Format:
Online-Ressource
ISSN:
1522-2683
Content:
Abstract: A new fluorescent staining technique using o‐phthaldialdehyde (OPA) has been developed for the rapid post‐electrophoretic location for separated zones of proteins and glycoproteins. The method has also been applied to the detection of immunoprecipitates and, in the form of a spray reagent, to the detection of proteins separated on thin‐layer gel filtration plates. Major protein zones can be visualized within 1–2 min with maximum sensitivity being achieved within 15 min. In polyacrylamide and agarose gels, the sensitivity is of the same order as staining with Coomassie Blue G‐250. Reagents are very inexpensive and, because the reaction is performed at neutral or weekly alkaline pH in aqueous buffers, retention of enzymatic or other biological activity should be optimal. The method is thus particularly suitable for use in preparative applications and when it is desirable to combine general protein detection with specific location of zone of enzyme activity.
In:
volume:2
In:
number:2
In:
year:2005
In:
pages:76-81
In:
extent:6
In:
Electrophoresis, Weinheim : Wiley-Blackwell, 1980-, 2, Heft 2 (2005), 76-81 (gesamt 6), 1522-2683
Language:
English
DOI:
10.1002/elps.1150020203
URN:
urn:nbn:de:101:1-2024040207051681461049
URL:
https://doi.org/10.1002/elps.1150020203
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2024040207051681461049
URL:
https://d-nb.info/1324994002/34
URL:
https://doi.org/10.1002/elps.1150020203
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