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Berlin Brandenburg

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  • 1
    In: KnE Life Sciences, 09/20/2015, Vol.2(1), p.129
    ISSN: KnE Life Sciences
    E-ISSN: 2413-0877
    Source: CrossRef
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  • 2
    In: KnE Life Sciences, 09/20/2015, Vol.2(1), p.154
    ISSN: KnE Life Sciences
    E-ISSN: 2413-0877
    Source: CrossRef
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  • 3
    Language: English
    In: Indian Journal of Physiotherapy and Occupational Therapy - An International Journal, 2018, Vol.12(4), p.202
    ISSN: 0973-5666
    E-ISSN: 0973-5674
    Source: CrossRef
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  • 4
    Language: English
    In: Indonesian Journal of Biotechnology, 01 June 2015, Vol.20(1), pp.69-76
    Description: Anthocyanin of pigmented rice inhibits the growth of cancer cells. The cytotoxicity and apoptosisinducing properties of local black rice (cv Cempo Ireng) extracts and fractions, which contain anthocyanin including cyanidin 3-glucoside and peonidin 3-glucoside, on human cervical cancer cell line (HeLa cells) has been evaluated. The pigmented rice bran was extracted and fractionated using methanol-HCl. The MTT test was performed on HeLa cell cultures to observe the IC50 value. Preparative TLC was performed to obtain the fractions of black rice bran. Cyanidin 3-glucoside and peonidin 3-glucoside were identified in the pigmented rice bran extract and fractions using UHPLC. Flowcytometry analysis was performed to measure the percentage of apoptotic cells. Our results suggest that the fractions are more toxic than the methanolic crude extract with IC 50 values of 85.95 ± 5.56 μg/mL (the lowest one) and 408.13 ± 51.9 μg/mL, respectively. The concentration of cyanidin 3-glucoside and peonidin 3-glucoside in the methanolic extract were 1.89 and 0.84 μg/mg, respectively. The apoptosis induction by fractions F2 and F4 (52 and 55%) were significantly higher compared to fraction F3 and F5 (30 and 33%) and doxorubicin (21%). Cyanidin 3-glucoside was detected in F4 (0.14 μg/ml) while peonidin 3-glucoside in F2 (0.012 μg/ml), however both were not detected in F3 and F5.
    Keywords: Cempo Ireng ; Cyanidine 3-Glucoside ; Peonidin 3-Glucoside ; Apoptosis ; Hela Cells ; Engineering
    ISSN: 0853-8654
    E-ISSN: 2089-2241
    Source: Directory of Open Access Journals (DOAJ)
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  • 5
    Language: English
    In: Procedia Chemistry, 2015, Vol.14, pp.465-468
    Description: Previous study showed that kaffir lime leaf contains alkaloid, flavonoid, terpenoid, tannin and saponin. The objective of this study was to examine the cytotoxic effect of kaffir lime leaf extract on cervical cancer and neuroblastoma cell lines. The method used for this research to determine cell viability was an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results showed that an ethyl acetate extract had an IC50 for HeLa cells, UKF-NB3, IMR-5 and SK-N-AS parental cells of 40.7 μg · mL , 28.4 μg · mL , 14.1 μg · mL , and 25.2 μg · mL respectively. Furthermore, the IC50 of chloroform extracts for HeLa cells, UKF-NB3, IMR-5 and SK-N-AS parental were 17.6 μg · mL , 18.9 μg · mL , 6.4 μg · mL , and 9.4 μg · mL respectively. These data showed that kaffir lime extract reduces the viability of cervical and neuroblastoma cell lines and may have potential as anti-cancer compounds.
