A-3 S. : , Ill., graph. Darst.
    ISBN: 978-1-904455-23-3
    Language: English
    Subjects: Biology
    RVK:
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  • 6
    Book
    Book
    New York, NY :Humana Press,
    UID:
    almahu_BV045030068
    Format: xviii, 704 Seiten : , Illustrationen, Diagramme.
    Edition: Third edition
    ISBN: 978-1-4939-7479-5
    Series Statement: Methods in molecular biology 1708
    Additional Edition: Erscheint auch als Online-Ausgabe ISBN 978-1-4939-7481-8
    Language: English
    Subjects: Biology
    RVK:
    Library Location Call Number Volume/Issue/Year Availability
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  • 7
    Book
    Book
    New York :Humana Press,
    UID:
    almahu_BV042753500
    Format: xiv, 414 S. : , Illustrationen, Diagramme.
    Edition: Second edition
    ISBN: 978-1-4939-2715-9 , 978-1-4939-2714-2
    Series Statement: Methods in molecular biology 1315
    Additional Edition: Erscheint auch als Online-Ausgabe 10.1007/978-1-4939-2714-2
    Language: English
    Subjects: Biology
    RVK:
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