Format:
1 Online-Ressource (13 Seiten)
Content:
The genome of influenza A viruses (IAV) is encoded in eight distinct viral ribonucleoproteins
(vRNPs) that consist of negative sense viral RNA (vRNA) covered by the IAV nucleoprotein.
Previous studies strongly support a selective packaging model by which vRNP segments are
bundling to an octameric complex, which is integrated into budding virions. However, the
pathway(s) generating a complete genome bundle is not known. We here use a multiplexed
FISH assay to monitor all eight vRNAs in parallel in human lung epithelial cells. Analysis of
3.9 × 105 spots of colocalizing vRNAs provides quantitative insights into segment composition of vRNP complexes and, thus, implications for bundling routes. The complexes rarely contain multiple copies of a specific segment. The data suggest a selective packaging
mechanism with limited flexibility by which vRNPs assemble into a complete IAV genome.
We surmise that this flexibility forms an essential basis for the development of reassortant
viruses with pandemic potential.
Content:
Peer Reviewed
Note:
This article was supported by the German Research Foundation (DFG) and the Open Access Publication Fund of Humboldt-Universität zu Berlin.
In:
London : Nature Publishing Group UK, 11
Language:
English
DOI:
10.1038/s41467-020-18108-1
URN:
urn:nbn:de:kobv:11-110-18452/24725-2
URL:
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