Format:
1 Online-Ressource (xvii, 155, 14316 KB)
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Illustrationen, Diagramme
Content:
Chloroplasts are the photosynthetic organelles in plant and algae cells that enable photoautotrophic growth. Due to their prokaryotic origin, modern-day chloroplast genomes harbor 100 to 200 genes. These genes encode for core components of the photosynthetic complexes and the chloroplast gene expression machinery, making most of them essential for the viability of the organism. The regulation of those genes is predominated by translational adjustments. The powerful technique of ribosome profiling was successfully used to generate highly resolved pictures of the translational landscape of Arabidopsis thaliana cytosol, identifying translation of upstream open reading frames and long non-coding transcripts. In addition, differences in plastidial translation and ribosomal pausing sites were addressed with this method. However, a highly resolved picture of the chloroplast translatome is missing. Here, with the use of chloroplast isolation and targeted ribosome affinity purification, I generated highly enriched ribosome profiling datasets of the chloroplasts translatome for Nicotiana tabacum in the dark and light. [...]
Note:
Dissertation Universität Potsdam 2021
Additional Edition:
Erscheint auch als Druck-Ausgabe Schuster, Maja High resolution decoding of the tobacco chloroplast translatome and its dynamics during light-intensity acclimation Potsdam, 2021
Language:
English
Keywords:
Photosynthese
;
Chloroplast
;
Hochschulschrift
DOI:
10.25932/publishup-51268
URN:
urn:nbn:de:kobv:517-opus4-512680
URL:
https://doi.org/10.25932/publishup-51268
URL:
https://nbn-resolving.org/urn:nbn:de:kobv:517-opus4-512680
URL:
https://d-nb.info/1237319994/34
Author information:
Kühn, Kristina 1974-
Author information:
Bölter, Bettina 1972-
Author information:
Zoschke, Reimo 1979-
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