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  • 1
    Online Resource
    Online Resource
    New York, NY :Springer New York :
    UID:
    almahu_9949251323802882
    Format: 1 online resource (XI, 271 p. 27 illus., 20 illus. in color.)
    Edition: 1st ed. 2015.
    ISBN: 1-4939-2848-1
    Series Statement: Methods in Molecular Biology, 1330
    Content: This volume provides an understanding of the factors involved in nuclear reprogramming, which is essential for the success of reprogramming. The book is aimed at reprogramming differentiated cells and germ line transmission of pluripotent stem cells, and features chapters that deal with reprogramming-related issues such as analysis of mitochondrial DNA in reprogrammed cells and the isolation of reprogramming intermediates;  alternative methods for nuclear transfer; the production of germ-line chimeras from embryonic stem cells and induced pluripotent stem cells; and neonatal care and management of somatic cell nuclear transfer derived offspring.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell Reprogramming: Methods and Protocols is the perfect guide for molecular biologists, stem cell biologists, clinicians, biotechnologists, students, veterinarians, and animal care technicians involved with reprogramming, nuclear transfer, and transgenesis.
    Note: Bibliographic Level Mode of Issuance: Monograph , Cellular Reprogramming in Basic and Applied Biomedicine: The Dawn of Regenerative Medicine -- Synthetic mRNA Reprogramming of Human Fibroblast Cells -- MicroRNA Mediated Reprogramming of Somatic Cells into Induced Pluripotent Stem Cells -- Generation of Footprint-Free Induced Pluripotent Stem Cells from Human Fibroblasts Using Episomal Plasmid Vectors -- Reprogramming of Human Fibroblasts with Non-Integrating RNA Virus on Feeder-Free or Xeno-Free Conditions -- Inducing Pluripotency in Cattle -- Generation of Induced Pluripotent Stem Cells (iPSCs) From Adult Canine Fibroblasts -- Derivation of Equine-Induced Pluripotent Stem Cell Lines Using a PiggyBac Transposon Delivery System and Temporal Control of Transgene Expression -- Generation of Induced Pluripotent Stem Cells from Mammalian Endangered Species -- Generation of Efficient Germ-Line Chimeras Using Embryonic Stem Cells -- Generation of Viable Mice from Induced Pluripotent Stem Cells (iPSCs) Through Tetraploid Complementation -- Cloning Endangered Felids by Interspecies Somatic Cell Nuclear Transfer -- Generation of Chimeras from Porcine Induced Pluripotent Stem Cells -- A Novel Method on Somatic Cell Nuclear Transfer with the Minimum Equipment -- Neonatal Care and Management of Foals Derived by Somatic Cell Nuclear Transfer -- Isolation of Reprogramming Intermediates During Generation of Induced Pluripotent Stem Cells from Mouse Embryonic Fibroblasts -- Analysis of Mitochondrial DNA in Induced Pluripotent and Embryonic Stem Cells -- Genome Modification of Pluripotent Cells By Using Transcription Activator-Like Effector Nucleases (TALENs). , English
    Additional Edition: ISBN 1-4939-2847-3
    Language: English
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  • 2
    Online Resource
    Online Resource
    Totowa, NJ :Humana Press :
    UID:
    almahu_9949251272202882
    Format: 1 online resource (352 p.)
    Edition: 1st ed. 2006.
    ISBN: 1-280-83244-4 , 9786610832446 , 1-59745-154-1
    Series Statement: Methods in Molecular Biology, 348
    Content: Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The authors provide readily reproducible techniques for the generation of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer. Of immense benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones.
    Note: Description based upon print version of record. , Nuclear Transfer -- Origin and Progress of Nuclear Transfer in Nonmammalian Animals -- In Vitro Maturation and Embryo Production in Cattle -- Activation of Oocytes After Nuclear Transfer -- Culture Medium Preferences of Pre-Implantation Cloned Mouse Embryos -- Maintenance of Pregnancy in Pigs With Limited Viable Embryos -- Health and Neonatal Care of Bovine Clones -- Optimization of Procedures for Cloning by Somatic Cell Nuclear Transfer in Mice -- Production of Cloned Calves Using Roscovitine-Treated Adult Somatic Cells as Donors -- A Double Nuclear Transfer Technique for Cloning Pigs -- Nuclear Transfer in Nonhuman Primates -- Cloning of Exotic/Endangered Species: Desert Bighorn Sheep -- Handmade Somatic Cell Cloning in Cattle -- Genetic Modification of Sheep by Nuclear Transfer With Gene-Targeted Somatic Cells -- Transgenesis Using Nuclear Transfer in Goats -- Reprogramming Nuclei -- Nuclear Reprogramming -- Expression of Imprinted Genes in Cloned Mice -- Nuclear Remodeling Assay in Xenopus Egg Extract -- In Vitro Reprogramming of Nuclei and Cells -- Techniques for Nuclear Transfer to Mouse Embryonic Stem Cells -- Gene Expression and Methylation Patterns in Cloned Embryos -- Concluding Remarks -- Are There Any Normal Clones? -- Future and Applications of Cloning. , Also available on World Wide Web. , English
    Additional Edition: ISBN 1-58829-280-0
    Language: English
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  • 3
    Book
    Book
    New York :Humana Press,
    UID:
    almahu_BV042613975
    Format: XI,271 Seiten : , Illustrationen, Diagramme.
