Format:
Online-Ressource
ISSN:
1615-9314
Content:
Abstract: Two chromatographic methods, high‐performance TLC (HPTLC) and HPLC, were developed and used for separation and quantitative determination of chlorogenic acid in green coffee bean extracts. For HPTLC silica gel Kieselgel 60 F 254 plates with ethyl acetate/dichlormethane/formic acid/acetic acid/water (100:25:10:10:11, v/v/v/v/v) as mobile phase were used. Densitometric determination of chlorogenic acid by HPTLC was performed at 330 nm. A gradient RP HPLC method was carried out at 330 nm. All necessary validation tests for both methods were developed for their comparison. There were no statistically significant differences between HPLC and HPTLC for quantitative determination of chlorogenic acid according to the test of equality of the means.
In:
volume:31
In:
number:2
In:
year:2008
In:
pages:237-241
In:
extent:5
In:
Journal of separation science, Weinheim : Wiley-VCH, [2000]-, 31, Heft 2 (2008), 237-241 (gesamt 5), 1615-9314
Language:
English
DOI:
10.1002/jssc.200700472
URN:
urn:nbn:de:101:1-2023080205070678585013
URL:
https://doi.org/10.1002/jssc.200700472
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2023080205070678585013
URL:
https://d-nb.info/1297954408/34
URL:
https://doi.org/10.1002/jssc.200700472
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