Kooperativer Bibliotheksverbund

Berlin Brandenburg

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  • 1
    Language: English
    In: Journal of agricultural and food chemistry, 29 July 2015, Vol.63(29), pp.6651-2
    ISSN: 00218561
    E-ISSN: 1520-5118
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  • 2
    Language: English
    In: Proceedings of the National Academy of Sciences of the United States of America, 05 March 2013, Vol.110(10), pp.3782-7
    Description: The study of genetic disease mechanisms relies mostly on targeted mouse mutants that are derived from engineered embryonic stem (ES) cells. Nevertheless, the establishment of mutant ES cells is laborious and time-consuming, restricting the study of the increasing number of human disease mutations discovered by high-throughput genomic analysis. Here, we present an advanced approach for the production of mouse disease models by microinjection of transcription activator-like effector nucleases (TALENs) and synthetic oligodeoxynucleotides into one-cell embryos. Within 2 d of embryo injection, we created and corrected chocolate missense mutations in the small GTPase RAB38; a regulator of intracellular vesicle trafficking and phenotypic model of Hermansky-Pudlak syndrome. Because ES cell cultures and targeting vectors are not required, this technology enables instant germline modifications, making heterozygous mutants available within 18 wk. The key features of direct mutagenesis by TALENs and oligodeoxynucleotides, minimal effort and high speed, catalyze the generation of future in vivo models for the study of human disease mechanisms and interventions.
    Keywords: Disease Models, Animal ; Germ-Line Mutation ; Genetic Diseases, Inborn -- Genetics ; Oligodeoxyribonucleotides -- Administration & Dosage
    ISSN: 00278424
    E-ISSN: 1091-6490
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  • 3
    Language: English
    In: Phytochemistry, 2015, Vol.116, p.320(9)
    Description: To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.phytochem.2015.04.009 Byline: Daniel Wefers, Bianca M. Gmeiner, Catrin E. Tyl, Mirko Bunzel Abstract: Two diferuloylated arabinan oligosaccharides were isolated from quinoa seeds and characterized by NMR spectroscopy. The structural characteristics of the diferuloylated polysaccharides were characterized. Display Omitted Article History: Received 12 December 2014; Revised 24 April 2015
    Keywords: Quinoa ; Polysaccharides ; Nuclear Magnetic Resonance Spectroscopy
    ISSN: 0031-9422
    Source: Cengage Learning, Inc.
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  • 4
    Language: English
    In: Journal of agricultural and food chemistry, 21 December 2016, Vol.64(50), pp.9559-9568
    Description: Pectic arabinans and galactans presumably affect the physiological and technological properties of plant cell walls and dietary fiber. Their complex structures are usually analyzed by time-consuming methods, which are based on chemical cleavage to monomers. To gain more detailed insights into the arabinan and galactan structures, a time-efficient approach based on enzymatic cleavage and two-dimensional NMR spectroscopy was developed. Heteronuclear single quantum coherence spectroscopy (HSQC) marker signals were evaluated for various structural elements, and relative response factors were determined, allowing a semiquantitative estimation of the structural composition. The method was applied to analyze different insoluble plant materials and soluble polysaccharides. It was demonstrated that the developed approach yielded comparable information about various structural elements that can also be detected by using the conventional methylation analysis. However, by using the NMR method, additional structural information, such as the anomeric configuration of the monomers, is obtained, demonstrating the value of this novel approach.
