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  • 1
    Language: English
    In: Microbial Pathogenesis, 1994, Vol.16(3), pp.243-247
    Description: Haemophilus ducreyi, Moraxella catarrhalis and a non-piliated Escherichia coli K-12 strain were studied for their ability to bind to human keratinocytes in vitro. Epidermal cells isolated from neonatal foreskins were grown to confluence in serum-free keratinocyte media. Probing of the monolayers with anti-cytokeratin antibody showed that 97% of cells were keratinocytes. Bacteria were grown to mid-log phase and seeded onto the monolayers. At various time-points monolayers were washed with PBS to remove non-adherent bacteria, and the monolayers were quantitatively cultured. After 120 min, 15 to 23% of the H. ducreyi inocula bound to the monolayer, while less than 1% of the M. catarrhalis or E. coli controls bound. Wet mounts of fixed monolayers observed with differential interference contrast microscopy confirmed the quantitative data. We conclude that H. ducreyi binds to keratinocytes and that this process may play a role in the initiation of chancroid.
    Keywords: Haemophilus Ducreyi; Adherence; Keratinocytes ; Biology ; Chemistry
    ISSN: 0882-4010
    E-ISSN: 1096-1208
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  • 2
    Language: English
    In: The Journal of Infectious Diseases, 1 May 1994, Vol.169(5), pp.1146-1150
    Description: Four subjects were experimentally infected with Haemophilus ducreyi. Lesions developed only at sites where live bacteria were inoculated on abraded skin. No subject developed fever, lymphadenopathy, or disseminated infection during a 3-day observation period. Two subjects who were rechallenged 2 months after initial infection also developed lesions. The amount of H. ducreyi recovered from 10 of 12 biopsies that were semiquantitatively cultured varied widely. Similar histologic features were present in initial and second infections. The epidermis contained pustules; the dermis contained an infiltrate of T cells and macrophages and reactive endothelial cells. Keratinocytes and T cells expressed HLA-DR, consistent with a delayed-type hypersensitivity response. The subjects did not mount humoral responses to bacterial proteins and to lipooligosaccharides after primary and secondary challenges. Thus, human experimental infection with H. ducreyi is well tolerated and safe. Recruitment of T cells and macrophages into chancroid lesions may partially explain the association between chancroid and human immunodeficiency virus transmission.
    Keywords: Biological sciences -- Biology -- Physiology -- Heterophils ; Biological sciences -- Biology -- Physiology -- Heterophils ; Health sciences -- Medical conditions -- Infections -- Heterophils ; Biological sciences -- Biology -- Physiology -- Heterophils ; Biological sciences -- Biochemistry -- Biomolecules -- Heterophils ; Biological sciences -- Biochemistry -- Biomolecules -- Heterophils ; Health sciences -- Medical conditions -- Diseases -- Heterophils ; Health sciences -- Medical conditions -- Infections -- Heterophils ; Biological sciences -- Biochemistry -- Biomolecules -- Heterophils ; Health sciences -- Medical conditions -- Diseases -- Heterophils
    ISSN: 00221899
    E-ISSN: 15376613
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