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  • 1
    Language: English
    In: Scientia Medica, 2010, Vol.20(1), p.
    Description: AIMS: The purpose of this study was to compare the performance of three automated immunoassays for the detection of IgM and IgG Toxoplasma gondii antibodies using sera of pregnant women living in Colombia, a Latin American country with a high seroprevalence. METHODS: A total of 905 sera were tested for IgM antibodies and 914 for IgG antibodies with AxSYM, VIDAS and VIDIA immunoassays. Discrepancies were resolved by using the dye test for IgG antibodies, and the ISAGA test for IgM. RESULTS: The overall agreement between AxSYM, VIDAS and VIDIA assays was excellent for detection of IgG and IgM antibodies, and discrepancies were relatively rare (3.6% and 5.5% of sera for IgG and IgM antibodies, respectively). The performance of the three immunoassays was similar for the detection of IgG antibodies with high sensitivity (100.00% for VIDIA, 99.59% for VIDAS, 99.38% for AxSYM) and specificity (99.04% for VIDIA, 98.82% for AxSYM, 98.57% for VIDAS). The specificity for IgM antibodies was excellent for the three immunassays (99.88% for VIDIA, 99.76% for AxSYM and VIDAS). The sensitivity of the detection of IgM antibodies was higher with VIDIA (95.12%) than with VIDAS (76.74%) and AxSYM (61.90%) assays. The correlation between IgG titers was limited between AxSYM and VIDAS assays and between AxSYM and VIDIA assays, but was excellent between VIDIA and VIDAS assays. CONCLUSIONS: Our study performed with Latin American sera confirmed the excellent specificity of AxSYM, VIDAS and VIDIA assays for the detection of IgG and IgM antibodies already reported in other countries. The sensitivity of the detection of IgG antibodies was slightly higher with VIDIA than with VIDAS and AxSYM assays. The sensitivity of the detection of IgM antibodies was higher with VIDIA than with VIDAS and AxSYM assays.
    Keywords: Serologic Tests ; Enzyme - Linked Immunosorbent Assay ; Latin America ; Medicina ; Toxoplasmosis
    ISSN: 1980-6108
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  • 2
    In: British Journal of Nutrition, 2017, Vol.117(11), pp.1560-1569
    Description: Abstract Dietary carotenoid intake, especially from fruits and vegetables, has been associated with a reduced incidence of several chronic diseases. However, its bioavailability can vary, depending on the food matrix and host factors. Recently, it has been suggested that divalent minerals negatively impinge on carotenoid bioavailability by reducing bile-salt and non-esterified fatty-acid levels in the gut, which normally aid in emulsifying carotenoids. The aim of the present study was to investigate whether supplemental Ca would negatively influence carotenoid absorption in humans. A total of twenty-five healthy, non-obese men (age: 20–46 years, BMI〈30 kg/m 2 ) were recruited for this postprandial, randomised, crossover, double-blinded trial. Following a randomised block design, each participant received (after 2-week washout periods), on three occasions separated by 1 week, 270 g of spinach-based meals (8·61 ( sd 1·08) mg carotenoids/100 g fresh weight), supplemented with 0, 500 or 1000 mg of Ca (as calcium carbonate), with each participant acting as his or her own control. Blood samples were collected at regular postprandial intervals for up to 10 h following test meal intake, and standardised lunches were served. TAG-rich lipoprotein fractions were separated and carotenoid concentrations determined. AUC for meals without supplemented Ca were 22·72 ( sem 2·78) nmol×h/l (lutein), 0·19 ( sem 3·90) nmol×h/l ( β -carotene) and 2·80 ( sem 1·75) nmol×h/l ( β -cryptoxanthin). No significant influence of supplementation with either 500 or 1000 mg of supplemental Ca was found. In conclusion, Ca – the most abundant divalent mineral in the diet – given at high but physiological concentrations, does not appear to have repercussions on the bioavailability of carotenoids from a spinach-based meal.
    Keywords: Full Papers; Human And Clinical Nutrition; Calcium; Carotenoids; Bile-Salt Complexation; Soap Formation; Digestion
    ISSN: 0007-1145
    E-ISSN: 1475-2662
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  • 3
    Language: English
    In: Expert Review of Molecular Diagnostics, 01 May 2006, Vol.6(3), pp.399-411
    Description: Due to technical improvements and new developments of immunological assays, the reliability of serological laboratory diagnosis of HIV infection has improved considerably and the residual risk, due to the diagnostic window for transfusion-transmitted HIV, has been reduced significantly. Through...
