Kooperativer Bibliotheksverbund

Berlin Brandenburg

and
and

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Bleyer, Martina  (5)
Type of Medium
Language
Year
  • 1
    Language: English
    In: Journal of medical primatology, February 2014, Vol.43(1), pp.55-8
    Description: The distribution of ciliated cells in the tracheal epithelium of common marmosets was evaluated. Light and scanning electron microscopy of tracheal epithelium was performed. Ciliated cells were concentrated in cartilage-free areas and virtually absent in cartilage-supported epithelial regions. Heterogeneous distribution of ciliated cells in the trachea has to be considered when using animal models for translational respiratory research approaches.
    Keywords: Callithrix -- Anatomy & Histology ; Cilia -- Ultrastructure ; Epithelial Cells -- Cytology ; Trachea -- Cytology
    ISSN: 00472565
    E-ISSN: 1600-0684
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 2
    Language: English
    In: 2012, Vol.7(8), p.e43709
    Description: Increasing incidence and substantial morbidity and mortality of respiratory diseases requires the development of new human-specific anti-inflammatory and disease-modifying therapeutics. Therefore, new predictive animal models that closely reflect human lung pathology are needed. In the current study, a tiered acute lipopolysaccharide (LPS)-induced inflammation model was established in marmoset monkeys ( Callithrix jacchus) to reflect crucial features of inflammatory lung diseases. Firstly, in an ex vivo approach marmoset and, for the purposes of comparison, human precision-cut lung slices (PCLS) were stimulated with LPS in the presence or absence of the phosphodiesterase-4 (PDE4) inhibitor roflumilast. Pro-inflammatory cytokines including tumor necrosis factor-alpha (TNF-α) and macrophage inflammatory protein-1 beta (MIP-1β) were measured. The corticosteroid dexamethasone was used as treatment control. Secondly, in an in vivo approach marmosets were pre-treated with roflumilast or dexamethasone and unilaterally challenged with LPS. Ipsilateral bronchoalveolar lavage (BAL) was conducted 18 hours after LPS challenge. BAL fluid was processed and analyzed for neutrophils, TNF-α, and MIP-1β. TNF-α release in marmoset PCLS correlated significantly with human PCLS. Roflumilast treatment significantly reduced TNF-α secretion ex vivo in both species, with comparable half maximal inhibitory concentration (IC 50 ). LPS instillation into marmoset lungs caused a profound inflammation as shown by neutrophilic influx and increased TNF-α and MIP-1β levels in BAL fluid. This inflammatory response was significantly suppressed by roflumilast and dexamethasone. The close similarity of marmoset and human lungs regarding LPS-induced inflammation and the significant anti-inflammatory effect of approved pharmaceuticals assess the suitability of marmoset monkeys to serve as a promising model for studying anti-inflammatory drugs.
    Keywords: Research Article ; Biology ; Medicine ; Veterinary Science ; Immunology ; Pharmacology ; Respiratory Medicine
    E-ISSN: 1932-6203
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 3
    In: Journal of Medical Primatology, February 2014, Vol.43(1), pp.55-58
    Description: Background The distribution of ciliated cells in the tracheal epithelium of common marmosets was evaluated. Methods Light and scanning electron microscopy of tracheal epithelium was performed. Results Ciliated cells were concentrated in cartilage-free areas and virtually absent in cartilage-supported...
    Keywords: Ciliated Cell ; Common Marmoset ; Scanning Electron Microscopy ; Trachea
    ISSN: 0047-2565
    E-ISSN: 1600-0684
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 4
    In: Journal of Medical Primatology, April 2013, Vol.42(2), pp.79-88
    Description: Keywords: Clara cell secretory protein; electron microscopy; goblet cell; mixed type secretory cell; mucin Abstract Background The objective of this investigation was to define the phenotype and spatial distribution of Clara cells within the respiratory tract of common marmosets and to distinguish them from other non-ciliated cells (goblet cells, mixed type secretory cells). Methods Non-ciliated cells were identified immunohistochemically using antibodies against Clara cell secretory protein and mucin 5AC. Transmission electron microscopy and scanning electron microscopy were performed to characterize Clara cells ultrastructurally. Results Clara cells were present throughout the tracheobronchial tree, with lowest numbers in the trachea and highest numbers in bronchioles. Goblet cells and mixed type cells were scarce in the upper conducting airways and virtually absent within bronchioles. Ultrastructurally, Clara cells showed typical apical electron-dense granules and a prominent granular endoplasmatic reticulum. Conclusions Clara cells of common marmosets have species-specific morphological characteristics, which suggest grouping the common marmoset phenotypically between primates and rodents. Author Affiliation:
    Keywords: Clara Cell Secretory Protein ; Electron Microscopy ; Goblet Cell ; Mixed Type Secretory Cell ; Mucin
    ISSN: 0047-2565
    E-ISSN: 1600-0684
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 5
    Language: English
    In: Experimental and Toxicologic Pathology, June 2016, Vol.68(6), pp.335-343
    Description: Common marmosets ( ) are frequently used as translational animal models for human diseases. However, a comparative study of cytological and histochemical detection methods as well as morphometric and ultrastructural characterization of neutrophils and eosinophils in this species is lacking. Blood samples of house dust mite sensitized and allergen challenged as well as lipopolysaccharide (LPS) challenged marmosets were analyzed with different cytological and histological staining methods. Furthermore, cell size and number of nuclear segments were compared between neutrophils and eosinophils. Electron microscopy was performed to characterize the ultrastructure of granulocytes. Of all applied cytological stains, three allowed differentiation of eosinophils and neutrophils and, thus, reliable quantification in blood smears: May-Grünwald-Giemsa stain, Congo Red and Naphthol AS-D Chloroacetate-Esterase. For histology, Hematoxylin-Eosin (H&E) could not demonstrate clear differences, whereas Sirius Red, Congo Red, and Naphthol AS-D Chloroacetate Esterase showed capable results for identification of eosinophils or neutrophils in lung tissue. Morphometry revealed that marmoset neutrophils have more nuclear segments and are slightly larger than eosinophils. Ultrastructurally, eosinophils presented with large homogeneous electron-dense granules without crystalloid cores, while neutrophils were characterized by heterogeneous granules of different size and density. Additionally, sombrero-like vesicles were detected in tissue eosinophils of atopic marmosets, indicative for hypersensitivity-related piecemeal degranulation. In conclusion, we provide a detailed overview of marmoset eosinophils and neutrophils, important for phenotypic characterization of marmoset models for human airway diseases.
    Keywords: Common Marmoset ; Eosinophil ; Neutrophil ; Cytological Staining ; Histology ; Ultrastructural Morphology ; Medicine
    ISSN: 0940-2993
    E-ISSN: 1618-1433
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. Further information can be found on the KOBV privacy pages