Format:
Online-Ressource
ISSN:
1469-3178
Content:
Abstract: Glioblastoma is a very aggressive tumor and represents the most common primary brain malignancy. Key characteristics include its high resistance against conventional treatments, such as radio‐ and chemotherapy and its diffuse tissue infiltration, preventing complete surgical resection. The analysis of migration and invasion processes in a physiological microenvironment allows for enhanced understanding of these phenomena and can lead to improved therapeutic approaches. Here, we combine two state‐of‐the‐art techniques, adult organotypic brain tissue slice culture (OTC) and light‐sheet fluorescence microscopy (LSFM) of cleared tissues in a combined method termed OTCxLSFM. Using this methodology, we can show that glioblastoma tissue infiltration can be effectively blocked through treatment with arsenic trioxide or WP1066, as well as genetic depletion of the tetraspanin, transmembrane receptor CD9, or signal transducer and activator of transcription 3 (STAT3). With our analysis pipeline, we gain single‐cell level, three‐dimensional information, as well as insights into the morphological appearance of the tumor cells.
In:
day:08
In:
month:11
In:
year:2023
In:
extent:24
In:
European Molecular Biology Organization, EMBO reports, Heidelberg : EMBO Press, [200]-, (08.11.2023) (gesamt 24), 1469-3178
Language:
English
DOI:
10.15252/embr.202356964
URN:
urn:nbn:de:101:1-2023110914012552616316
URL:
https://doi.org/10.15252/embr.202356964
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2023110914012552616316
URL:
https://d-nb.info/1309252939/34
URL:
https://doi.org/10.15252/embr.202356964
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