    Keywords: Diagnostic Window ; HIV-1 RNA ; HIV Antigen ; Nucleic Acid Amplification Test ; Primary HIV Infection ; Medicine
    ISSN: 1473-7159
    E-ISSN: 1744-8352
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  • 4
    In: AIDS, 2003, Vol.17(6), pp.931-933
    Keywords: AIDS Serodiagnosis -- Methods ; HIV Seropositivity -- Diagnosis;
    ISSN: 0269-9370
    E-ISSN: 14735571
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  • 5
    Language: English
    In: Journal of clinical microbiology, November 2002, Vol.40(11), pp.4402-3; author reply 4403-4
    Description: Recently, 4th generation assays that permit the combined detection of human immunodeficiency virus type 1 (HIV-1) p24 antigen (Ag) and anti-HIV antibody (Ab) were introduced on the international market. The diagnostic window is reduced on average by 4 days with this new assay generation in comparison to 3rd generation antibody-screening enzyme immunoassays (EIAs) in HIV-1 group M subtype B primary infections (1, 6). Up to now, no data had been available on the sensitivity of this new generation of assays for detection of HIV-1 non-B subtype primary infection.
    Keywords: AIDS Serodiagnosis ; HIV-1 ; HIV-2 ; HIV Antibodies -- Blood ; HIV Antigens -- Blood ; HIV Seropositivity -- Diagnosis
    ISSN: 0095-1137
    E-ISSN: 1098660X
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  • 6
    In: Journal of Clinical Microbiology, 1998, Vol. 36(2), p.580
    Description: An international multicenter study was performed to evaluate a new, automated human immunodeficiency virus (HIV) third-generation antibody assay. The Enzymun-Test Anti-HIV 1 + 2 + Subtyp O showed 100% sensitivity and 99.8% specificity among 11,172 samples from hospitalized patients and blood donors. For early HIV antibody detection in seroconversion panels, Enzymun-Test showed a sensitivity equivalent to that of the Abbott Recomb. HIV-1/HIV-2 3rd Gen. assay.
    Keywords: Medicine ; Biology;
    ISSN: 0095-1137
    ISSN: 00951137
    E-ISSN: 1098660X
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  • 7
    Language: English
    In: Medical & Biological Engineering & Computing, 2010, Vol.48(8), pp.833-835
    Description: This study of intra-subject random variations of stabilometric parameters was achieved in the context of the standardized clinical stabilometry, in use in France and Southern Europe since 1985. The outstanding interest of stabilometry to follow up patients makes the results of this study indispensable for clinicians and their international publication is particularly important as, these days, the standardization Committee for clinical stabilometry resumes its work within the International Society for Postural and Gait Research. Such a study is possible only on the topological stabilometric parameters because of the stroboscopic effect on the dynamic parameters of the sampling rate of our computerized measuring chains.
    Keywords: Stabilometry ; Follow up ; Repeatability
    ISSN: 0140-0118
    E-ISSN: 1741-0444
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  • 8
    Language: English
    In: Industrial and Engineering Chemistry Research, 22 February 2016, Vol.55(12)
    Description: Integrated sustainability metrics provide an enriched set of information to inform decision making. However, such approaches are rarely used to assess product supply chains. In this work, four integrated metrics-presented in terms of land, resources, value added, and stability-are applied in a Me cycle context, along with industrial process systems assessment and life cycle assessment, to evaluate Bounty paper towels from two manufacturing lines. The results show that the paper towels from the more state-of the art manufacturing line and newer facility are marginally more sustainable by the majority of environmental measures. Drivers of impacts from land use, resource use, and externality costs in the product life cycle are largely in the supply chains for raw materials and energy. The integrated metrics point to greenhouse gases, criteria air pollutants, land used for pulpwood, and fossil fuel use as important emissions and resources to manage for improving the sustainability of paper towel production. The metrics employed help to reconcile contrasting results from the life cycle impact assessment, such as water and energy use impacts, and provide a reduced set of practical, yet comprehensive, information to inform product related decision making.
    Keywords: Engineering
    ISSN: 0888-5885
    E-ISSN: 1520-5045
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  • 9
    Language: French
    In: Kinésithérapie, la revue, June 2010, Vol.10(102), pp.25-25
    Keywords: Stabilisation Du Regard ; Temps D’appui Monopodal ; Dyslexiques ; Trouble Cognitif ; Stabilité Posturale ; Controlling Eye Contact ; Single Leg Stance Time ; Dyslexia ; Cognitive Disorder ; Postural Stability ; Physical Therapy
    ISSN: 1779-0123
    E-ISSN: 2213-1566
    Source: ScienceDirect Journals (Elsevier)
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  • 10
    Language: English
    In: Expert Review of Molecular Diagnostics, 01 January 2005, Vol.5(1), pp.75-91
    Description: Recent developments in the laboratory diagnosis of hepatitis B virus infection include the optimization of key serologic markers, including hepatitis B virus surface antigen and antihepatitis B virus core antibody, as well as the development of automated nucleic acid amplification assays. There...
    Keywords: Antihbc ; Antihbc-Igm ; Blood Donor Screening ; Diagnostic Window ; Hbsag ; Nat ; Surface Antigen Mutant ; Viral Load ; Medicine
    ISSN: 1473-7159
    E-ISSN: 1744-8352
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