    ISBN: 978-1-4939-2847-7
    Series Statement: Methods in molecular biology 1330
    Additional Edition: Erscheint auch als Online-Ausgabe ISBN 978-1-4939-2848-4
    Language: English
    Subjects: Biology
    RVK:
    Keywords: Zelle ; Zelldifferenzierung ; Pluripotenz ; Methode ; Aufsatzsammlung
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  • 4
    Book
    Book
    Totowa, NJ : Humana Press
    UID:
    gbv_506703312
    Format: XI, 337 S. , Ill., graph. Darst.
    ISBN: 1588292800
    Series Statement: Methods in molecular biology 348
    Note: Includes bibliographical references and index , Origin and progress of nuclear transfer in nonmammalian animals / Marie A. Di BerardinoIn vitro maturation and embryo production in cattle / Marc-André Sirard and Karine Coenen -- Activation of oocytes after nuclear transfer / Zoltán Macháty -- Culture medium preferences of pre-implantation cloned mouse embryos / Björn Heindryckx, Josiane Van der Elst, and Marc Dhont -- Maintenance of pregnancy in pigs with limited viable embryos / Tim King and Paul A. De Sousa -- Health and neonatal care of bovine clones / Jacqui T. Forsyth and David N. Wells -- Optimization of procedures for cloning by somatic cell nuclear transfer in mice / Young Gie Chung, Shaorong Gao, and Keith E. Latham -- Production of cloned calves using roscovitine-treated adult somatic cells as donors / Kazuchika Miyoshi, Sezen Arat, and Steven L. Stice -- A double nuclear transfer technique for cloning pigs / Irina A. Polejaeva, Shawn Walker, and Keith Campbell -- Nuclear transfer in non-human primates / Shoukhrat M. Mitalipov and Don P. Wolf , Cloning of exotic/endangered species : desert bighorn sheep / James "Buck" Williams ... [et al.]Handmade somatic cell cloning in cattle / Gábor Vatja, Ian M. Lewis, and R. Tayfur Tecirlioglu -- Genetic modification of sheep by nuclear transfer with gene-targeted somatic cells / A. John Clark, Sarah Burl, and Chris Denning -- Transgenesis using nuclear transfer in goats / Anthoula Lazaris ... [et al.] -- Nuclear reprogramming : an overview / Takashi Tada -- Expression of imprinted genes in cloned mice / Takashi Kohda, Fumitoshi Ishino, and Atsuo Ogura -- Nuclear remodeling assay in xenopus egg extract / Koichi Gonda and Nobuaki Kikyo -- In vitro reprogramming of nuclei and cells / Anne-Mari Håkelien ... [et al.] -- Techniques for nuclear transfer to mouse embryonic stem cells / Danielè Pralong and Paul J. Verma -- Gene expression and methylation patterns in cloned embryos / Christine Wrenzycki ... [et al.] -- Are there any normal clones? / Ian Wilmut -- Future and applications of cloning / Alan O. Trounson. , Origin and progress of nuclear transfer in nonmammalian animals / Marie A. Di Berardino -- In vitro maturation and embryo production in cattle / Marc-André Sirard and Karine Coenen -- Activation of oocytes after nuclear transfer / Zoltán Macháty -- Culture medium preferences of pre-implantation cloned mouse embryos / Björn Heindryckx, Josiane Van der Elst, and Marc Dhont -- Maintenance of pregnancy in pigs with limited viable embryos / Tim King and Paul A. De Sousa -- Health and neonatal care of bovine clones / Jacqui T. Forsyth and David N. Wells -- Optimization of procedures for cloning by somatic cell nuclear transfer in mice / Young Gie Chung, Shaorong Gao, and Keith E. Latham -- Production of cloned calves using roscovitine-treated adult somatic cells as donors / Kazuchika Miyoshi, Sezen Arat, and Steven L. Stice -- A double nuclear transfer technique for cloning pigs / Irina A. Polejaeva, Shawn Walker, and Keith Campbell -- Nuclear transfer in non-human primates / Shoukhrat M. Mitalipov and Don P. Wolf --
    Language: English
    Subjects: Biology
    RVK:
    Keywords: Wirbeltiere ; Kerntransplantation ; Wirbeltiere ; Klonierung ; Kerntransplantation ; Kerntransplantation ; Klonierung ; Gentransfer ; Transgene Tiere ; Aufsatzsammlung
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