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  • 1
    Language: English
    In: Proceedings of the National Academy of Sciences of the United States of America, 24 August 2010, Vol.107(34), pp.15022-6
    Description: Gene targeting by homologous recombination in embryonic stem cells is extensively used to generate specific mouse mutants. However, most mammalian species lack tools for targeted gene manipulation. Since double-strand breaks strongly increase the rate of homologous recombination at genomic loci, we explored whether gene targeting can be directly performed in zygotes by the use of zinc-finger nucleases. Here we report that gene targeting is achieved in 1.7-4.5% of murine one-cell embryos upon the coinjection of targeting vectors with zinc-finger nucleases, without preselection. These findings enable the manipulation of the mammalian germ line in a single step in zygotes, independent of ES cells.
    Keywords: Endonucleases -- Metabolism ; Gene Targeting -- Methods ; Zygote -- Metabolism
    ISSN: 00278424
    E-ISSN: 1091-6490
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  • 2
    Language: English
    In: 8th International Conference On The Scientific And Clinical Applications Of Magnetic Carriers, Rostock, (Germany) (25–29 May 2010):
    In: AIP Conference Proceedings, 02 December 2010, Vol.1311(1), pp.431-437
    Description: The effect of magnetic nanoparticles on the survival of leukocytes in general and especially the lymphocytes as important parts of the immune system during incubation and separation was analyzed. Primary leukocytes were inoculated with magnetic core/carboxymethyl‐dextran (CMD) nanoparticles for 4 to 30 minutes. Labelled cells were separated by MACS, counted and analyzed by FACS. T‐Lymphocytes were identified by CD3 and B‐Lymphocytes by CD19. After magnetic separation granulocytes represented the majority of cells in both, the positive and negative fraction. Most of the lymphocytes were detected in the negative fraction. The number of T‐Lymphocytes in the positive fraction increased 2.5‐fold from 4 to 16 minutes, whereas the amount of B‐Lymphocytes remains constant. T cells could be expanded after short‐term incubation with nanoparticles indicating full biological activity. Our enrichment procedure of tumor cells from peripheral blood preserves the integrity and biological activity of leukocytes in the negative fraction.
    Keywords: Materials Physics and Applications
    ISBN: 978-0-7354-0866-1
    ISSN: 0094-243X
    E-ISSN: 1551-7616
    Source: © 2010 American Institute of Physics (AIP)〈img src=http://exlibris-pub.s3.amazonaws.com/AIP_edited.gif style="vertical-align:middle;margin-left:7px"〉
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