Molecular Imaging and Biology, 2017, Vol.19(2), pp.203-214
To access, purchase, authenticate, or subscribe to the full-text of this article, please visit this link: http://dx.doi.org/10.1007/s11307-016-1006-1 Byline: Giselle A. Suero-Abreu (1,2,3), Orlando Aristizabal (1), Benjamin B. Bartelle (1,4), Eugenia Volkova (1), Joe J. Rodriguez (1), Daniel H. Turnbull (1,2,3,5) Keywords: Ts-Biotag; Tie2 expression; B16 Melanoma; Ultrasound; MRI; Near infrared Abstract: Purpose In this study, we evaluated a genetic approach for in vivo multimodal molecular imaging of vasculature in a mouse model of melanoma. Procedures We used a novel transgenic mouse, Ts-Biotag, that genetically biotinylates vascular endothelial cells. After inoculating these mice with B16 melanoma cells, we selectively targeted endothelial cells with (strept)avidinated contrast agents to achieve multimodal contrast enhancement of Tie2-expressing blood vessels during tumor progression. Results This genetic targeting system provided selective labeling of tumor vasculature and showed in vivo binding of avidinated probes with high specificity and sensitivity using microscopy, near infrared, ultrasound, and magnetic resonance imaging. We further demonstrated the feasibility of conducting longitudinal three-dimensional (3D) targeted imaging studies to dynamically assess changes in vascular Tie2 from early to advanced tumor stages. Conclusions Our results validated the Ts-Biotag mouse as a multimodal targeted imaging system with the potential to provide spatio-temporal information about dynamic changes in vasculature during tumor progression. Author Affiliation: (1) Skirball Institute of Biomolecular Medicine, New York University School of Medicine (NYUSoM), 540 First Ave, New York, NY, 10016, USA (2) Biomedical Imaging Graduate Program, NYUSoM, New York, NY, USA (3) Department of Radiology, NYUSoM, New York, NY, USA (4) Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA (5) Department of Pathology, NYUSoM, New York, NY, USA Article History: Registration Date: 07/09/2016 Online Date: 27/09/2016 Article note: Electronic supplementary material The online version of this article (doi: 10.1007/s11307-016-1006-1) contains supplementary material, which is available to authorized users.
- ; Tie2 expression ; B16 Melanoma ; Ultrasound ; MRI ; Near infrared
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