Format:
Online-Ressource (XXV, 604 p. 117 illus., 43 illus. in color, digital)
ISBN:
9781461423560
,
1283933535
,
9781283933537
Series Statement:
Fungal Biology
Content:
Laboratory Protocols in Fungal Biology presents the latest techniques in fungal biology. This book analyzes information derived through real experiments, and focuses on cutting edge techniques in the field. The book comprises 57 chapters contributed from internationally recognised scientists and researchers. Experts in the field have provided up-to-date protocols covering a range of frequently used methods in fungal biology. Almost all important methods available in the area of fungal biology viz. taxonomic keys in fungi; histopathological and microscopy techniques; proteomics methods; genomics methods; industrial applications and related techniques; and bioinformatics tools in fungi are covered and complied in one book. Chapters include introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting. Each chapter is self-contained and written in a style that enables the reader to progress from elementary concepts to advanced research techniques. Laboratory Protocols in Fungal Biology is a valuable tool for both beginner research workers and experienced professionals.
Note:
Description based upon print version of record
,
Laboratory Protocols in Fungal Biology; Foreword; Foreword; Preface; Contents; Contributors; 1: Safety Norms and Regulations in Handling Fungal Specimens; Introduction; Biosafety Levels; Materials (See Note 1); Methods; Routine Precautions When Working with Fungal Cultures; Experimentation; Accidents; Emergency Preparedness and Response; Information and Training; Notes; References; 2: Methods of Cryopreservation in Fungi; Introduction; Perlite Protocol (PP); Materials; Methods; Strains (Cultures); Culture Preparation and Freezing-Thawing Protocols; Viability Test
,
Growth Estimation and Morphological AnalysisReferences; 3: Long-Term Preservation of Fungal Cultures in All-Russian Collection of Microorganisms (VKM): Protocols and Results; Introduction; Cryopreservation of Filamentous Fungi; Freeze-Drying of Filamentous Fungi; Drying in Sterile Soil of Filamentous Fungi; Protocols; Protocol of Cryopreservation; Preparation of Cryovials (2.0 mL Externally Threaded Polypropylene, Nunc, Denmark); Preparation of Cryoprotectant: 10% (v/v) Glycerol; Preparation of Cultures; Filling of Vials; Fast Cooling Rates Regime of Freezing (~400 grad/min)
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Programmed Regime of FreezingThe First Protocol; The Second Protocol; Thawing; Control of Viability; Protocol of Freeze-Drying; Preparation of Ampoules; Preparation of Lyoprotectant Agent; Preparation of Cultures; Filling of Ampoules; The First Stage (Primary Drying); Preparation of Ampoules for the Second Stage of Freeze-Drying; The Second Stage (Secondary Drying); Vacuum Control; Control of Culture Viability; Storage; Protocol of Drying in Sterile Soil; Preparation of Sterile Soil; Preparation of Cultures; Soil Inoculation; Control of Viability; Result; Conclusion; References
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4: Fungal Specimen Collection and ProcessingIntroduction; Sample Collection; Materials; Methods; Sample Preparation; Sample Dilution; Preparing Plates; Culturing; Spread Plate; Results; Identi fi cation; Isolate a Pure Culture; Direct Culture Methods; Storage of Fungi; Storage in Water; Cold Temperature; Under Oil; Freeze Drying or Lyophilization; Low-Temperature Storage; References; 5: Chemical and Molecular Methods for Detection of Toxigenic Fungi and Their Mycotoxins from Major Food Crops; Introduction; Some Important Mycotoxins; A fl atoxins ( Aspergillus spp.); Ochratoxin; Fumonisins
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DeoxynivalenolMaterials; DNA Extraction; Basic Equipment Required for Polymerase Chain Reaction; General PCR Conditions; Materials Required for Chromatography-Based Method; Methods; DNA Fingerprinting Methods; Extraction of DNA from Pure Cultures of Fungi [ 18 ]; Extraction of DNA from Contaminated Food Samples (e.g., Maize); Polymerase Chain Reaction; Polymerase Chain Reaction-Based Detection of A fl atoxigenic Fungal Species; Polymerase Chain Reaction-Based Detection of Ochratoxigenic Fungi; Polymerase Chain Reaction-Based Detection of Tricothecene-Producing Fusarium Species
,
Polymerase Chain Reaction-Based Detection of Fumonisins Producing Fusarium Species
Additional Edition:
ISBN 9781461423553
Additional Edition:
Buchausg. u.d.T. Laboratory protocols in fungal biology New York : Springer, 2013 ISBN 1461423554
Additional Edition:
ISBN 9781461423553
Language:
English
Subjects:
Biology
DOI:
10.1007/978-1-4614-2356-0
URL:
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