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Berlin Brandenburg

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  • 1
    Language: English
    In: Gene, 1994, Vol.150(1), pp.17-25
    Description: El Tor biotype Vibrio cholerae strains express a cell-associated mannose-sensitive hemagglutinin (MSHA) which is a putative attachment factor. Several MSHA-negative mutants from V. cholerae strain JBK70 were previously generated by Tn5 mutagenesis [Finn et al., Infect. Immun. 55 (1987) 942–946]. The chromosomal DNA regions containing the Tn5 insertions were isolated from eight strains for further analysis. Nucleotide sequencing of the insertional junctions and corresponding clones containing the intact chromosomal region from the parental strain revealed the presence of several contiguous ORFs. Only two ORFs of this region had received insertions, and these showed remarkable homology to genes involved in the general secretory pathway found in several Gram − bacterial species. Proteins corresponding to the observed ORFs were visualized with the T7 promoter/RNA polymerase expression system. Marker exchange mutagenesis was used to insert kanamycin-resistance cassettes and Tn phoA insertions into different locations of this region in the chromosome of wild-type V. cholerae strains. The phenotypes of these mutants showed that this DNA region is involved in MSHA production, but is not required for general extracellular protein secretion.
    Keywords: Bacterial Adhesion ; Pili ; Toxin ; Transposon-Insertion Mutants ; Tnphoa ; T7 RNA Polymerase ; Protein Secretion ; Pullulanase ; Recombinant DNA ; Engineering ; Biology ; Anatomy & Physiology
    ISSN: 0378-1119
    E-ISSN: 1879-0038
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  • 2
    Language: English
    In: Group & Organization Management, September 1994, Vol.19(3), pp.295-308
    Description: An experimental design was employed to assess the impact of a managing diversity policy on participants' ratings of organizational attractiveness. Four hundred forty-eight upper level undergraduate management students were randomly assigned to either a g diversity or a control condition. Subjects were asked to read one of two forms of a recruitment brochure describing a fictitious company and then to rate the attractiveness of the company. As hypothesized, participants in the managing diversity condition rated the organization significantly more positively than did those in the control condition. There were also significant main effects for race and gender that accounted for more variance in the ratings of organizational attractiveness than did the managing diversity manipulation. Non-Whites and women had higher mean organizational attractiveness ratings than Whites and men, respectively. Managerial implications of the findings and future research directions are discussed.
    Keywords: Education ; Sociology & Social History ; Business ; Psychology
    ISSN: 1059-6011
    E-ISSN: 1552-3993
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  • 3
    Language: English
    In: Neuropsychologia, 1994, Vol.32(11), pp.1397-1408
    Description: Thirteen patients with left-hemisphere stroke and history of aphasia and 13 normal controls were administered the covert orientation of visual attention task (COVAT). This task presents targets to the right or left of a central fixation point after a cue (84% of trials) or with no cue (16% of trials). Left-hemisphere damaged patients also received tests of language function at the time of the study. For targets presented 100 msec after cue onset, normal controls demonstrated equivalent responding for targets to the left and to the right of a central fixation point. Patients with left-hemisphere damage showed slower reaction times when responding to targets on the right as opposed to the left side of space when attention was first cued to the opposite side of space (invalid trials) or when attention was focused on a central fixation point (uncued trials), but they did not show slower reaction times on the right side when attention was first cued to the right (valid trials). For left-sided targets, no differences between valid, invalid, and uncued trials existed. Slower responding to right- as opposed to left-sided targets on invalid and uncued trials was correlated with impaired performance on six of seven language measures for patients with left-hemisphere damage. Implications for the relationship between language and selective attention systems in the left hemisphere are discussed.
    Keywords: Attention ; Selective Attention ; Spatial Attention ; Visual Attention ; Aphasia and Language ; Medicine
    ISSN: 0028-3932
    E-ISSN: 1873-3514
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