Kooperativer Bibliotheksverbund

Berlin Brandenburg

and
and

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Language: English
    In: Analytical and quantitative cytology and histology, 2007, Vol.29(1), pp.32-40
    Description: The incidence of early prostate cancer (PCa) among middle-aged men has increased rapidly. For many of these men, curatively intended treatment does more harm than good. Established prognostic factors are tumor stage and grade. As a result of earlier detection a majority of patients now have nonpalpable tumors (T1c) of intermediate grade (Gleason score 6). Prostate specific antigen in serum in such cases is generally at a low level and not a reliable predictor of prognosis. Altogether there is an urgent need for adjunctive prognostic indicators. In the search for relevant tumor markers for improved patient selection an exploration of the proteome (the human proteins) could be fruitful. This paper critically reviews the use of 2-dimensional gel electrophoresis (2-DE) for proteome research. Additional steps such as image analysis and mass spectrometry are described. Techniques based on non-2-DE platforms: surface-enhanced laser desorption/ionization (SELDI), isotope coded affinity tags (ICAT) and array-based technologies are also summarized. Although labor-intensive and time-consuming, 2-DE is presently the most powerful method for analysis of cellular protein phenotype and may potentially reveal gene regulations that cannot be detected on a genetic level.
    Keywords: Proteomics ; Prostatic Neoplasms -- Diagnosis;
    ISSN: 0884-6812
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 2
    Language: English
    In: Analytical and quantitative cytology and histology, 2005, Vol.27(5), pp.263-272
    Description: To investigate protein expression in prostate cancer using 2-dimensional gel electrophoresis (2-DE) and mass spectrometry. Cells were collected from 29 peripheral zone tumors and from benign tissue by scraping cut surfaces of radical prostatectomy specimens. Samples were suspended in a medium with protease inhibitors and prepared for 2-DE. Gels were analyzed, and protein spots that differed quantitatively between tumor and benign tissue were identified via mass spectrometric fingerprinting of tryptic fragments and tandem mass spectrometry sequence analysis. In total, 63 spots differed between cancer and benign samples (p 1.5 fold) in cancer and 7 underexpressed (〈 0.6 fold). Among overexpressed proteins were transcription factors (nucleoside diphosphate kinase 1) and enzymes involved in gene silencing (chromobox protein), protein synthesis (39S ribosomal protein L12, BiP protein, protein disulfide isomerase), degradation (cytosol aminopeptidase, endopeptidase Clp, inorganic pyrophosphatase) and energy metabolism (acyl-CoA dehydrogenase, isocitrate dehydrogenase, NADH-ubiquinone oxidoreductase, pyruvate dehydrogenase), heat-shock proteins (60 and 70 kd), structural proteins (cytokeratins) and membrane proteins (stomatinlike protein 2). The protein profile of prostate cancer differs from that of benign tissue. Several potential target proteins for detection or evaluation of prognosis in prostate cancer were identified.
    Keywords: Gene Expression Regulation, Neoplastic ; Proteomics ; Prostatic Neoplasms -- Metabolism;
    ISSN: 0884-6812
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 3
    Language: English
    In: MOLECULAR & CELLULAR PROTEOMICS, Vol.4, pp.S117-S117
    ISSN: 1535-9476
    Source: SwePub (National Library of Sweden)
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 4
    Language: English
    In: Proteomics, 2006, Vol.6(13), pp.3918-3925
    Description: For laboratory techniques that require well-preserved proteins, such as 2-DE, fresh tissue must be harvested and processed as fast as possible to avoid proteolytic degradation. We describe a modified method for harvesting tissue from radical prostatectomy...
    Keywords: Medical And Health Sciences ; Medicin Och Hälsovetenskap ; Medicine ; Medicin
    ISSN: 1615-9853
    E-ISSN: 16159861
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 5
    Language: English
    In: Proteomics, 2006, Vol.6(15), pp.4370-4380
    Description: The prognosis of prostate cancer correlates with tumor differentiation. Gleason score and DNA ploidy are two prognostic factors that correlate with prognosis. We analyzed differences in protein expression in prostate cancer of high and low aggressiveness according to these measures. From 35 prostatectomy...
    Keywords: Medical And Health Sciences ; Medicin Och Hälsovetenskap ; Medicine ; Medicin ; 2-De ; Dna Ploidy ; Gleason Score ; Prognosis ; Prostate Cancer
    ISSN: 1615-9853
    E-ISSN: 16159861
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 6
    Language: English
    In: Proteomics, 2005, Vol.5(10), pp.2570-2576