    Keywords: NMR Spectroscopy ; Dietary Fiber ; Pectins ; Polysaccharides ; Screening ; Magnetic Resonance Spectroscopy ; Dietary Fiber -- Analysis ; Galactans -- Chemistry ; Polysaccharides -- Chemistry
    ISSN: 00218561
    E-ISSN: 1520-5118
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  • 5
    Language: English
    In: Journal of agricultural and food chemistry, 08 June 2016, Vol.64(22), pp.4656-64
    Description: Arabinans and galactans are complex pectic polysaccharides, which greatly influence the physicochemical and physiological properties of plants and plant-based foods. Conventional methods to characterize these challenging polymers are based on derivatization and/or unselective chemical cleavage of the glycosidic bonds of the polysaccharides, resulting in partial loss of essential information such as anomeric configuration. Here, endo-arabinanase and endo-galactanase were used to selectively cleave pectic arabinans and galactans. The liberated oligosaccharides were purified and characterized by LC-MS and one- and two-dimensional NMR spectroscopy resulting in known but also several previously unknown pectic structural elements. For the routine analysis of pectin hydrolysates by HPAEC-PAD, incubation conditions, chromatographic parameters, and relative response factors of the isolated pectic oligosaccharides against an internal standard were determined. The applicability of the method was demonstrated by analyzing different well-characterized plant cell wall materials. It was demonstrated that the developed method yields additional information about pectic arabinan and galactan structures that is not obtained from conventional methods such as methylation analysis.
    Keywords: Hpaec-Pad ; Dietary Fiber ; Oligosaccharide Profiling ; Pectic Arabinans and Galactans ; Pectins ; Plant Cell Wall Constituents ; Screening ; Selective Enzymatic Hydrolysis ; Chromatography, Ion Exchange -- Methods ; Galactans -- Chemistry ; Oligosaccharides -- Chemistry ; Plant Extracts -- Chemistry ; Polysaccharides -- Chemistry
    ISSN: 00218561
    E-ISSN: 1520-5118
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  • 6
    Article
    Article
    BMJ Publishing Group Ltd and British Association of Sport and Exercise Medicine
    Language: English
    In: British Journal of Sports Medicine, 31 August 2016
    Description: In their recent editorial, Batten et al1 discuss the high risk of injury during penalty corners in field hockey. The authors note the potential risk of head injuries caused by the frequently used drag flicks during penalty corners in field hockey and recommend the removal of the penalty corner and a change involving the use of a specialised power play. The authors consequently recommend further injury research on field hockey penalty corners.
    Keywords: Field Hockey ; Injuries
    ISSN: 0306-3674
    ISSN: 03063674
    E-ISSN: 1473-0480
    E-ISSN: 14730480
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  • 7
    Language: English
    In: Phytochemistry, August 2015, Vol.116, pp.320-328
    Description: Two diferuloylated arabinan oligosaccharides were isolated from quinoa seeds and characterized by NMR spectroscopy. The structural characteristics of the diferuloylated polysaccharides were characterized. In plants belonging to the order of Caryophyllales, pectic neutral side chains can be substituted with ferulic acid. The ability of ferulic acid to form intra- and/or intermolecular polysaccharide cross-links by dimerization was shown by the isolation and characterization of diferulic acid oligosaccharides from monocotyledonous plants. In this study, two diferulic acid oligosaccharides were isolated from the enzymatic hydrolyzate of seeds of the dicotyledonous pseudocereal quinoa by gel permeation chromatography and preparative HPLC and unambiguously identified by LC–MS and 1D/2D NMR spectroscopy. The isolated oligosaccharides are comprised of 5-5- and 8- -4-diferulic acid linked to the 2-position of the nonreducing residue of two (1 → 5)-linked arabinobioses. To get insight into the structure and the degree of phenolic acid substitution of the diferuloylated polysaccharides, polymeric sugar composition, glycosidic linkages, and polysaccharide-bound monomeric phenolic acids and diferulic acids were analyzed. This study demonstrates that diferulic acids are involved into intramolecular and/or intermolecular cross-linking of arabinan chains and may have a major impact on cell wall architecture of quinoa and other dicotyledonous plants of the order of Caryophyllales.