    Keywords: Cervical Cancer ; Kaffir Lime ; Mtt Assay ; Neuroblastoma ; Chemistry
    ISSN: 1876-6196
    E-ISSN: 1876-6196
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  • 6
    Language: English
    In: Biochemical and Biophysical Research Communications, 02 December 2016, Vol.481(1-2), pp.159-164
    Description: In cattle breeding, co-culture with granulosa cells (GCs) is one of the strategies to improve oocyte maturation and fertilization potential, but yields are still suboptimal due to GC apoptosis. We previously set up an co-culture system of cumulus-oocyte-complexes (COCs) anchored to GC multilayers adhering to the basal lamina (COCGs), in which GC apoptosis was inhibited by FSH supplementation. Here, we assessed the antiapoptotic effect of EGF (5 ng/ml-EGF5) alone or in synergism to FSH (50mU/ml-FSH50) on pig COCGs. COCG morphology, apoptotic rate, procaspase-8 and-9 expression levels and surface ultrastructure were determined. Results showed an increased % of apoptotic GCs in control and EGF5 (≈80%) respect to sampling (≈3%) and caspase-8 and -9 activation. In contrast, apoptotic cells were significantly reduced by FSH50 (≈35%) supplementation, with inactive Procaspase-8 and -9 highly expressed. The pro-survival effect of FSH was strengthened by EGF (EGF5+FSH50), as evidenced by a significant reduction of apoptosis (≈15%) and high expression levels of Procaspase-8 and -9. Ultrastructural analysis revealed that GC multilayers were characterized by round-to-ovoid cells connected each other and to the basal lamina by cytoplasmic projections. Microvilli shortening/thickening/reduction, cytoplasmic projection rarefaction, blebbing of apoptotic bodies and degenerating/atresic GCs were observed in control and EGF5 groups. FSH50 induced the formation of an abundant mucinous matrix, due to granulosa expansion. Blebs and atresic areas were rarely observed. In EGF5+FSH50 group, GCs were well-preserved, richly covered by microvilli and connected by numerous cytoplasmic projections. Degenerative phenomena were rarely observed. In conclusion, EGF in synergism with FSH seems to better counteract GC apoptosis in a co-culture of pig GC multilayers.
    Keywords: Granulosa Cells ; Apoptosis ; Pig ; Scanning Electron Microscopy ; Co-Culture System ; Biology ; Chemistry ; Anatomy & Physiology
    ISSN: 0006-291X
    E-ISSN: 1090-2104
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  • 7
    Language: English
    In: Indonesian Journal of Biotechnology, 01 June 2017, Vol.22(1), pp.49-54
    Description: The chemical contents and health benefits of black rice bran of some rice cultivars have been investigated. However, there has been little research on the ‘Cempo Ireng’ cultivar from Sleman, Yogyakarta. The aim of this present study was to determine the anthocyanin, antioxidant activity, and macro- and micronutrients contents of black rice bran from this local cultivar. The anthocyanin in the black rice bran was extracted using the maceration method with methanol as a solvent. The extract obtained was separated through a preparative thin layer chromatography (TLC) of silica GF254 and a mobile phase composed of n-butanol, acetic acid, and water. Two fractions were collected and analyzed for the anthocyanin content. The preparative TLC spots were separated for further detection and measurement of cyanidin 3-O-glucoside using HPLC followed by LC-MS. The antioxidant activity of the fractions were measured using the DPPH free radical scavenging method. The results showed that the anthocyanin in fraction 1 was identified as cyanidin 3-O-glucoside (66.1 ± 10.6 µg/g). The IC50 of fractions 1 and 2 were 200.96 and 218.36 µg/mL, respectively. Analysis of the macro- and micronutrients revealed that the black rice bran of ‘Cempo Ireng’ had nutrient contents comparable with other rice cultivars. Therefore, this local black rice bran can be used as a source of antioxidants and macro-- and micronutrients.
