Food Chemistry, 2008, Vol.107(2), pp.684-691
rhizome has long been used as an herb tonic to treat osteoporosis, arthralgia, and arthritis. The aqueous extract of . rhizome contains a high content of phenolic compounds [210.8 ± 4.6 mg catechin equivalents (CE)/g dry weight] and shows a strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity (IC = 15.93 ± 1.21 μg dry weight/ml). Further solvent partition of the aqueous extract yielded chloroform, -butanol, and water layers. Among them, -butanol layer has the highest phenol content (806.3 ± 12.3 mg CE/g dry weight) and DPPH scavenging potential (IC = 3.93 ± 0.31 μg dry weight/ml). Isolation and purification from the -butanol layer identified 12 compounds. They included four new compounds: 3′- - -hydroxybenzoylmangiferin ( ), 4′- - -hydroxybenzoylmangiferin ( ), 6′- - -hydroxybenzoylmangiferin ( ), and 3- - -hydroxybenzoylmangiferin ( ); as well as eight known compounds: mangiferin ( ), 2- -β- -xylopyranosyl-1,3,6,7-tetrahydroxyxanthone ( ), 4β-carboxymethyl-(−)-epicatechin ( ), 4β-carboxymethyl-(−)-epicatechin methyl ester ( ), eriodictyol ( ), eriodictyol-8- -β- -glucopyranoside ( ), icariside E ( ), and icariside E ( ). DPPH scavenging and Trolox equivalent antioxidant capacity (TEAC) analyses revealed that the most potent antioxidants are , , and , which exerted more than triple activity as compared with the positive controls, -tocopherol and Trolox.
Davallia Solida ; Dpph ; Trolox Equivalent Antioxidant Capacity (Teac) ; Chemistry ; Diet & Clinical Nutrition ; Economics
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