    Keywords: Chenopodium Quinoa ; Amaranthaceae ; Cell Wall ; Polysaccharides ; Pectins ; Dietary Fiber ; Arabinans ; Diferulic Acids ; Oligosaccharides ; NMR ; Botany
    ISSN: 0031-9422
    E-ISSN: 1873-3700
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  • 8
    Language: English
    In: Journal of agricultural and food chemistry, 21 January 2015, Vol.63(2), pp.707-15
    Description: Amaranth is a pseudocereal of high nutritional value, including a high dietary fiber content. Amaranth dietary fiber was suggested to contain large amounts of neutral rhamnogalacturonan I side chains. In this study, endo-arabinanase and endo-galactanase were used to liberate arabinan and galactan oligosaccharides from amaranth fiber. The liberated oligosaccharides were identified by high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and HPLC-MS(n) using standard compounds, which were isolated from amaranth, sugar beet, potato, and red clover sprouts and characterized by one- and two-dimensional NMR spectroscopy. It was demonstrated that insoluble amaranth arabinans have linear and branched areas, with the O-3 position being the dominant branching point. Minor amounts of branches at position O-2 and double substitution were also found. Amaranth arabinans were also demonstrated to contain terminal α-(1→5)-linked l-arabinopyranose units. In addition, it was evidenced that galactans from amaranth seeds are composed of β-(1→4)-linked d-galactopyranose units, which can also be terminated with l-arabinopyranose units. In direct comparison to structural elucidation of amaranth fiber by using methylation analysis, the advantage of the enzymatic approach over methylation analysis was demonstrated.
    Keywords: Amaranthus Hypochrondriacus ; NMR ; Arabinans ; Arabinopyranose ; Cell Wall ; Dietary Fiber ; Galactans ; Methylation Analysis ; Oligosaccharides ; Amaranthus -- Chemistry ; Galactans -- Chemistry ; Pectins -- Chemistry ; Plant Extracts -- Chemistry ; Polysaccharides -- Chemistry
    ISSN: 00218561
    E-ISSN: 1520-5118
    E-ISSN: 22962646
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  • 9
    Language: English
    In: Genetics, November 2013, Vol.195(3), pp.703-13
    Description: Targeted mouse mutants are instrumental for the analysis of gene function in health and disease. We recently provided proof-of-principle for the fast-track mutagenesis of the mouse genome, using transcription activator-like effector nucleases (TALENs) in one-cell embryos. Here we report a routine procedure for the efficient production of disease-related knockin and knockout mutants, using improved TALEN mRNAs that include a plasmid-coded poly(A) tail (TALEN-95A), circumventing the problematic in vitro polyadenylation step. To knock out the C9orf72 gene as a model of frontotemporal lobar degeneration, TALEN-95A mutagenesis induced sequence deletions in 41% of pups derived from microinjected embryos. Using TALENs together with mutagenic oligodeoxynucleotides, we introduced amyotrophic lateral sclerosis patient-derived missense mutations in the fused in sarcoma (Fus) gene at a rate of 6.8%. For the simple identification of TALEN-induced mutants and their progeny we validate high-resolution melt analysis (HRMA) of PCR products as a sensitive and universal genotyping tool. Furthermore, HRMA of off-target sites in mutant founder mice revealed no evidence for undesired TALEN-mediated processing of related genomic sequences. The combination of TALEN-95A mRNAs for enhanced mutagenesis and of HRMA for simplified genotyping enables the accelerated, routine production of new mouse models for the study of genetic disease mechanisms.
    Keywords: C9orf72 ; Fus ; Talens ; Disease Model ; Mouse Mutant ; Nuclease ; One-Cell Embryo ; Mutagenesis ; Genetic Engineering -- Methods
    ISSN: 00166731
    E-ISSN: 1943-2631
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  • 10
    Language: English
    In: Zeitschrift für Bibliothekswesen und Bibliographie, 02/15/2017, Vol.64(1), pp.021-025
    ISSN: 00442380
    E-ISSN: 18642950
    Source: CrossRef
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