Format:
Online-Ressource
ISSN:
1942-4434
Content:
Purpose: To study the effects of different chemically modified titanium surfaces on the proliferation, differentiation, adhesion, and apoptosis of osteoblast-like SaOS-2 cells. Materials and Methods: In this work, six different titanium materials were tested and compared to each other: (1) glazed; (2) unglazed; (3) unglazed and alkali-etched; (4) unglazed, sandblasted, acid- and alkali-etched; (5) unglazed and coated with zirconium nitride; and (6) unglazed, sandblasted, and acid-etched. The production of alkaline phosphatase (ALP), tumor necrosis factor alpha (TNF-α), matrix metalloproteinase-2, and the expression of adhesion proteins (integrin α3β1, vinculin) were evaluated using ELISA. Finally, the apoptosis of cells was analyzed by flow cytometry. Results: The most significant differences were found for unglazed sandblasted acid- and alkali-etched titanium discs compared with unglazed titanium discs. The production of TNF-α was decreased after 24 hours, as was the production of ALP after 72 hours. In contrast, the expression of integrin α3β1 was increased after 6 hours. None of the titanium discs showed an apoptotic effect on cells. Conclusions: This study has shown that physical surface treatments (such as surface roughness) play a more important role than chemical modifications. Generally, chemical modifications such as acid- and alkali-etching can affect the wettability of titanium surfaces, making a surface hydrophilic or hydrophobic according to the modification. The cell attachment is better on hydrophilic surfaces, while hydrophilic surfaces may slightly decrease the expression of ALP activity.
In:
volume:28
In:
number:5
In:
year:2013
In:
pages:1386-1394
In:
The international journal of oral & maxillofacial implants, Carol Stream, Ill. : Quintessence Publ. Co., 1986-, 28, Heft 5 (2013), 1386-1394, 1942-4434
Language:
English
URN:
urn:nbn:de:101:1-2023051909575672034762
URL:
https://doi.org/10.11607/jomi.3039
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2023051909575672034762
URL:
https://d-nb.info/1289848165/34
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