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  • 1
    Language: English
    In: team.konkret, 2016, Vol.12(03), pp.4-7
    ISSN: 1869-3202
    E-ISSN: 1869-3210
    Source: Thieme Publishing Group
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  • 2
    In: team.konkret, 2017, Vol.13(02)
    In: team.konkret, 2017, Vol.13(02), pp.4-7
    ISSN: 1869-3202
    E-ISSN: 1869-3210
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  • 3
    Article
    Article
    Language: German
    In: team.konkret, 6/2012, Vol.8(02), pp.38-42
    ISSN: 1869-3202
    E-ISSN: 1869-3210
    Source: Thieme Publishing Group (via CrossRef)
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  • 4
    Language: German
    In: Tierärztliche Praxis Ausgabe G: Großtiere / Nutztiere, 2013, Vol.41(03), pp.145-145
    ISSN: 1434-1220
    E-ISSN: 2567-5834
    Source: Thieme Publishing Group (via CrossRef)
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  • 5
    Language: English
    In: The Journal of biological chemistry, 28 June 2013, Vol.288(26), pp.18825-33
    Description: Fertilization in animals is a complex sequence of several biochemical events beginning with the insemination into the female reproductive tract and, finally, leading to embryogenesis. Studies by Kitajima and co-workers (Miyata, S., Sato, C., and Kitajima, K. (2007) Trends Glycosci. Glyc, 19, 85-98) demonstrated the presence of polysialic acid (polySia) on sea urchin sperm. Based on these results, we became interested in the potential involvement of sialic acid polymers in mammalian fertilization. Therefore, we isolated human sperm and performed analyses, including Western blotting and mild 1,2-diamino-4,5-methylenedioxybenzene-HPLC, that revealed the presence α2,8-linked polySia chains. Further analysis by a glyco-proteomics approach led to the identification of two polySia carriers. Interestingly, besides the neural cell adhesion molecule, the polysialyltransferase ST8SiaII has also been found to be a target for polysialylation. Further analysis of testis and epididymis tissue sections demonstrated that only epithelial cells of the caput were polySia-positive. During the epididymal transit, polySia carriers were partially integrated into the sperm membrane of the postacrosomal region. Because polySia is known to counteract histone as well as neutrophil extracellular trap-mediated cytotoxicity against host cells, which plays a role after insemination, we propose that polySia in semen represents a cytoprotective element to increase the number of vital sperm.
    Keywords: Cell Adhesion ; Fertilization ; Mammalian Sperm ; Ncam ; Polysaccharide ; Polysialic Acid ; Polysialyltransferase ; Post-Translational Modification ; St8siaii ; Spermatozoa ; Protein Processing, Post-Translational ; Neural Cell Adhesion Molecules -- Metabolism ; Semen -- Metabolism ; Sialic Acids -- Metabolism ; Sialyltransferases -- Metabolism
    E-ISSN: 1083-351X
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  • 6
    Language: English
    In: Journal of Dairy Science, October 2017, Vol.100(10), pp.8287-8295
    Description: The objective of the study was to assess the value of quantitative multiplex real-time PCR examination of bulk tank milk samples for bovine mastitis pathogens as a tool for herd level diagnosis. Using a logistic regression model, this study is aimed at calculating the threshold level of the apparent within-herd prevalence as determined by quarter milk sample cultivation of all lactating cows, thus allowing the detection of a herd positive for a specific pathogen within certain probability levels. A total of 6,335 quarter milk samples were collected and cultured from 1,615 cows on 51 farms in Germany. Bulk tank milk samples were taken from each farm and tested by bacterial culture as well as the commercial PCR assay Mastit 4A (DNA Diagnostic A/S, Risskov, Denmark) identifying , , , and . In addition, PCR was performed on pooled herd milk samples containing milk aliquots from all lactating cows in each of the 51 herds. Only 1 out of the 51 herds was found PCR positive for in bulk tank and pooled herd milk samples, and cultured quarter milk samples. Spearman's rank correlations between the cycle threshold value of bulk tank milk PCR and the apparent within-herd prevalence were calculated in regard to , , and . For these pathogens, significant correlations were found. If 1 bulk tank milk sample per herd was tested, the estimated within-herd prevalence thresholds for 90% probability of detection were 27.6% for , 9.2% for , and 13.8% for on the cow level. On the quarter level, the within-herd prevalence had to be at least 32.6% for s, 1.7% for , and 4.3% for to detect a herd as positive using a single bulk milk sample. The results indicate that mastitis pathogens in bulk tank milk can be identified by the applied PCR assay. Bulk tank milk examination is not a reliable tool for the identification of the named pathogens by single testing, but might be a valuable monitoring tool when used frequently with repeated testing. Furthermore, this approach could be a useful monitoring tool for detecting new pathogen occurrence in the herd.
    Keywords: Staphylococcus Aureus ; Streptococcus ; Herd Level Diagnosis ; Pooled Milk Sample ; Agriculture
    ISSN: 0022-0302
    E-ISSN: 1525-3198
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  • 7
    Language: German
    In: Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere, 2013, Vol.41(01), pp.05-06
    ISSN: 1434-1239
    E-ISSN: 2567-5842
    Source: Thieme Publishing Group (via CrossRef)
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  • 8
    Language: German
    In: Tierarztliche Praxis. Ausgabe K, Kleintiere/Heimtiere, 20 June 2017, Vol.45(3), pp.199-203
    Description: Ultrasound evaluation of the ovaries is an important element of the gynaecological examination. Although it is a well-established method used for many domestic animals, it is an uncommon procedure in cats. This article describes the technical requirements, the examination procedure, and the sonographic appearance of the physiological ovary in the cat. The examination can be performed with the cat in lateral position or dorsal recumbency. Usually the ovaries are found caudal to the kidneys which serve as lead structure. The median size of an ovary in anestrous is 0.9 cm (length) x 0.5 cm (height) x 0.4 cm (width). The ovaries of cyclical (follicular and luteal phase) cats are easier to detect than ovaries of cats in interestrous or anestrous.
    Keywords: Ultrasound ; Cat ; Gynaecology ; Ovary ; Cats -- Anatomy & Histology ; Ovary -- Diagnostic Imaging ; Ultrasonography -- Veterinary
    ISSN: 14341239
    E-ISSN: 2567-5842
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  • 9
    Language: German
    In: Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere, 2013, Vol.41(01), pp.47-52
    ISSN: 1434-1239
    E-ISSN: 2567-5842
    Source: Thieme Publishing Group (via CrossRef)
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  • 10
    Language: German
    In: Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere, 2012, Vol.40(01), pp.5-6
    ISSN: 1434-1239
    E-ISSN: 2567-5842
    Source: Thieme Publishing Group (via CrossRef)
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