    Keywords: Nthocyanin ; Antioxidant ; Black Rice Bran ; Nutrient Contents ; Engineering
    ISSN: 0853-8654
    E-ISSN: 2089-2241
    Source: Directory of Open Access Journals (DOAJ)
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  • 8
    Language: English
    In: Indonesian Journal of Biotechnology, 01 June 2015, Vol.20(1), pp.77-87
    Description: Cellulase has been widely used as biocatalyst in industries. Production of cellulase from microorganisms has many advantages such as short production time and less expense. Our previous study indicated that one of cellulolytic bacteria from digestive tract of milkfish (Chanos chanos), namely BSA B1, showed the highest cellulase activity. The objective of this study was to determine the phylogenetic of BSA B1 strain using 16S rRNA gene sequence. Furthermore, this study also determine the specific activity of purified cellulase from BSA B1 strain and its potency to hydrolyze Chlorella zofingiensis cellulose. Cellulase was purified using ammonium sulphate precipitation, dialysis, and ion exchange chromatography. The purified cellulase was used to hydrolyze cellulose of C. zofingiensis. The result demonstrated that BSA B1 strain was closely related with Bacillus aerius and Bacillus licheniformis. The specific activity of the crude enzyme was 1.543 U mL-1; after dialysis was 4.384 U mL-1; and after chromatography was 7.543 U mL-1. Purified cellulase exhibited activity in hydrolyzed both CMC and C. zofingiensis. Compared to commercial cellulase, purified cellulase had lower activity in hydrolyzed CMC but higher activity in hydrolyzed C. zofingiensis. Ethanol dehydration could potentially increase the reducing sugar yield in cellulose hydrolysis when used appropriately. Morphology of C. zofingiensis cell has changed after incubation with cellulases and ethanol dehydration indicated degradation of cell wall.
    Keywords: Cellulase ; Enzyme Purification ; Cellulose Hydrolysis ; BSA-B1 ; Engineering
    ISSN: 0853-8654
    E-ISSN: 2089-2241
    Source: Directory of Open Access Journals (DOAJ)
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  • 9
    Language: English
    In: Biochemical and Biophysical Research Communications, 2009, Vol.382(1), pp.160-164
    Description: We studied the ability of hyaluronan (HA) to inhibit apoptosis in porcine granulosa cells. The granulosa layer with cumulus–oocyte complex is cultured in media supplemented with follicle stimulating hormone (FSH) and 4-MU an inhibitor of hyaluronan synthases. The concentration of HA significantly increased after supplemented with FSH, but significantly decreased with 4-MU. CD44, receptor of HA, expressed after cultured with FSH, decreased in addition low concentration of 4-MU, whereas not detected in high concentration of 4-MU, indicating parallel relation between the amount of HA and CD44 expression. The 4-MU treatment also decreased the expression of procaspase-3, -8, -9 suggesting that inhibition of HA synthesis leads to activation of these caspases. Moreover, addition of anti-CD44 antibody decreased the expression of procaspases suggesting that perturbation of HA–CD44 binding leads activation of caspases. Hence, HA has ability to inhibit apoptosis and HA–CD44 binding is important on apoptosis inhibitory mechanism in porcine granulosa cells.
    Keywords: Hyaluronan ; Cd44 ; FSH ; Caspase ; Apoptosis of Granulosa Cells ; Biology ; Chemistry ; Anatomy & Physiology
    ISSN: 0006-291X
    E-ISSN: 1090-2104
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  • 10
    Language: English
    In: Journal of Biological Researches, 01 December 2016, Vol.22(1), pp.1-6
    Description: The use of NAPZA (Narcotics, Alcohol, Psychotropic, and other addictive substances) in Indonesia keeps increasing. One type of frequently used NAPZAs is diazepam. Diazepam is a kind of sedative-hypnotics drug which belongs to benzodiazepine. The objective of this study was to determine kidney function by examining the level of urine ureum and creatinine also histological structure in rat after treated with diazepam. Rats were divided into one control group and three diazepam treatment groups which were solvent control (PEG 1%), treatment I (62.25 mg/kg BW), treatment II (83 mg/kg BW), and treatment III (124.5 mg/kg BW) of diazepam. These doses were based on LD50 in human. Rat urine was taken on the D0, D7, D14, D21, and D28. The ureum level was examined by ureumse-GLDH method and the creatinine level was analyzed by Jaffe method. The histological section was made by paraffin method with Hematoxylin and Eosin (HE) staining. Results showed that diazepam given to rat for 28 days led the increase of urine ureum and creatinine levels compare to control group. Furthermore, histological damages of the kidney also detected including cellular injuries, both reversible and irreversible injuries, congestion, hemorrhage, and glomerular damage. In conclusion, the use of diazepam for 28 days affected kidney function in rat. Key words: diazepam, ureum, creatinine, kidney.
    Keywords: Animal Physiology ; Biology
    ISSN: 0852-6834
    E-ISSN: 2337-389X
    Source: Directory of Open Access Journals (DOAJ)
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