    Description: The prostate has three anatomical zones: the peripheral (PZ), the transition (TZ), and the central (CZ) zone. It is proposed that the CZ may be of mesodermal origin, whereas the other two are of endodermal origin. Proteome patterns in the zones were characterized to test for differences. Cells were scraped...
    Keywords: Prostate -- Chemistry ; Proteins -- Chemistry;
    ISSN: 1615-9853
    E-ISSN: 16159861
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 7
    Language: English
    In: MODERN PATHOLOGY, Vol.18, pp.152A-152A
    Description: Prader-Willi syndrome (PWS) is a multisystem genetic disorder characterized by short stature, muscular hypotonia, hyperphagia, obesity, maladaptive behaviour, hypogonadism and partial growth hormone (GH) deficiency (GHD). Severe GHD of other aetiologies has been shown to affect mood and quality of life negatively, and there are reports of improvements with GH replacement. We have studied cognitive, emotional, physical and social parameters in PWS adults at baseline, during and after GH treatment. Patients and methods Nineteen patients, 9 females and 10 males, median age 25 years, mean BMI 35 kgm2 participated in this study. Approximately half of the group had GHD. All patients fulfilled the clinical criteria for PWS and 13 had a positive genotype. The patients were randomized to 6 months of treatment with either GH 1.6 IU/day (0.53 mg/day) or placebo, followed by 12 months of active GH treatment. Treatment was then stopped, and the patients were followed for an additional period of 6 months. A test battery for general cognitive evaluation and a computer-based measurement of reaction time, motor speed and fluency were employed at baseline, after 6 months and at the end of GH treatment. At the same time intervals, a self-evaluation questionnaire was answered at the end of each test session. Other questionnaires reflecting the patients cognitive, emotional, physical and social status were answered by relatives caretakers at baseline and at 3 and 6 months following cessation of GH treatment. Baseline cognitive level was estimated to be moderately to mildly impaired; IQ range was 40-90. The results from some of the cognitive and the motor performance tests improved significantly after 6 and 18 months of GH treatment. According to the questionnaires, both the patients and the relatives caretakers evaluated physical status rather negatively at baseline, but still, impairments in both physical and social status and overall functioning were observed when GH treatment was discontinued. The self-evaluation did not change in any aspect during GH treatment. In this pilot study of an adult PWS cohort, we were able to document beneficial effects in mental speed and flexibility and in motor performance during GH treatment. Impairment was seen in physical and social status as well as overall functioning, when GH treatment stopped. Studies of larger cohorts are needed to further elucidate the role of GH treatment in this group of patients.
    Keywords: Patients ; Social Status ; Quality of Life ; Reaction Time ; Performance Tests ; Obesity ; Congenital Impairments ; Outcomes of Treatment ; Adults;
    ISSN: 0893-3952
    ISSN: 09642633
    E-ISSN: 18134424
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 8
    Language: English
    In: 16 December 1988, Vol.980, pp.75-81
    Description: Based on 20 years of experience in underwater imaging we try to present a generalized description of what the conditions for optical sensors in Swedish waters are like. An evaluation of different optical techniques for observation and inspection purposes is also performed.
    Keywords: Engineering
    ISBN: 9780819400154
    ISBN: 0819400157
    ISSN: 0277786X
    E-ISSN: 1996756X
    Source: SPIE Digital Library - Proceedings
    Source: SPIE Digital Library - Journals
    Source: SPIE Digital Library - eBooks
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 9
    Language: English
    In: 12 August 1988, Vol.925, pp.203-213
    Description: During the last years two new measurement systems are developed at FOA: the mobile profiling Hydro Optic Sensor System (ROSS) and the Anchored Daylight Attenuation Monitor (ADAM). The main objective of these efforts is to get reference data for the performance evaluation of laser systems. Both the systems measure daylight attenuation and HOSS measures transmission, back- and forward scattering as well. ADAM is placed within a test area together with a number of meteorological and hydrological sensors. All optical sensors are active in the blue-green (530-540 nm) part of the spectrum. In this part optical systems in the Baltic show optimum range performance.
    Keywords: Engineering
    ISBN: 9780892529605
    ISBN: 0892529601
    ISSN: 0277786X
    E-ISSN: 1996756X
    Source: SPIE Digital Library - Proceedings
    Source: SPIE Digital Library - Journals
    Source: SPIE Digital Library - eBooks
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. Further information can be found on the KOBV privacy pages